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Low pH effects on reactive oxygen species and methylglyoxal metabolisms in Citrus roots and leaves

BACKGROUND: Limited data are available on the responses of reactive oxygen species (ROS) and methylglyoxal (MG) metabolisms to low pH in roots and leaves. In China, quite a few of Citrus are cultivated in acidic soils (pH < 5.0). ‘Xuegan’ (Citrus sinensis) and ‘Sour pummelo’ (Citrus grandis) (C....

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Autores principales: Long, An, Huang, Wei-Lin, Qi, Yi-Ping, Yang, Lin-Tong, Lai, Ning-Wei, Guo, Jiu-Xin, Chen, Li-Song
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6836343/
https://www.ncbi.nlm.nih.gov/pubmed/31694545
http://dx.doi.org/10.1186/s12870-019-2103-5
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author Long, An
Huang, Wei-Lin
Qi, Yi-Ping
Yang, Lin-Tong
Lai, Ning-Wei
Guo, Jiu-Xin
Chen, Li-Song
author_facet Long, An
Huang, Wei-Lin
Qi, Yi-Ping
Yang, Lin-Tong
Lai, Ning-Wei
Guo, Jiu-Xin
Chen, Li-Song
author_sort Long, An
collection PubMed
description BACKGROUND: Limited data are available on the responses of reactive oxygen species (ROS) and methylglyoxal (MG) metabolisms to low pH in roots and leaves. In China, quite a few of Citrus are cultivated in acidic soils (pH < 5.0). ‘Xuegan’ (Citrus sinensis) and ‘Sour pummelo’ (Citrus grandis) (C. sinensis were more tolerant to low pH than C. grandis) seedlings were irrigated daily with nutrient solution at a pH of 2.5, 3 or 5 for nine months. Thereafter, we examined low pH effects on growth, and superoxide anion production rate (SAP), malondialdehyde (MDA), MG, antioxidants, and enzymes related to ROS and MG detoxification in roots and leaves in order to (a) test the hypothesis that low pH affected ROS and MG metabolisms more in roots than those of leaves, and (b) understand the roles of ROS and MG metabolisms in Citrus low pH-tolerance and -toxicity. RESULTS: Compared with control, most of the physiological parameters related to ROS and MG metabolisms were greatly altered at pH 2.5, but almost unaffected at pH 3. In addition to decreased root growth, many fibrous roots became rotten and died at pH 2.5. pH 2.5-induced changes in SAP, the levels of MDA, MG and antioxidants, and the activities of most enzymes related to ROS and MG metabolisms were greater in roots than those of leaves. Impairment of root ascorbate metabolism was the most serious, especially in C. grandis roots. pH 2.5-induced increases in MDA and MG levels in roots and leaves, decreases in the ratios of ascorbate/(ascorbate+dehydroascorbate) in roots and leaves and of reduced glutathione/(reduced+oxidized glutathione) in roots were greater in C. grandis than those in C. sinensis. CONCLUSIONS: Low pH affected MG and ROS metabolisms more in roots than those in leaves. The most seriously impaired ascorbate metabolism in roots was suggested to play a role in low pH-induced root death and growth inhibition. Low pH-treated C. sinensis roots and leaves had higher capacity to maintain a balance between ROS and MG production and their removal via detoxification systems than low pH-treated C. grandis ones, thus contribute to the higher acid-tolerance of C. sinensis.
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spelling pubmed-68363432019-11-08 Low pH effects on reactive oxygen species and methylglyoxal metabolisms in Citrus roots and leaves Long, An Huang, Wei-Lin Qi, Yi-Ping Yang, Lin-Tong Lai, Ning-Wei Guo, Jiu-Xin Chen, Li-Song BMC Plant Biol Research Article BACKGROUND: Limited data are available on the responses of reactive oxygen species (ROS) and methylglyoxal (MG) metabolisms to low pH in roots and leaves. In China, quite a few of Citrus are cultivated in acidic soils (pH < 5.0). ‘Xuegan’ (Citrus sinensis) and ‘Sour pummelo’ (Citrus grandis) (C. sinensis were more tolerant to low pH than C. grandis) seedlings were irrigated daily with nutrient solution at a pH of 2.5, 3 or 5 for nine months. Thereafter, we examined low pH effects on growth, and superoxide anion production rate (SAP), malondialdehyde (MDA), MG, antioxidants, and enzymes related to ROS and MG detoxification in roots and leaves in order to (a) test the hypothesis that low pH affected ROS and MG metabolisms more in roots than those of leaves, and (b) understand the roles of ROS and MG metabolisms in Citrus low pH-tolerance and -toxicity. RESULTS: Compared with control, most of the physiological parameters related to ROS and MG metabolisms were greatly altered at pH 2.5, but almost unaffected at pH 3. In addition to decreased root growth, many fibrous roots became rotten and died at pH 2.5. pH 2.5-induced changes in SAP, the levels of MDA, MG and antioxidants, and the activities of most enzymes related to ROS and MG metabolisms were greater in roots than those of leaves. Impairment of root ascorbate metabolism was the most serious, especially in C. grandis roots. pH 2.5-induced increases in MDA and MG levels in roots and leaves, decreases in the ratios of ascorbate/(ascorbate+dehydroascorbate) in roots and leaves and of reduced glutathione/(reduced+oxidized glutathione) in roots were greater in C. grandis than those in C. sinensis. CONCLUSIONS: Low pH affected MG and ROS metabolisms more in roots than those in leaves. The most seriously impaired ascorbate metabolism in roots was suggested to play a role in low pH-induced root death and growth inhibition. Low pH-treated C. sinensis roots and leaves had higher capacity to maintain a balance between ROS and MG production and their removal via detoxification systems than low pH-treated C. grandis ones, thus contribute to the higher acid-tolerance of C. sinensis. BioMed Central 2019-11-06 /pmc/articles/PMC6836343/ /pubmed/31694545 http://dx.doi.org/10.1186/s12870-019-2103-5 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Long, An
Huang, Wei-Lin
Qi, Yi-Ping
Yang, Lin-Tong
Lai, Ning-Wei
Guo, Jiu-Xin
Chen, Li-Song
Low pH effects on reactive oxygen species and methylglyoxal metabolisms in Citrus roots and leaves
title Low pH effects on reactive oxygen species and methylglyoxal metabolisms in Citrus roots and leaves
title_full Low pH effects on reactive oxygen species and methylglyoxal metabolisms in Citrus roots and leaves
title_fullStr Low pH effects on reactive oxygen species and methylglyoxal metabolisms in Citrus roots and leaves
title_full_unstemmed Low pH effects on reactive oxygen species and methylglyoxal metabolisms in Citrus roots and leaves
title_short Low pH effects on reactive oxygen species and methylglyoxal metabolisms in Citrus roots and leaves
title_sort low ph effects on reactive oxygen species and methylglyoxal metabolisms in citrus roots and leaves
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6836343/
https://www.ncbi.nlm.nih.gov/pubmed/31694545
http://dx.doi.org/10.1186/s12870-019-2103-5
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