Cargando…

Enhancement of antibiotics antimicrobial activity due to the silver nanoparticles impact on the cell membrane

The ability of microorganisms to generate resistance outcompetes with the generation of new and efficient antibiotics; therefore, it is critical to develop novel antibiotic agents and treatments to control bacterial infections. An alternative to this worldwide problem is the use of nanomaterials wit...

Descripción completa

Detalles Bibliográficos
Autores principales: Vazquez-Muñoz, R., Meza-Villezcas, A., Fournier, P. G. J., Soria-Castro, E., Juarez-Moreno, K., Gallego-Hernández, A. L., Bogdanchikova, N., Vazquez-Duhalt, R., Huerta-Saquero, A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6839893/
https://www.ncbi.nlm.nih.gov/pubmed/31703098
http://dx.doi.org/10.1371/journal.pone.0224904
_version_ 1783467507904937984
author Vazquez-Muñoz, R.
Meza-Villezcas, A.
Fournier, P. G. J.
Soria-Castro, E.
Juarez-Moreno, K.
Gallego-Hernández, A. L.
Bogdanchikova, N.
Vazquez-Duhalt, R.
Huerta-Saquero, A.
author_facet Vazquez-Muñoz, R.
Meza-Villezcas, A.
Fournier, P. G. J.
Soria-Castro, E.
Juarez-Moreno, K.
Gallego-Hernández, A. L.
Bogdanchikova, N.
Vazquez-Duhalt, R.
Huerta-Saquero, A.
author_sort Vazquez-Muñoz, R.
collection PubMed
description The ability of microorganisms to generate resistance outcompetes with the generation of new and efficient antibiotics; therefore, it is critical to develop novel antibiotic agents and treatments to control bacterial infections. An alternative to this worldwide problem is the use of nanomaterials with antimicrobial properties. Silver nanoparticles (AgNPs) have been extensively studied due to their antimicrobial effect in different organisms. In this work, the synergistic antimicrobial effect of AgNPs and conventional antibiotics was assessed in Gram-positive and Gram-negative bacteria. AgNPs minimal inhibitory concentration was 10–12 μg mL(-1) in all bacterial strains tested, regardless of their different susceptibility against antibiotics. Interestingly, a synergistic antimicrobial effect was observed when combining AgNPs and kanamycin according to the fractional inhibitory concentration index, FICI: <0.5), an additive effect by combining AgNPs and chloramphenicol (FICI: 0.5 to 1), whereas no effect was found with AgNPs and β-lactam antibiotics combinations. Flow cytometry and TEM analysis showed that sublethal concentrations of AgNPs (6–7 μg mL(-1)) altered the bacterial membrane potential and caused ultrastructural damage, increasing the cell membrane permeability. No chemical interactions between AgNPs and antibiotics were detected. We propose an experimental supported mechanism of action by which combinatorial effect of antimicrobials drives synergy depending on their specific target, facilitated by membrane alterations generated by AgNPs. Our results provide a deeper understanding about the synergistic mechanism of AgNPs and antibiotics, aiming to combat antimicrobial infections efficiently, especially those by multi-drug resistant microorganisms, in order to mitigate the current crisis due to antibiotic resistance.
format Online
Article
Text
id pubmed-6839893
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-68398932019-11-15 Enhancement of antibiotics antimicrobial activity due to the silver nanoparticles impact on the cell membrane Vazquez-Muñoz, R. Meza-Villezcas, A. Fournier, P. G. J. Soria-Castro, E. Juarez-Moreno, K. Gallego-Hernández, A. L. Bogdanchikova, N. Vazquez-Duhalt, R. Huerta-Saquero, A. PLoS One Research Article The ability of microorganisms to generate resistance outcompetes with the generation of new and efficient antibiotics; therefore, it is critical to develop novel antibiotic agents and treatments to control bacterial infections. An alternative to this worldwide problem is the use of nanomaterials with antimicrobial properties. Silver nanoparticles (AgNPs) have