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Transverse tubule remodeling enhances Orai1-dependent Ca(2+) entry in skeletal muscle

Exercise promotes the formation of intracellular junctions in skeletal muscle between stacks of sarcoplasmic reticulum (SR) cisternae and extensions of transverse-tubules (TT) that increase co-localization of proteins required for store-operated Ca(2+) entry (SOCE). Here, we report that SOCE, peak C...

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Detalles Bibliográficos
Autores principales: Michelucci, Antonio, Boncompagni, Simona, Pietrangelo, Laura, García-Castañeda, Maricela, Takano, Takahiro, Malik, Sundeep, Dirksen, Robert T, Protasi, Feliciano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6837846/
https://www.ncbi.nlm.nih.gov/pubmed/31657717
http://dx.doi.org/10.7554/eLife.47576
Descripción
Sumario:Exercise promotes the formation of intracellular junctions in skeletal muscle between stacks of sarcoplasmic reticulum (SR) cisternae and extensions of transverse-tubules (TT) that increase co-localization of proteins required for store-operated Ca(2+) entry (SOCE). Here, we report that SOCE, peak Ca(2+) transient amplitude and muscle force production during repetitive stimulation are increased after exercise in parallel with the time course of TT association with SR-stacks. Unexpectedly, exercise also activated constitutive Ca(2+) entry coincident with a modest decrease in total releasable Ca(2+) store content. Importantly, this decrease in releasable Ca(2+) store content observed after exercise was reversed by repetitive high-frequency stimulation, consistent with enhanced SOCE. The functional benefits of exercise on SOCE, constitutive Ca(2+) entry and muscle force production were lost in mice with muscle-specific loss of Orai1 function. These results indicate that TT association with SR-stacks enhances Orai1-dependent SOCE to optimize Ca(2+) dynamics and muscle contractile function during acute exercise.