Cargando…
Intestinal Microbiota of Grass Carp Fed Faba Beans: A Comparative Study
Many reports of the intestinal microbiota of grass carp have addressed the microbial response to diet or starvation or the effect of microbes on metabolism; however, the intestinal microbiota of crisp grass carp has yet to be elucidated. Moreover, the specific bacteria that play a role in the crispi...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6843481/ https://www.ncbi.nlm.nih.gov/pubmed/31627338 http://dx.doi.org/10.3390/microorganisms7100465 |
_version_ | 1783468225750630400 |
---|---|
author | Zhou, Lei Lin, Ke-tao Gan, Lian Sun, Ji-jia Guo, Chang-jun Liu, Li Huang, Xian-de |
author_facet | Zhou, Lei Lin, Ke-tao Gan, Lian Sun, Ji-jia Guo, Chang-jun Liu, Li Huang, Xian-de |
author_sort | Zhou, Lei |
collection | PubMed |
description | Many reports of the intestinal microbiota of grass carp have addressed the microbial response to diet or starvation or the effect of microbes on metabolism; however, the intestinal microbiota of crisp grass carp has yet to be elucidated. Moreover, the specific bacteria that play a role in the crispiness of grass carp fed faba beans have not been elucidated. In the present study, 16S sequencing was carried out to compare the intestinal microbiota in the fore-, mid- and hind-intestine segments of grass carp following feeding with either faba beans or formula feed. Our results showed that (1) the hind-intestine presented significant differences in diversity relative to the fore- or midintestine and (2) faba beans significantly increased the diversity of intestinal microbiota, changed the intestinal microbiota structure (Fusobacteria was reduced from 64.26% to 18.24%, while Proteobacteria was significantly increased from 17.75% to 51.99%), and decreased the metabolism of energy, cofactors and vitamins in grass carp. Furthermore, at the genus and species levels, Acinetobacter accounted for 15.09% of the microbiota, and Acinetobacter johnsonii and Acinetobacter radioresistens constituted 3.41% and 2.99%, respectively, which indicated that Acinetobacter of the family Moraxellaceae contributed to changes in the intestinal microbiota structure and could be used as a potential biomarker. These results may provide clues at the intestinal microbiota level to understanding the mechanism underlying the crispiness of grass carp fed faba beans. |
format | Online Article Text |
id | pubmed-6843481 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68434812019-11-25 Intestinal Microbiota of Grass Carp Fed Faba Beans: A Comparative Study Zhou, Lei Lin, Ke-tao Gan, Lian Sun, Ji-jia Guo, Chang-jun Liu, Li Huang, Xian-de Microorganisms Article Many reports of the intestinal microbiota of grass carp have addressed the microbial response to diet or starvation or the effect of microbes on metabolism; however, the intestinal microbiota of crisp grass carp has yet to be elucidated. Moreover, the specific bacteria that play a role in the crispiness of grass carp fed faba beans have not been elucidated. In the present study, 16S sequencing was carried out to compare the intestinal microbiota in the fore-, mid- and hind-intestine segments of grass carp following feeding with either faba beans or formula feed. Our results showed that (1) the hind-intestine presented significant differences in diversity relative to the fore- or midintestine and (2) faba beans significantly increased the diversity of intestinal microbiota, changed the intestinal microbiota structure (Fusobacteria was reduced from 64.26% to 18.24%, while Proteobacteria was significantly increased from 17.75% to 51.99%), and decreased the metabolism of energy, cofactors and vitamins in grass carp. Furthermore, at the genus and species levels, Acinetobacter accounted for 15.09% of the microbiota, and Acinetobacter johnsonii and Acinetobacter radioresistens constituted 3.41% and 2.99%, respectively, which indicated that Acinetobacter of the family Moraxellaceae contributed to changes in the intestinal microbiota structure and could be used as a potential biomarker. These results may provide clues at the intestinal microbiota level to understanding the mechanism underlying the crispiness of grass carp fed faba beans. MDPI 2019-10-17 /pmc/articles/PMC6843481/ /pubmed/31627338 http://dx.doi.org/10.3390/microorganisms7100465 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhou, Lei Lin, Ke-tao Gan, Lian Sun, Ji-jia Guo, Chang-jun Liu, Li Huang, Xian-de Intestinal Microbiota of Grass Carp Fed Faba Beans: A Comparative Study |
title | Intestinal Microbiota of Grass Carp Fed Faba Beans: A Comparative Study |
title_full | Intestinal Microbiota of Grass Carp Fed Faba Beans: A Comparative Study |
title_fullStr | Intestinal Microbiota of Grass Carp Fed Faba Beans: A Comparative Study |
title_full_unstemmed | Intestinal Microbiota of Grass Carp Fed Faba Beans: A Comparative Study |
title_short | Intestinal Microbiota of Grass Carp Fed Faba Beans: A Comparative Study |
title_sort | intestinal microbiota of grass carp fed faba beans: a comparative study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6843481/ https://www.ncbi.nlm.nih.gov/pubmed/31627338 http://dx.doi.org/10.3390/microorganisms7100465 |
work_keys_str_mv | AT zhoulei intestinalmicrobiotaofgrasscarpfedfababeansacomparativestudy AT linketao intestinalmicrobiotaofgrasscarpfedfababeansacomparativestudy AT ganlian intestinalmicrobiotaofgrasscarpfedfababeansacomparativestudy AT sunjijia intestinalmicrobiotaofgrasscarpfedfababeansacomparativestudy AT guochangjun intestinalmicrobiotaofgrasscarpfedfababeansacomparativestudy AT liuli intestinalmicrobiotaofgrasscarpfedfababeansacomparativestudy AT huangxiande intestinalmicrobiotaofgrasscarpfedfababeansacomparativestudy |