Cryo-EM structures reveal coordinated domain motions that govern DNA cleavage by Cas9

The RNA-guided Cas9 endonuclease from Streptococcus pyogenes is a single turnover enzyme that displays a stable product state after double-stranded DNA cleavage. Here, we present cryo-EM structures of pre-catalytic, post-catalytic, and product states of the active Cas9-sgRNA-DNA complex in the prese...

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Detalles Bibliográficos
Autores principales: Zhu, Xing, Clarke, Ryan, Puppala, Anupama K., Chittori, Sagar, Merk, Alan, Merrill, Bradley J., Simonović, Miljan, Subramaniam, Sriram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6842131/
https://www.ncbi.nlm.nih.gov/pubmed/31285607
http://dx.doi.org/10.1038/s41594-019-0258-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6842131/
https://www.ncbi.nlm.nih.gov/pubmed/31285607
http://dx.doi.org/10.1038/s41594-019-0258-2

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