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Cryo-EM structures reveal coordinated domain motions that govern DNA cleavage by Cas9
The RNA-guided Cas9 endonuclease from Streptococcus pyogenes is a single turnover enzyme that displays a stable product state after double-stranded DNA cleavage. Here, we present cryo-EM structures of pre-catalytic, post-catalytic, and product states of the active Cas9-sgRNA-DNA complex in the prese...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6842131/ https://www.ncbi.nlm.nih.gov/pubmed/31285607 http://dx.doi.org/10.1038/s41594-019-0258-2 |