Cargando…

Taking Advantage of Bacterial Adaptation in Order to Optimize Industrial Production of Dry Propionibacterium freudenreichii

Propionibacterium freudenreichii is a beneficial bacterium, used both as a probiotic and as a cheese starter. Large-scale production of P. freudenreichii is required to meet growing consumers’ demand. Production, drying and storage must be optimized, in order to guarantee high P. freudenreichii viab...

Descripción completa

Detalles Bibliográficos
Autores principales: Gaucher, Floriane, Gagnaire, Valérie, Rabah, Houem, Maillard, Marie-Bernadette, Bonnassie, Sylvie, Pottier, Sandrine, Marchand, Pierre, Jan, Gwénaël, Blanc, Philippe, Jeantet, Romain
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6843336/
https://www.ncbi.nlm.nih.gov/pubmed/31652621
http://dx.doi.org/10.3390/microorganisms7100477
_version_ 1783468191307005952
author Gaucher, Floriane
Gagnaire, Valérie
Rabah, Houem
Maillard, Marie-Bernadette
Bonnassie, Sylvie
Pottier, Sandrine
Marchand, Pierre
Jan, Gwénaël
Blanc, Philippe
Jeantet, Romain
author_facet Gaucher, Floriane
Gagnaire, Valérie
Rabah, Houem
Maillard, Marie-Bernadette
Bonnassie, Sylvie
Pottier, Sandrine
Marchand, Pierre
Jan, Gwénaël
Blanc, Philippe
Jeantet, Romain
author_sort Gaucher, Floriane
collection PubMed
description Propionibacterium freudenreichii is a beneficial bacterium, used both as a probiotic and as a cheese starter. Large-scale production of P. freudenreichii is required to meet growing consumers’ demand. Production, drying and storage must be optimized, in order to guarantee high P. freudenreichii viability within powders. Compared to freeze-drying, spray drying constitutes the most productive and efficient, yet the most stressful process, imposing severe oxidative and thermal constraints. The aim of our study was to provide the tools in order to optimize the industrial production of dry P. freudenreichii. Bacterial adaptation is a well-known protective mechanism and may be used to improve bacterial tolerance towards technological stresses. However, the choice of bacterial adaptation type must consider industrial constraints. In this study, we combined (i) modulation of the growth medium composition, (ii) heat-adaptation, and (iii) osmoadaptation, in order to increase P. freudenreichii tolerance towards technological stresses, including thermal and oxidative constraints, using an experimental design. We further investigated optimal growth and adaptation conditions, by monitoring intracellular compatible solutes accumulation. Glucose addition, coupled to heat-adaptation, triggered accumulation of trehalose and of glycine betaine, which further provided high tolerance towards spray drying and storage. This work opens new perspectives for high quality and fast production of live propionibacteria at the industrial scale.
format Online
Article
Text
id pubmed-6843336
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-68433362019-11-25 Taking Advantage of Bacterial Adaptation in Order to Optimize Industrial Production of Dry Propionibacterium freudenreichii Gaucher, Floriane Gagnaire, Valérie Rabah, Houem Maillard, Marie-Bernadette Bonnassie, Sylvie Pottier, Sandrine Marchand, Pierre Jan, Gwénaël Blanc, Philippe Jeantet, Romain Microorganisms Article Propionibacterium freudenreichii is a beneficial bacterium, used both as a probiotic and as a cheese starter. Large-scale production of P. freudenreichii is required to meet growing consumers’ demand. Production, drying and storage must be optimized, in order to guarantee high P. freudenreichii viability within powders. Compared to freeze-drying, spray drying constitutes the most productive and efficient, yet the most stressful process, imposing severe oxidative and thermal constraints. The aim of our study was to provide the tools in order to optimize the industrial production of dry P. freudenreichii. Bacterial adaptation is a well-known protective mechanism and may be used to improve bacterial tolerance towards technological stresses. However, the choice of bacterial adaptation type must consider industrial constraints. In this study, we combined (i) modulation of the growth medium composition, (ii) heat-adaptation, and (iii) osmoadaptation, in order to increase P. freudenreichii tolerance towards technological stresses, including thermal and oxidative constraints, using an experimental design. We further investigated optimal growth and adaptation conditions, by monitoring intracellular compatible solutes accumulation. Glucose addition, coupled to heat-adaptation, triggered accumulation of trehalose and of glycine betaine, which further provided high tolerance towards spray drying and storage. This work opens new perspectives for high quality and fast production of live propionibacteria at the industrial scale. MDPI 2019-10-22 /pmc/articles/PMC6843336/ /pubmed/31652621 http://dx.doi.org/10.3390/microorganisms7100477 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gaucher, Floriane
Gagnaire, Valérie
Rabah, Houem
Maillard, Marie-Bernadette
Bonnassie, Sylvie
Pottier, Sandrine
Marchand, Pierre
Jan, Gwénaël
Blanc, Philippe
Jeantet, Romain
Taking Advantage of Bacterial Adaptation in Order to Optimize Industrial Production of Dry Propionibacterium freudenreichii
title Taking Advantage of Bacterial Adaptation in Order to Optimize Industrial Production of Dry Propionibacterium freudenreichii
title_full Taking Advantage of Bacterial Adaptation in Order to Optimize Industrial Production of Dry Propionibacterium freudenreichii
title_fullStr Taking Advantage of Bacterial Adaptation in Order to Optimize Industrial Production of Dry Propionibacterium freudenreichii
title_full_unstemmed Taking Advantage of Bacterial Adaptation in Order to Optimize Industrial Production of Dry Propionibacterium freudenreichii
title_short Taking Advantage of Bacterial Adaptation in Order to Optimize Industrial Production of Dry Propionibacterium freudenreichii
title_sort taking advantage of bacterial adaptation in order to optimize industrial production of dry propionibacterium freudenreichii
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6843336/
https://www.ncbi.nlm.nih.gov/pubmed/31652621
http://dx.doi.org/10.3390/microorganisms7100477
work_keys_str_mv AT gaucherfloriane takingadvantageofbacterialadaptationinordertooptimizeindustrialproductionofdrypropionibacteriumfreudenreichii
AT gagnairevalerie takingadvantageofbacterialadaptationinordertooptimizeindustrialproductionofdrypropionibacteriumfreudenreichii
AT rabahhouem takingadvantageofbacterialadaptationinordertooptimizeindustrialproductionofdrypropionibacteriumfreudenreichii
AT maillardmariebernadette takingadvantageofbacterialadaptationinordertooptimizeindustrialproductionofdrypropionibacteriumfreudenreichii
AT bonnassiesylvie takingadvantageofbacterialadaptationinordertooptimizeindustrialproductionofdrypropionibacteriumfreudenreichii
AT pottiersandrine takingadvantageofbacterialadaptationinordertooptimizeindustrialproductionofdrypropionibacteriumfreudenreichii
AT marchandpierre takingadvantageofbacterialadaptationinordertooptimizeindustrialproductionofdrypropionibacteriumfreudenreichii
AT jangwenael takingadvantageofbacterialadaptationinordertooptimizeindustrialproductionofdrypropionibacteriumfreudenreichii
AT blancphilippe takingadvantageofbacterialadaptationinordertooptimizeindustrialproductionofdrypropionibacteriumfreudenreichii
AT jeantetromain takingadvantageofbacterialadaptationinordertooptimizeindustrialproductionofdrypropionibacteriumfreudenreichii