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Taking Advantage of Bacterial Adaptation in Order to Optimize Industrial Production of Dry Propionibacterium freudenreichii
Propionibacterium freudenreichii is a beneficial bacterium, used both as a probiotic and as a cheese starter. Large-scale production of P. freudenreichii is required to meet growing consumers’ demand. Production, drying and storage must be optimized, in order to guarantee high P. freudenreichii viab...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6843336/ https://www.ncbi.nlm.nih.gov/pubmed/31652621 http://dx.doi.org/10.3390/microorganisms7100477 |
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author | Gaucher, Floriane Gagnaire, Valérie Rabah, Houem Maillard, Marie-Bernadette Bonnassie, Sylvie Pottier, Sandrine Marchand, Pierre Jan, Gwénaël Blanc, Philippe Jeantet, Romain |
author_facet | Gaucher, Floriane Gagnaire, Valérie Rabah, Houem Maillard, Marie-Bernadette Bonnassie, Sylvie Pottier, Sandrine Marchand, Pierre Jan, Gwénaël Blanc, Philippe Jeantet, Romain |
author_sort | Gaucher, Floriane |
collection | PubMed |
description | Propionibacterium freudenreichii is a beneficial bacterium, used both as a probiotic and as a cheese starter. Large-scale production of P. freudenreichii is required to meet growing consumers’ demand. Production, drying and storage must be optimized, in order to guarantee high P. freudenreichii viability within powders. Compared to freeze-drying, spray drying constitutes the most productive and efficient, yet the most stressful process, imposing severe oxidative and thermal constraints. The aim of our study was to provide the tools in order to optimize the industrial production of dry P. freudenreichii. Bacterial adaptation is a well-known protective mechanism and may be used to improve bacterial tolerance towards technological stresses. However, the choice of bacterial adaptation type must consider industrial constraints. In this study, we combined (i) modulation of the growth medium composition, (ii) heat-adaptation, and (iii) osmoadaptation, in order to increase P. freudenreichii tolerance towards technological stresses, including thermal and oxidative constraints, using an experimental design. We further investigated optimal growth and adaptation conditions, by monitoring intracellular compatible solutes accumulation. Glucose addition, coupled to heat-adaptation, triggered accumulation of trehalose and of glycine betaine, which further provided high tolerance towards spray drying and storage. This work opens new perspectives for high quality and fast production of live propionibacteria at the industrial scale. |
format | Online Article Text |
id | pubmed-6843336 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68433362019-11-25 Taking Advantage of Bacterial Adaptation in Order to Optimize Industrial Production of Dry Propionibacterium freudenreichii Gaucher, Floriane Gagnaire, Valérie Rabah, Houem Maillard, Marie-Bernadette Bonnassie, Sylvie Pottier, Sandrine Marchand, Pierre Jan, Gwénaël Blanc, Philippe Jeantet, Romain Microorganisms Article Propionibacterium freudenreichii is a beneficial bacterium, used both as a probiotic and as a cheese starter. Large-scale production of P. freudenreichii is required to meet growing consumers’ demand. Production, drying and storage must be optimized, in order to guarantee high P. freudenreichii viability within powders. Compared to freeze-drying, spray drying constitutes the most productive and efficient, yet the most stressful process, imposing severe oxidative and thermal constraints. The aim of our study was to provide the tools in order to optimize the industrial production of dry P. freudenreichii. Bacterial adaptation is a well-known protective mechanism and may be used to improve bacterial tolerance towards technological stresses. However, the choice of bacterial adaptation type must consider industrial constraints. In this study, we combined (i) modulation of the growth medium composition, (ii) heat-adaptation, and (iii) osmoadaptation, in order to increase P. freudenreichii tolerance towards technological stresses, including thermal and oxidative constraints, using an experimental design. We further investigated optimal growth and adaptation conditions, by monitoring intracellular compatible solutes accumulation. Glucose addition, coupled to heat-adaptation, triggered accumulation of trehalose and of glycine betaine, which further provided high tolerance towards spray drying and storage. This work opens new perspectives for high quality and fast production of live propionibacteria at the industrial scale. MDPI 2019-10-22 /pmc/articles/PMC6843336/ /pubmed/31652621 http://dx.doi.org/10.3390/microorganisms7100477 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gaucher, Floriane Gagnaire, Valérie Rabah, Houem Maillard, Marie-Bernadette Bonnassie, Sylvie Pottier, Sandrine Marchand, Pierre Jan, Gwénaël Blanc, Philippe Jeantet, Romain Taking Advantage of Bacterial Adaptation in Order to Optimize Industrial Production of Dry Propionibacterium freudenreichii |
title | Taking Advantage of Bacterial Adaptation in Order to Optimize Industrial Production of Dry Propionibacterium freudenreichii |
title_full | Taking Advantage of Bacterial Adaptation in Order to Optimize Industrial Production of Dry Propionibacterium freudenreichii |
title_fullStr | Taking Advantage of Bacterial Adaptation in Order to Optimize Industrial Production of Dry Propionibacterium freudenreichii |
title_full_unstemmed | Taking Advantage of Bacterial Adaptation in Order to Optimize Industrial Production of Dry Propionibacterium freudenreichii |
title_short | Taking Advantage of Bacterial Adaptation in Order to Optimize Industrial Production of Dry Propionibacterium freudenreichii |
title_sort | taking advantage of bacterial adaptation in order to optimize industrial production of dry propionibacterium freudenreichii |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6843336/ https://www.ncbi.nlm.nih.gov/pubmed/31652621 http://dx.doi.org/10.3390/microorganisms7100477 |
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