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The Plant NF-Y DNA Matrix In Vitro and In Vivo
Nuclear Factor Y (NF-Y) is an evolutionarily conserved trimer formed by a Histone-Fold Domain (HFD) heterodimeric module shared by core histones, and the sequence-specific NF-YA subunit. In plants, the genes encoding each of the three subunits have expanded in number, giving rise to hundreds of pote...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6843132/ https://www.ncbi.nlm.nih.gov/pubmed/31658622 http://dx.doi.org/10.3390/plants8100406 |
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author | Gnesutta, Nerina Chiara, Matteo Bernardini, Andrea Balestra, Matteo Horner, David S. Mantovani, Roberto |
author_facet | Gnesutta, Nerina Chiara, Matteo Bernardini, Andrea Balestra, Matteo Horner, David S. Mantovani, Roberto |
author_sort | Gnesutta, Nerina |
collection | PubMed |
description | Nuclear Factor Y (NF-Y) is an evolutionarily conserved trimer formed by a Histone-Fold Domain (HFD) heterodimeric module shared by core histones, and the sequence-specific NF-YA subunit. In plants, the genes encoding each of the three subunits have expanded in number, giving rise to hundreds of potential trimers. While in mammals NF-Y binds a well-characterized motif, with a defined matrix centered on the CCAAT box, the specificity of the plant trimers has yet to be determined. Here we report that Arabidopsis thaliana NF-Y trimeric complexes, containing two different NF-YA subunits, bind DNA in vitro with similar affinities. We assayed precisely sequence-specificity by saturation mutagenesis, and analyzed genomic DNA sites bound in vivo by selected HFDs. The plant NF-Y CCAAT matrix is different in nucleotides flanking CCAAT with respect to the mammalian matrix, in vitro and in vivo. Our data point to flexible DNA-binding rules by plant NF-Ys, serving the scope of adapting to a diverse audience of genomic motifs. |
format | Online Article Text |
id | pubmed-6843132 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68431322019-11-25 The Plant NF-Y DNA Matrix In Vitro and In Vivo Gnesutta, Nerina Chiara, Matteo Bernardini, Andrea Balestra, Matteo Horner, David S. Mantovani, Roberto Plants (Basel) Article Nuclear Factor Y (NF-Y) is an evolutionarily conserved trimer formed by a Histone-Fold Domain (HFD) heterodimeric module shared by core histones, and the sequence-specific NF-YA subunit. In plants, the genes encoding each of the three subunits have expanded in number, giving rise to hundreds of potential trimers. While in mammals NF-Y binds a well-characterized motif, with a defined matrix centered on the CCAAT box, the specificity of the plant trimers has yet to be determined. Here we report that Arabidopsis thaliana NF-Y trimeric complexes, containing two different NF-YA subunits, bind DNA in vitro with similar affinities. We assayed precisely sequence-specificity by saturation mutagenesis, and analyzed genomic DNA sites bound in vivo by selected HFDs. The plant NF-Y CCAAT matrix is different in nucleotides flanking CCAAT with respect to the mammalian matrix, in vitro and in vivo. Our data point to flexible DNA-binding rules by plant NF-Ys, serving the scope of adapting to a diverse audience of genomic motifs. MDPI 2019-10-10 /pmc/articles/PMC6843132/ /pubmed/31658622 http://dx.doi.org/10.3390/plants8100406 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gnesutta, Nerina Chiara, Matteo Bernardini, Andrea Balestra, Matteo Horner, David S. Mantovani, Roberto The Plant NF-Y DNA Matrix In Vitro and In Vivo |
title | The Plant NF-Y DNA Matrix In Vitro and In Vivo |
title_full | The Plant NF-Y DNA Matrix In Vitro and In Vivo |
title_fullStr | The Plant NF-Y DNA Matrix In Vitro and In Vivo |
title_full_unstemmed | The Plant NF-Y DNA Matrix In Vitro and In Vivo |
title_short | The Plant NF-Y DNA Matrix In Vitro and In Vivo |
title_sort | plant nf-y dna matrix in vitro and in vivo |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6843132/ https://www.ncbi.nlm.nih.gov/pubmed/31658622 http://dx.doi.org/10.3390/plants8100406 |
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