Cargando…

Reversible and Selective Interconversion of Hydrogen and Carbon Dioxide into Formate by a Semiartificial Formate Hydrogenlyase Mimic

[Image: see text] The biological formate hydrogenlyase (FHL) complex links a formate dehydrogenase (FDH) to a hydrogenase (H(2)ase) and produces H(2) and CO(2) from formate via mixed-acid fermentation in Escherichia coli. Here, we describe an electrochemical and a colloidal semiartificial FHL system...

Descripción completa

Detalles Bibliográficos
Autores principales: Sokol, Katarzyna P., Robinson, William E., Oliveira, Ana R., Zacarias, Sonia, Lee, Chong-Yong, Madden, Christopher, Bassegoda, Arnau, Hirst, Judy, Pereira, Inês A. C., Reisner, Erwin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2019
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6838786/
https://www.ncbi.nlm.nih.gov/pubmed/31638793
http://dx.doi.org/10.1021/jacs.9b09575
Descripción
Sumario:[Image: see text] The biological formate hydrogenlyase (FHL) complex links a formate dehydrogenase (FDH) to a hydrogenase (H(2)ase) and produces H(2) and CO(2) from formate via mixed-acid fermentation in Escherichia coli. Here, we describe an electrochemical and a colloidal semiartificial FHL system that consists of an FDH and a H(2)ase immobilized on conductive indium tin oxide (ITO) as an electron relay. These in vitro systems benefit from the efficient wiring of a highly active enzyme pair and allow for the reversible conversion of formate to H(2) and CO(2) under ambient temperature and pressure. The hybrid systems provide a template for the design of synthetic catalysts and surpass the FHL complex in vivo by storing and releasing H(2) on demand by interconverting CO(2)/H(2) and formate with minimal bias in either direction.