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Development of a Mucoadhesive In Situ Gelling Formulation for the Delivery of Lactobacillus gasseri into Vaginal Cavity

Local administration of vaginal probiotics, especially lactobacilli, has been recently proposed as an effective prevention strategy against candidosis recurrences, which affect 40–50% of women. In this context, the aim of the present work was the development of a mucoadhesive in situ gelling formula...

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Autores principales: Vigani, Barbara, Faccendini, Angela, Rossi, Silvia, Sandri, Giuseppina, Bonferoni, Maria Cristina, Grisoli, Pietro, Ferrari, Franca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6836057/
https://www.ncbi.nlm.nih.gov/pubmed/31623341
http://dx.doi.org/10.3390/pharmaceutics11100511
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author Vigani, Barbara
Faccendini, Angela
Rossi, Silvia
Sandri, Giuseppina
Bonferoni, Maria Cristina
Grisoli, Pietro
Ferrari, Franca
author_facet Vigani, Barbara
Faccendini, Angela
Rossi, Silvia
Sandri, Giuseppina
Bonferoni, Maria Cristina
Grisoli, Pietro
Ferrari, Franca
author_sort Vigani, Barbara
collection PubMed
description Local administration of vaginal probiotics, especially lactobacilli, has been recently proposed as an effective prevention strategy against candidosis recurrences, which affect 40–50% of women. In this context, the aim of the present work was the development of a mucoadhesive in situ gelling formulation for the vaginal administration of Lactobacillus gasseri. Mixtures of poloxamer 407 (P407) and methylcellulose (MC), two thermosensitive polymers, were prepared and subjected to rheological analyses for the assessment of their sol/gel transition temperature. The association of P407 (15% w/w) with MC (1.5% w/w) produced an increase in gelation extent at 37 °C even after dilution in simulated vaginal fluid (SVF). The presence of 0.5% w/w pectin (PEC) produced a reduction of vehicle pH and viscosity at 25 °C that is the vehicle resistance to flow during administration. The presence of a low concentration of xyloglucan (XYL) (0.25% w/w) increases the mucoadhesive properties and the capability to gelify at 37 °C of the formulation after dilution with SVF. A three-component (P407/MC/PEC; 3cM) and a four-component (P407/MC/PEC/XYL; 4cM) mixture were selected as promising candidates for the delivery of L. gasseri to the vaginal cavity. They were able to preserve L. gasseri viability and were cytocompatible towards the HeLa cell line.
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spelling pubmed-68360572019-11-25 Development of a Mucoadhesive In Situ Gelling Formulation for the Delivery of Lactobacillus gasseri into Vaginal Cavity Vigani, Barbara Faccendini, Angela Rossi, Silvia Sandri, Giuseppina Bonferoni, Maria Cristina Grisoli, Pietro Ferrari, Franca Pharmaceutics Article Local administration of vaginal probiotics, especially lactobacilli, has been recently proposed as an effective prevention strategy against candidosis recurrences, which affect 40–50% of women. In this context, the aim of the present work was the development of a mucoadhesive in situ gelling formulation for the vaginal administration of Lactobacillus gasseri. Mixtures of poloxamer 407 (P407) and methylcellulose (MC), two thermosensitive polymers, were prepared and subjected to rheological analyses for the assessment of their sol/gel transition temperature. The association of P407 (15% w/w) with MC (1.5% w/w) produced an increase in gelation extent at 37 °C even after dilution in simulated vaginal fluid (SVF). The presence of 0.5% w/w pectin (PEC) produced a reduction of vehicle pH and viscosity at 25 °C that is the vehicle resistance to flow during administration. The presence of a low concentration of xyloglucan (XYL) (0.25% w/w) increases the mucoadhesive properties and the capability to gelify at 37 °C of the formulation after dilution with SVF. A three-component (P407/MC/PEC; 3cM) and a four-component (P407/MC/PEC/XYL; 4cM) mixture were selected as promising candidates for the delivery of L. gasseri to the vaginal cavity. They were able to preserve L. gasseri viability and were cytocompatible towards the HeLa cell line. MDPI 2019-10-03 /pmc/articles/PMC6836057/ /pubmed/31623341 http://dx.doi.org/10.3390/pharmaceutics11100511 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vigani, Barbara
Faccendini, Angela
Rossi, Silvia
Sandri, Giuseppina
Bonferoni, Maria Cristina
Grisoli, Pietro
Ferrari, Franca
Development of a Mucoadhesive In Situ Gelling Formulation for the Delivery of Lactobacillus gasseri into Vaginal Cavity
title Development of a Mucoadhesive In Situ Gelling Formulation for the Delivery of Lactobacillus gasseri into Vaginal Cavity
title_full Development of a Mucoadhesive In Situ Gelling Formulation for the Delivery of Lactobacillus gasseri into Vaginal Cavity
title_fullStr Development of a Mucoadhesive In Situ Gelling Formulation for the Delivery of Lactobacillus gasseri into Vaginal Cavity
title_full_unstemmed Development of a Mucoadhesive In Situ Gelling Formulation for the Delivery of Lactobacillus gasseri into Vaginal Cavity
title_short Development of a Mucoadhesive In Situ Gelling Formulation for the Delivery of Lactobacillus gasseri into Vaginal Cavity
title_sort development of a mucoadhesive in situ gelling formulation for the delivery of lactobacillus gasseri into vaginal cavity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6836057/
https://www.ncbi.nlm.nih.gov/pubmed/31623341
http://dx.doi.org/10.3390/pharmaceutics11100511
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