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A New Chloroplast DNA Extraction Protocol Significantly Improves the Chloroplast Genome Sequence Quality of Foxtail Millet (Setaria italica (L.) P. Beauv.)

The complexity of the leaf constitution of foxtail millet (Setaria italica (L.) P. Beauv.) makes it difficult to obtain high-purity cpDNA. Here, we developed a protocol to isolate high-quality cpDNA from foxtail millet and other crops. The new protocol replaces previous tissue grinding and homogeniz...

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Detalles Bibliográficos
Autores principales: Liu, Dan, Cui, Yanjiao, Li, Suying, Bai, Guihua, Li, Qiang, Zhao, Zilong, Liang, Dan, Wang, Conglei, Wang, Jianhe, Shi, Xiaowei, Chen, Chao, Feng, Gang, Liu, Zhengli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6838068/
https://www.ncbi.nlm.nih.gov/pubmed/31700055
http://dx.doi.org/10.1038/s41598-019-52786-2