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One Small RNA of Fusarium graminearum Targets and Silences CEBiP Gene in Common Wheat

The pathogenic fungus Fusarium graminearum (F. graminearum), causing Fusarium head blight (FHB) or scab, is one of the most important cereal killers worldwide, exerting great economic and agronomic losses on global grain production. To repress pathogen invasion, plants have evolved a sophisticated i...

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Autores principales: Jian, Jiao, Liang, Xu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6843203/
https://www.ncbi.nlm.nih.gov/pubmed/31600909
http://dx.doi.org/10.3390/microorganisms7100425
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author Jian, Jiao
Liang, Xu
author_facet Jian, Jiao
Liang, Xu
author_sort Jian, Jiao
collection PubMed
description The pathogenic fungus Fusarium graminearum (F. graminearum), causing Fusarium head blight (FHB) or scab, is one of the most important cereal killers worldwide, exerting great economic and agronomic losses on global grain production. To repress pathogen invasion, plants have evolved a sophisticated innate immunity system for pathogen recognition and defense activation. Simultaneously, pathogens continue to evolve more effective means of invasion to conquer plant resistance systems. In the process of co-evolution of plants and pathogens, several small RNAs (sRNAs) have been proved in regulating plant immune response and plant-microbial interaction. In this study, we report that a F. graminearum sRNA (Fg-sRNA1) can suppress wheat defense response by targeting and silencing a resistance-related gene, which codes a Chitin Elicitor Binding Protein (TaCEBiP). Transcriptional level evidence indicates that Fg-sRNA1 can target TaCEBiP mRNA and trigger silencing of TaCEBiP in vivo, and in Nicotiana benthamiana (N. benthamiana) plants, Western blotting experiments and YFP Fluorescence observation proofs show that Fg-sRNA1 can suppress the accumulation of protein coding by TaCEBiP gene in vitro. F. graminearum PH-1 strain displays a weakening ability to invasion when Barley stripe mosaic virus (BSMV) vector induces effective silencing Fg-sRNA1 in PH-1 infected wheat plants. Taken together, our results suggest that a small RNA from F. graminearum can target and silence the wheat TaCEBiP gene to enhance invasion of F. graminearum.
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spelling pubmed-68432032019-11-25 One Small RNA of Fusarium graminearum Targets and Silences CEBiP Gene in Common Wheat Jian, Jiao Liang, Xu Microorganisms Article The pathogenic fungus Fusarium graminearum (F. graminearum), causing Fusarium head blight (FHB) or scab, is one of the most important cereal killers worldwide, exerting great economic and agronomic losses on global grain production. To repress pathogen invasion, plants have evolved a sophisticated innate immunity system for pathogen recognition and defense activation. Simultaneously, pathogens continue to evolve more effective means of invasion to conquer plant resistance systems. In the process of co-evolution of plants and pathogens, several small RNAs (sRNAs) have been proved in regulating plant immune response and plant-microbial interaction. In this study, we report that a F. graminearum sRNA (Fg-sRNA1) can suppress wheat defense response by targeting and silencing a resistance-related gene, which codes a Chitin Elicitor Binding Protein (TaCEBiP). Transcriptional level evidence indicates that Fg-sRNA1 can target TaCEBiP mRNA and trigger silencing of TaCEBiP in vivo, and in Nicotiana benthamiana (N. benthamiana) plants, Western blotting experiments and YFP Fluorescence observation proofs show that Fg-sRNA1 can suppress the accumulation of protein coding by TaCEBiP gene in vitro. F. graminearum PH-1 strain displays a weakening ability to invasion when Barley stripe mosaic virus (BSMV) vector induces effective silencing Fg-sRNA1 in PH-1 infected wheat plants. Taken together, our results suggest that a small RNA from F. graminearum can target and silence the wheat TaCEBiP gene to enhance invasion of F. graminearum. MDPI 2019-10-09 /pmc/articles/PMC6843203/ /pubmed/31600909 http://dx.doi.org/10.3390/microorganisms7100425 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jian, Jiao
Liang, Xu
One Small RNA of Fusarium graminearum Targets and Silences CEBiP Gene in Common Wheat
title One Small RNA of Fusarium graminearum Targets and Silences CEBiP Gene in Common Wheat
title_full One Small RNA of Fusarium graminearum Targets and Silences CEBiP Gene in Common Wheat
title_fullStr One Small RNA of Fusarium graminearum Targets and Silences CEBiP Gene in Common Wheat
title_full_unstemmed One Small RNA of Fusarium graminearum Targets and Silences CEBiP Gene in Common Wheat
title_short One Small RNA of Fusarium graminearum Targets and Silences CEBiP Gene in Common Wheat
title_sort one small rna of fusarium graminearum targets and silences cebip gene in common wheat
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6843203/
https://www.ncbi.nlm.nih.gov/pubmed/31600909
http://dx.doi.org/10.3390/microorganisms7100425
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