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Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridisation

Parasexuality contributes to diversity and adaptive evolution of haploid (monokaryotic) fungi. However, non-sexual genetic exchange mechanisms are not defined in dikaryotic fungi (containing two distinct haploid nuclei). Newly emerged strains of the wheat stem rust pathogen, Puccinia graminis f. sp....

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Autores principales: Li, Feng, Upadhyaya, Narayana M., Sperschneider, Jana, Matny, Oadi, Nguyen-Phuc, Hoa, Mago, Rohit, Raley, Castle, Miller, Marisa E., Silverstein, Kevin A. T., Henningsen, Eva, Hirsch, Cory D., Visser, Botma, Pretorius, Zacharias A., Steffenson, Brian J., Schwessinger, Benjamin, Dodds, Peter N., Figueroa, Melania
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6838127/
https://www.ncbi.nlm.nih.gov/pubmed/31699975
http://dx.doi.org/10.1038/s41467-019-12927-7
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author Li, Feng
Upadhyaya, Narayana M.
Sperschneider, Jana
Matny, Oadi
Nguyen-Phuc, Hoa
Mago, Rohit
Raley, Castle
Miller, Marisa E.
Silverstein, Kevin A. T.
Henningsen, Eva
Hirsch, Cory D.
Visser, Botma
Pretorius, Zacharias A.
Steffenson, Brian J.
Schwessinger, Benjamin
Dodds, Peter N.
Figueroa, Melania
author_facet Li, Feng
Upadhyaya, Narayana M.
Sperschneider, Jana
Matny, Oadi
Nguyen-Phuc, Hoa
Mago, Rohit
Raley, Castle
Miller, Marisa E.
Silverstein, Kevin A. T.
Henningsen, Eva
Hirsch, Cory D.
Visser, Botma
Pretorius, Zacharias A.
Steffenson, Brian J.
Schwessinger, Benjamin
Dodds, Peter N.
Figueroa, Melania
author_sort Li, Feng
collection PubMed
description Parasexuality contributes to diversity and adaptive evolution of haploid (monokaryotic) fungi. However, non-sexual genetic exchange mechanisms are not defined in dikaryotic fungi (containing two distinct haploid nuclei). Newly emerged strains of the wheat stem rust pathogen, Puccinia graminis f. sp. tritici (Pgt), such as Ug99, are a major threat to global food security. Here, we provide genomics-based evidence supporting that Ug99 arose by somatic hybridisation and nuclear exchange between dikaryons. Fully haplotype-resolved genome assembly and DNA proximity analysis reveal that Ug99 shares one haploid nucleus genotype with a much older African lineage of Pgt, with no recombination or chromosome reassortment. These findings indicate that nuclear exchange between dikaryotes can generate genetic diversity and facilitate the emergence of new lineages in asexual fungal populations.
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spelling pubmed-68381272019-11-12 Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridisation Li, Feng Upadhyaya, Narayana M. Sperschneider, Jana Matny, Oadi Nguyen-Phuc, Hoa Mago, Rohit Raley, Castle Miller, Marisa E. Silverstein, Kevin A. T. Henningsen, Eva Hirsch, Cory D. Visser, Botma Pretorius, Zacharias A. Steffenson, Brian J. Schwessinger, Benjamin Dodds, Peter N. Figueroa, Melania Nat Commun Article Parasexuality contributes to diversity and adaptive evolution of haploid (monokaryotic) fungi. However, non-sexual genetic exchange mechanisms are not defined in dikaryotic fungi (containing two distinct haploid nuclei). Newly emerged strains of the wheat stem rust pathogen, Puccinia graminis f. sp. tritici (Pgt), such as Ug99, are a major threat to global food security. Here, we provide genomics-based evidence supporting that Ug99 arose by somatic hybridisation and nuclear exchange between dikaryons. Fully haplotype-resolved genome assembly and DNA proximity analysis reveal that Ug99 shares one haploid nucleus genotype with a much older African lineage of Pgt, with no recombination or chromosome reassortment. These findings indicate that nuclear exchange between dikaryotes can generate genetic diversity and facilitate the emergence of new lineages in asexual fungal populations. Nature Publishing Group UK 2019-11-07 /pmc/articles/PMC6838127/ /pubmed/31699975 http://dx.doi.org/10.1038/s41467-019-12927-7 Text en © Crown 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Li, Feng
Upadhyaya, Narayana M.
Sperschneider, Jana
Matny, Oadi
Nguyen-Phuc, Hoa
Mago, Rohit
Raley, Castle
Miller, Marisa E.
Silverstein, Kevin A. T.
Henningsen, Eva
Hirsch, Cory D.
Visser, Botma
Pretorius, Zacharias A.
Steffenson, Brian J.
Schwessinger, Benjamin
Dodds, Peter N.
Figueroa, Melania
Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridisation
title Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridisation
title_full Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridisation
title_fullStr Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridisation
title_full_unstemmed Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridisation
title_short Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridisation
title_sort emergence of the ug99 lineage of the wheat stem rust pathogen through somatic hybridisation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6838127/
https://www.ncbi.nlm.nih.gov/pubmed/31699975
http://dx.doi.org/10.1038/s41467-019-12927-7
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