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Single-Dimer Formation Rate Reveals Heterogeneous Particle Surface Reactivity

[Image: see text] Biofunctionalized micro- and nanoparticles are important for a wide range of applications, but methodologies to measure, modulate, and model interactions between individual particles are scarce. Here, we describe a technique to measure the aggregation rate of two particles to a sin...

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Autores principales: Scheepers, M. R. W., van IJzendoorn, L. J., Prins, M. W. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2019
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6836307/
https://www.ncbi.nlm.nih.gov/pubmed/31607127
http://dx.doi.org/10.1021/acs.langmuir.9b02199
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author Scheepers, M. R. W.
van IJzendoorn, L. J.
Prins, M. W. J.
author_facet Scheepers, M. R. W.
van IJzendoorn, L. J.
Prins, M. W. J.
author_sort Scheepers, M. R. W.
collection PubMed
description [Image: see text] Biofunctionalized micro- and nanoparticles are important for a wide range of applications, but methodologies to measure, modulate, and model interactions between individual particles are scarce. Here, we describe a technique to measure the aggregation rate of two particles to a single dimer, by recording the trajectory that a particle follows on the surface of another particle as a function of time. The trajectory and the interparticle potential are controlled by a magnetic field. Particles were studied with and without conjugated antibodies in a wide range of pH conditions. The data shows that the aggregation process strongly depends on the particle surface charge density and hardly on the antibody surface coverage. Furthermore, microscopy videos of single particle dimers reveal the presence of reactive patches and thus heterogeneity in the particle surface reactivity. The aggregation rates measured with the single-dimer experiment are compared to data from an ensemble aggregation experiment. Quantitative agreement is obtained using a model that includes the influence of surface heterogeneity on particle aggregation. This single-dimer experiment clarifies how heterogeneities in particle reactivity play a role in colloidal stability.
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spelling pubmed-68363072019-11-08 Single-Dimer Formation Rate Reveals Heterogeneous Particle Surface Reactivity Scheepers, M. R. W. van IJzendoorn, L. J. Prins, M. W. J. Langmuir [Image: see text] Biofunctionalized micro- and nanoparticles are important for a wide range of applications, but methodologies to measure, modulate, and model interactions between individual particles are scarce. Here, we describe a technique to measure the aggregation rate of two particles to a single dimer, by recording the trajectory that a particle follows on the surface of another particle as a function of time. The trajectory and the interparticle potential are controlled by a magnetic field. Particles were studied with and without conjugated antibodies in a wide range of pH conditions. The data shows that the aggregation process strongly depends on the particle surface charge density and hardly on the antibody surface coverage. Furthermore, microscopy videos of single particle dimers reveal the presence of reactive patches and thus heterogeneity in the particle surface reactivity. The aggregation rates measured with the single-dimer experiment are compared to data from an ensemble aggregation experiment. Quantitative agreement is obtained using a model that includes the influence of surface heterogeneity on particle aggregation. This single-dimer experiment clarifies how heterogeneities in particle reactivity play a role in colloidal stability. American Chemical Society 2019-10-13 2019-11-05 /pmc/articles/PMC6836307/ /pubmed/31607127 http://dx.doi.org/10.1021/acs.langmuir.9b02199 Text en Copyright © 2019 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.
spellingShingle Scheepers, M. R. W.
van IJzendoorn, L. J.
Prins, M. W. J.
Single-Dimer Formation Rate Reveals Heterogeneous Particle Surface Reactivity
title Single-Dimer Formation Rate Reveals Heterogeneous Particle Surface Reactivity
title_full Single-Dimer Formation Rate Reveals Heterogeneous Particle Surface Reactivity
title_fullStr Single-Dimer Formation Rate Reveals Heterogeneous Particle Surface Reactivity
title_full_unstemmed Single-Dimer Formation Rate Reveals Heterogeneous Particle Surface Reactivity
title_short Single-Dimer Formation Rate Reveals Heterogeneous Particle Surface Reactivity
title_sort single-dimer formation rate reveals heterogeneous particle surface reactivity
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6836307/
https://www.ncbi.nlm.nih.gov/pubmed/31607127
http://dx.doi.org/10.1021/acs.langmuir.9b02199
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