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Novel nose poke-based temporal discrimination tasks with concurrent in vivo calcium imaging in freely moving mice

The hippocampus has been known to process temporal information as part of memory formation. While time cells have been observed in the hippocampus and medial entorhinal cortex, a number of the behavioral tasks used present potential confounds that may cause some contamination of time cell observatio...

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Autores principales: Marks, William D., Osanai, Hisayuki, Yamamoto, Jun, Ogawa, Sachie K., Kitamura, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6836444/
https://www.ncbi.nlm.nih.gov/pubmed/31694671
http://dx.doi.org/10.1186/s13041-019-0515-7
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author Marks, William D.
Osanai, Hisayuki
Yamamoto, Jun
Ogawa, Sachie K.
Kitamura, Takashi
author_facet Marks, William D.
Osanai, Hisayuki
Yamamoto, Jun
Ogawa, Sachie K.
Kitamura, Takashi
author_sort Marks, William D.
collection PubMed
description The hippocampus has been known to process temporal information as part of memory formation. While time cells have been observed in the hippocampus and medial entorhinal cortex, a number of the behavioral tasks used present potential confounds that may cause some contamination of time cell observations due to animal movement. Here, we report the development of a novel nose poke-based temporal discrimination task designed to be used with in vivo calcium imaging for the analysis of hippocampal time cells in freely moving mice. First, we developed a ten second held nose poke paradigm for use in mice to deliver a purer time metric for the analysis of time cell activity in hippocampus CA1. Second, we developed a temporal discrimination task that involves the association of held nose poke durations of differing lengths with differential spatial cues presented in arms on a linear I-maze. Four of five mice achieved successful temporal discrimination within three weeks. Calcium imaging has been successfully performed in each of these tasks, with time cell activity being detected in the 10s nose poke task, and calcium waves being observed in discrete components of the temporal discrimination task. The newly developed behavior tasks in mice serve as novel tools to accelerate the study of time cell activity and examine the integration of time and space in episodic memory formation.
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spelling pubmed-68364442019-11-08 Novel nose poke-based temporal discrimination tasks with concurrent in vivo calcium imaging in freely moving mice Marks, William D. Osanai, Hisayuki Yamamoto, Jun Ogawa, Sachie K. Kitamura, Takashi Mol Brain Micro Report The hippocampus has been known to process temporal information as part of memory formation. While time cells have been observed in the hippocampus and medial entorhinal cortex, a number of the behavioral tasks used present potential confounds that may cause some contamination of time cell observations due to animal movement. Here, we report the development of a novel nose poke-based temporal discrimination task designed to be used with in vivo calcium imaging for the analysis of hippocampal time cells in freely moving mice. First, we developed a ten second held nose poke paradigm for use in mice to deliver a purer time metric for the analysis of time cell activity in hippocampus CA1. Second, we developed a temporal discrimination task that involves the association of held nose poke durations of differing lengths with differential spatial cues presented in arms on a linear I-maze. Four of five mice achieved successful temporal discrimination within three weeks. Calcium imaging has been successfully performed in each of these tasks, with time cell activity being detected in the 10s nose poke task, and calcium waves being observed in discrete components of the temporal discrimination task. The newly developed behavior tasks in mice serve as novel tools to accelerate the study of time cell activity and examine the integration of time and space in episodic memory formation. BioMed Central 2019-11-06 /pmc/articles/PMC6836444/ /pubmed/31694671 http://dx.doi.org/10.1186/s13041-019-0515-7 Text en © The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Micro Report
Marks, William D.
Osanai, Hisayuki
Yamamoto, Jun
Ogawa, Sachie K.
Kitamura, Takashi
Novel nose poke-based temporal discrimination tasks with concurrent in vivo calcium imaging in freely moving mice
title Novel nose poke-based temporal discrimination tasks with concurrent in vivo calcium imaging in freely moving mice
title_full Novel nose poke-based temporal discrimination tasks with concurrent in vivo calcium imaging in freely moving mice
title_fullStr Novel nose poke-based temporal discrimination tasks with concurrent in vivo calcium imaging in freely moving mice
title_full_unstemmed Novel nose poke-based temporal discrimination tasks with concurrent in vivo calcium imaging in freely moving mice
title_short Novel nose poke-based temporal discrimination tasks with concurrent in vivo calcium imaging in freely moving mice
title_sort novel nose poke-based temporal discrimination tasks with concurrent in vivo calcium imaging in freely moving mice
topic Micro Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6836444/
https://www.ncbi.nlm.nih.gov/pubmed/31694671
http://dx.doi.org/10.1186/s13041-019-0515-7
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