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Angular restriction fluorescence optical projection tomography to localize micrometastases in lymph nodes

Lymph node biopsy is a primary means of staging breast cancer, yet standard pathological techniques are time-consuming and typically sample less than 1% of the total node volume. A low-cost fluorescence optical projection tomography (OPT) protocol is demonstrated for rapid imaging of whole lymph nod...

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Autores principales: Torres, Veronica C., Li, Chengyue, He, Yusheng, Sinha, Lagnojita, Papavasiliou, Georgia, Sattar, Husain A., Brankov, Jovan G., Tichauer, Kenneth M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society of Photo-Optical Instrumentation Engineers 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6839382/
https://www.ncbi.nlm.nih.gov/pubmed/31705637
http://dx.doi.org/10.1117/1.JBO.24.11.110501
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author Torres, Veronica C.
Li, Chengyue
He, Yusheng
Sinha, Lagnojita
Papavasiliou, Georgia
Sattar, Husain A.
Brankov, Jovan G.
Tichauer, Kenneth M.
author_facet Torres, Veronica C.
Li, Chengyue
He, Yusheng
Sinha, Lagnojita
Papavasiliou, Georgia
Sattar, Husain A.
Brankov, Jovan G.
Tichauer, Kenneth M.
author_sort Torres, Veronica C.
collection PubMed
description Lymph node biopsy is a primary means of staging breast cancer, yet standard pathological techniques are time-consuming and typically sample less than 1% of the total node volume. A low-cost fluorescence optical projection tomography (OPT) protocol is demonstrated for rapid imaging of whole lymph nodes in three dimensions. The relatively low scattering properties of lymph node tissue can be leveraged to significantly improve spatial resolution of lymph node OPT by employing angular restriction of photon detection. It is demonstrated through porcine lymph node metastases models that simple filtered-backprojection reconstruction is sufficient to detect and localize [Formula: see text]-diameter metastases (the smallest clinically significant) in 1-cm-diameter lymph nodes.
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spelling pubmed-68393822020-02-06 Angular restriction fluorescence optical projection tomography to localize micrometastases in lymph nodes Torres, Veronica C. Li, Chengyue He, Yusheng Sinha, Lagnojita Papavasiliou, Georgia Sattar, Husain A. Brankov, Jovan G. Tichauer, Kenneth M. J Biomed Opt JBO Letters Lymph node biopsy is a primary means of staging breast cancer, yet standard pathological techniques are time-consuming and typically sample less than 1% of the total node volume. A low-cost fluorescence optical projection tomography (OPT) protocol is demonstrated for rapid imaging of whole lymph nodes in three dimensions. The relatively low scattering properties of lymph node tissue can be leveraged to significantly improve spatial resolution of lymph node OPT by employing angular restriction of photon detection. It is demonstrated through porcine lymph node metastases models that simple filtered-backprojection reconstruction is sufficient to detect and localize [Formula: see text]-diameter metastases (the smallest clinically significant) in 1-cm-diameter lymph nodes. Society of Photo-Optical Instrumentation Engineers 2019-11-08 2019-11 /pmc/articles/PMC6839382/ /pubmed/31705637 http://dx.doi.org/10.1117/1.JBO.24.11.110501 Text en © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
spellingShingle JBO Letters
Torres, Veronica C.
Li, Chengyue
He, Yusheng
Sinha, Lagnojita
Papavasiliou, Georgia
Sattar, Husain A.
Brankov, Jovan G.
Tichauer, Kenneth M.
Angular restriction fluorescence optical projection tomography to localize micrometastases in lymph nodes
title Angular restriction fluorescence optical projection tomography to localize micrometastases in lymph nodes
title_full Angular restriction fluorescence optical projection tomography to localize micrometastases in lymph nodes
title_fullStr Angular restriction fluorescence optical projection tomography to localize micrometastases in lymph nodes
title_full_unstemmed Angular restriction fluorescence optical projection tomography to localize micrometastases in lymph nodes
title_short Angular restriction fluorescence optical projection tomography to localize micrometastases in lymph nodes
title_sort angular restriction fluorescence optical projection tomography to localize micrometastases in lymph nodes
topic JBO Letters
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6839382/
https://www.ncbi.nlm.nih.gov/pubmed/31705637
http://dx.doi.org/10.1117/1.JBO.24.11.110501
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