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Identification of Serum Exosomal hsa-circ-0004771 as a Novel Diagnostic Biomarker of Colorectal Cancer

Background: Exosomal circular RNAs (circRNAs) in peripheral blood are considered as emerging diagnostic biomarkers of cancers. Owing to the lack of sensitive and specific biomarkers, a large number of colorectal cancer (CRC) patients were diagnosed in advanced stages leading to high mortality. This...

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Autores principales: Pan, Bei, Qin, Jian, Liu, Xiangxiang, He, Bangshun, Wang, Xuhong, Pan, Yuqin, Sun, Huiling, Xu, Tao, Xu, Mu, Chen, Xiaoxiang, Xu, Xueni, Zeng, Kaixuan, Sun, Li, Wang, Shukui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6838203/
https://www.ncbi.nlm.nih.gov/pubmed/31737058
http://dx.doi.org/10.3389/fgene.2019.01096
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author Pan, Bei
Qin, Jian
Liu, Xiangxiang
He, Bangshun
Wang, Xuhong
Pan, Yuqin
Sun, Huiling
Xu, Tao
Xu, Mu
Chen, Xiaoxiang
Xu, Xueni
Zeng, Kaixuan
Sun, Li
Wang, Shukui
author_facet Pan, Bei
Qin, Jian
Liu, Xiangxiang
He, Bangshun
Wang, Xuhong
Pan, Yuqin
Sun, Huiling
Xu, Tao
Xu, Mu
Chen, Xiaoxiang
Xu, Xueni
Zeng, Kaixuan
Sun, Li
Wang, Shukui
author_sort Pan, Bei
collection PubMed
description Background: Exosomal circular RNAs (circRNAs) in peripheral blood are considered as emerging diagnostic biomarkers of cancers. Owing to the lack of sensitive and specific biomarkers, a large number of colorectal cancer (CRC) patients were diagnosed in advanced stages leading to high mortality. This study aimed to identify circulating exosomal circRNAs as novel diagnostic biomarkers of CRC. Materials and Methods: Candidate circRNA was selected by integrating analysis of Gene Expression Omnibus (GEO) database with online program GEO2R. A total of 170 patients and 45 healthy controls were enrolled to assess the diagnostic value of circRNAs for CRC. Exosomes isolated from the serum of participants and cell cultured media were confirmed by transmission electron microscope (TEM), Nanoparticle Tracking Analysis and western blot. The expression and the diagnostic utility of circRNA were tested by qRT-PCR and receiver operating characteristic (ROC) analysis, respectively. Results: The circulating exosomal hsa-circ-0004771 with most abundant among the top ten differentially expressed circRNAs (fold change ≥1.5) was selected for further study based on the results of GEO dataset analysis. The up-regulated exosomal hsa-circ-0004771 was verified in serum of CRC patients compared to healthy controls (HCs) and patients with benign intestinal diseases (BIDs) by qRT-PCR. The area under the ROC curves (AUCs) of circulating exosomal hsa-circ-0004771 were 0.59 (95%CI, 0.457–0.725), 0.86 (95%CI, 0.785–0.933) and 0.88 (95%CI, 0.815–0.940) to differentiate BIDs, stage I/II CRC patients and CRC patients from HCs, respectively. The AUC was 0.816 (95%CI, 0.728–0.9) to differentiate stage I/II CRC patients from patients with BIDs. In addition, the elevated expression of exosomal hsa-circ-0004771 in the serum of CRC patients was tumor-derived. It was found that the expression of exosomal hsa-circ-0004771 was down-regulated expression of in the serum of postoperative CRC patients as well as cultured media of CRC cells treated with GW4869. Conclusions: Circulating exosomal hsa-circ-0004771 was significantly up-regulated in CRC patients and served as a novel potential diagnostic biomarker of CRC.
