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Limited Regeneration Potential with Minimal Epicardial Progenitor Conversions in the Neonatal Mouse Heart after Injury
The regeneration capacity of neonatal mouse heart is controversial. In addition, whether epicardial cells provide a progenitor pool for de novo heart regeneration is incompletely defined. Following apical resection of the neonatal mouse heart, we observed limited regeneration potential. Fate-mapping...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6837841/ https://www.ncbi.nlm.nih.gov/pubmed/31269439 http://dx.doi.org/10.1016/j.celrep.2019.06.003 |
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author | Cai, Weibin Tan, Jing Yan, Jianyun Zhang, Lu Cai, Xiaoqiang Wang, Haiping Liu, Fang Ye, Maoqing Cai, Chen-Leng |
author_facet | Cai, Weibin Tan, Jing Yan, Jianyun Zhang, Lu Cai, Xiaoqiang Wang, Haiping Liu, Fang Ye, Maoqing Cai, Chen-Leng |
author_sort | Cai, Weibin |
collection | PubMed |
description | The regeneration capacity of neonatal mouse heart is controversial. In addition, whether epicardial cells provide a progenitor pool for de novo heart regeneration is incompletely defined. Following apical resection of the neonatal mouse heart, we observed limited regeneration potential. Fate-mapping of Tbx18(MerCreMer) mice revealed that newly formed coronary vessels and a limited number of cardiomyocytes were derived from the T-box transcription factor 18 (Tbx18) lineage. However, further lineage tracing with SM-MHC(CreERT2) and Nfactc1(Cre) mice revealed that the new smooth muscle and endothelial cells are in fact derivatives of pre-existing coronary vessels. Our data show that neonatal mouse heart can regenerate but that its potential is limited. Moreover, although epicardial cells are multipotent during embryogenesis, their contribution to heart repair through “stem” or “progenitor” cell conversion is minimal after birth. These observations suggest that early embryonic heart development and postnatal heart regeneration are distinct biological processes. Multipotency of epicardial cells is significantly decreased after birth. |
format | Online Article Text |
id | pubmed-6837841 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
record_format | MEDLINE/PubMed |
spelling | pubmed-68378412019-11-07 Limited Regeneration Potential with Minimal Epicardial Progenitor Conversions in the Neonatal Mouse Heart after Injury Cai, Weibin Tan, Jing Yan, Jianyun Zhang, Lu Cai, Xiaoqiang Wang, Haiping Liu, Fang Ye, Maoqing Cai, Chen-Leng Cell Rep Article The regeneration capacity of neonatal mouse heart is controversial. In addition, whether epicardial cells provide a progenitor pool for de novo heart regeneration is incompletely defined. Following apical resection of the neonatal mouse heart, we observed limited regeneration potential. Fate-mapping of Tbx18(MerCreMer) mice revealed that newly formed coronary vessels and a limited number of cardiomyocytes were derived from the T-box transcription factor 18 (Tbx18) lineage. However, further lineage tracing with SM-MHC(CreERT2) and Nfactc1(Cre) mice revealed that the new smooth muscle and endothelial cells are in fact derivatives of pre-existing coronary vessels. Our data show that neonatal mouse heart can regenerate but that its potential is limited. Moreover, although epicardial cells are multipotent during embryogenesis, their contribution to heart repair through “stem” or “progenitor” cell conversion is minimal after birth. These observations suggest that early embryonic heart development and postnatal heart regeneration are distinct biological processes. Multipotency of epicardial cells is significantly decreased after birth. 2019-07-02 /pmc/articles/PMC6837841/ /pubmed/31269439 http://dx.doi.org/10.1016/j.celrep.2019.06.003 Text en This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cai, Weibin Tan, Jing Yan, Jianyun Zhang, Lu Cai, Xiaoqiang Wang, Haiping Liu, Fang Ye, Maoqing Cai, Chen-Leng Limited Regeneration Potential with Minimal Epicardial Progenitor Conversions in the Neonatal Mouse Heart after Injury |
title | Limited Regeneration Potential with Minimal Epicardial Progenitor Conversions in the Neonatal Mouse Heart after Injury |
title_full | Limited Regeneration Potential with Minimal Epicardial Progenitor Conversions in the Neonatal Mouse Heart after Injury |
title_fullStr | Limited Regeneration Potential with Minimal Epicardial Progenitor Conversions in the Neonatal Mouse Heart after Injury |
title_full_unstemmed | Limited Regeneration Potential with Minimal Epicardial Progenitor Conversions in the Neonatal Mouse Heart after Injury |
title_short | Limited Regeneration Potential with Minimal Epicardial Progenitor Conversions in the Neonatal Mouse Heart after Injury |
title_sort | limited regeneration potential with minimal epicardial progenitor conversions in the neonatal mouse heart after injury |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6837841/ https://www.ncbi.nlm.nih.gov/pubmed/31269439 http://dx.doi.org/10.1016/j.celrep.2019.06.003 |
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