been extensively studied due to their antimicrobial effect in different organisms. In this work, the synergistic antimicrobial effect of AgNPs and conventional antibiotics was assessed in Gram-positive and Gram-negative bacteria. AgNPs minimal inhibitory concentration was 10–12 μg mL(-1) in all bacterial strains tested, regardless of their different susceptibility against antibiotics. Interestingly, a synergistic antimicrobial effect was observed when combining AgNPs and kanamycin according to the fractional inhibitory concentration index, FICI: <0.5), an additive effect by combining AgNPs and chloramphenicol (FICI: 0.5 to 1), whereas no effect was found with AgNPs and β-lactam antibiotics combinations. Flow cytometry and TEM analysis showed that sublethal concentrations of AgNPs (6–7 μg mL(-1)) altered the bacterial membrane potential and caused ultrastructural damage, increasing the cell membrane permeability. No chemical interactions between AgNPs and antibiotics were detected. We propose an experimental supported mechanism of action by which combinatorial effect of antimicrobials drives synergy depending on their specific target, facilitated by membrane alterations generated by AgNPs. Our results provide a deeper understanding about the synergistic mechanism of AgNPs and antibiotics, aiming to combat antimicrobial infections efficiently, especially those by multi-drug resistant microorganisms, in order to mitigate the current crisis due to antibiotic resistance. Public Library of Science 2019-11-08 /pmc/articles/PMC6839893/ /pubmed/31703098 http://dx.doi.org/10.1371/journal.pone.0224904 Text en © 2019 Vazquez-Muñoz et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Vazquez-Muñoz, R.
Meza-Villezcas, A.
Fournier, P. G. J.
Soria-Castro, E.
Juarez-Moreno, K.
Gallego-Hernández, A. L.
Bogdanchikova, N.
Vazquez-Duhalt, R.
Huerta-Saquero, A.
Enhancement of antibiotics antimicrobial activity due to the silver nanoparticles impact on the cell membrane
title Enhancement of antibiotics antimicrobial activity due to the silver nanoparticles impact on the cell membrane
title_full Enhancement of antibiotics antimicrobial activity due to the silver nanoparticles impact on the cell membrane
title_fullStr Enhancement of antibiotics antimicrobial activity due to the silver nanoparticles impact on the cell membrane
title_full_unstemmed Enhancement of antibiotics antimicrobial activity due to the silver nanoparticles impact on the cell membrane
title_short Enhancement of antibiotics antimicrobial activity due to the silver nanoparticles impact on the cell membrane
title_sort enhancement of antibiotics antimicrobial activity due to the silver nanoparticles impact on the cell membrane
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6839893/
https://www.ncbi.nlm.nih.gov/pubmed/31703098
http://dx.doi.org/10.1371/journal.pone.0224904
work_keys_str_mv AT vazquezmunozr enhancementofantibioticsantimicrobialactivityduetothesilvernanoparticlesimpactonthecellmembrane
AT mezavillezcasa enhancementofantibioticsantimicrobialactivityduetothesilvernanoparticlesimpactonthecellmembrane
AT fournierpgj enhancementofantibioticsantimicrobialactivityduetothesilvernanoparticlesimpactonthecellmembrane
AT soriacastroe enhancementofantibioticsantimicrobialactivityduetothesilvernanoparticlesimpactonthecellmembrane
AT juarezmorenok enhancementofantibioticsantimicrobialactivityduetothesilvernanoparticlesimpactonthecellmembrane
AT gallegohernandezal enhancementofantibioticsantimicrobialactivityduetothesilvernanoparticlesimpactonthecellmembrane
AT bogdanchikovan enhancementofantibioticsantimicrobialactivityduetothesilvernanoparticlesimpactonthecellmembrane
AT vazquezduhaltr enhancementofantibioticsantimicrobialactivityduetothesilvernanoparticlesimpactonthecellmembrane
AT huertasaqueroa enhancementofantibioticsantimicrobialactivityduetothesilvernanoparticlesimpactonthecellmembrane