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spelling pubmed-68382032019-11-15 Identification of Serum Exosomal hsa-circ-0004771 as a Novel Diagnostic Biomarker of Colorectal Cancer Pan, Bei Qin, Jian Liu, Xiangxiang He, Bangshun Wang, Xuhong Pan, Yuqin Sun, Huiling Xu, Tao Xu, Mu Chen, Xiaoxiang Xu, Xueni Zeng, Kaixuan Sun, Li Wang, Shukui Front Genet Genetics Background: Exosomal circular RNAs (circRNAs) in peripheral blood are considered as emerging diagnostic biomarkers of cancers. Owing to the lack of sensitive and specific biomarkers, a large number of colorectal cancer (CRC) patients were diagnosed in advanced stages leading to high mortality. This study aimed to identify circulating exosomal circRNAs as novel diagnostic biomarkers of CRC. Materials and Methods: Candidate circRNA was selected by integrating analysis of Gene Expression Omnibus (GEO) database with online program GEO2R. A total of 170 patients and 45 healthy controls were enrolled to assess the diagnostic value of circRNAs for CRC. Exosomes isolated from the serum of participants and cell cultured media were confirmed by transmission electron microscope (TEM), Nanoparticle Tracking Analysis and western blot. The expression and the diagnostic utility of circRNA were tested by qRT-PCR and receiver operating characteristic (ROC) analysis, respectively. Results: The circulating exosomal hsa-circ-0004771 with most abundant among the top ten differentially expressed circRNAs (fold change ≥1.5) was selected for further study based on the results of GEO dataset analysis. The up-regulated exosomal hsa-circ-0004771 was verified in serum of CRC patients compared to healthy controls (HCs) and patients with benign intestinal diseases (BIDs) by qRT-PCR. The area under the ROC curves (AUCs) of circulating exosomal hsa-circ-0004771 were 0.59 (95%CI, 0.457–0.725), 0.86 (95%CI, 0.785–0.933) and 0.88 (95%CI, 0.815–0.940) to differentiate BIDs, stage I/II CRC patients and CRC patients from HCs, respectively. The AUC was 0.816 (95%CI, 0.728–0.9) to differentiate stage I/II CRC patients from patients with BIDs. In addition, the elevated expression of exosomal hsa-circ-0004771 in the serum of CRC patients was tumor-derived. It was found that the expression of exosomal hsa-circ-0004771 was down-regulated expression of in the serum of postoperative CRC patients as well as cultured media of CRC cells treated with GW4869. Conclusions: Circulating exosomal hsa-circ-0004771 was significantly up-regulated in CRC patients and served as a novel potential diagnostic biomarker of CRC. Frontiers Media S.A. 2019-11-01 /pmc/articles/PMC6838203/ /pubmed/31737058 http://dx.doi.org/10.3389/fgene.2019.01096 Text en Copyright © 2019 Pan, Qin, Liu, He, Wang, Pan, Sun, Xu, Xu, Chen, Xu, Zeng, Sun and Wang http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Pan, Bei
Qin, Jian
Liu, Xiangxiang
He, Bangshun
Wang, Xuhong
Pan, Yuqin
Sun, Huiling
Xu, Tao
Xu, Mu
Chen, Xiaoxiang
Xu, Xueni
Zeng, Kaixuan
Sun, Li
Wang, Shukui
Identification of Serum Exosomal hsa-circ-0004771 as a Novel Diagnostic Biomarker of Colorectal Cancer
title Identification of Serum Exosomal hsa-circ-0004771 as a Novel Diagnostic Biomarker of Colorectal Cancer
title_full Identification of Serum Exosomal hsa-circ-0004771 as a Novel Diagnostic Biomarker of Colorectal Cancer
title_fullStr Identification of Serum Exosomal hsa-circ-0004771 as a Novel Diagnostic Biomarker of Colorectal Cancer
title_full_unstemmed Identification of Serum Exosomal hsa-circ-0004771 as a Novel Diagnostic Biomarker of Colorectal Cancer
title_short Identification of Serum Exosomal hsa-circ-0004771 as a Novel Diagnostic Biomarker of Colorectal Cancer
title_sort identification of serum exosomal hsa-circ-0004771 as a novel diagnostic biomarker of colorectal cancer
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6838203/
https://www.ncbi.nlm.nih.gov/pubmed/31737058
http://dx.doi.org/10.3389/fgene.2019.01096
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