Selecting reference genes in RT-qPCR based on equivalence tests: a network based approach

Because quantitative reverse transcription PCR (RT-qPCR) gene expression data are compositional, amounts of quantified RNAs must be normalized using reference genes. However, the two most used methods to select reference genes (NormFinder and geNorm) ignore the compositional nature of RT-qPCR data,...

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Detalles Bibliográficos
Autores principales: Curis, Emmanuel, Nepost, Calypso, Grillault Laroche, Diane, Courtin, Cindie, Laplanche, Jean-Louis, Etain, Bruno, Marie-Claire, Cynthia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6838083/
https://www.ncbi.nlm.nih.gov/pubmed/31700128
http://dx.doi.org/10.1038/s41598-019-52217-2

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