Cargando…

IL28B Genetic Variations in Patients with Recurrent Herpes Simplex Keratitis

Background and objectives: Recurrent herpes simplex keratitis (HSK) is the most common cause of corneal blindness in the developed world. A relationship between host gene polymorphisms and the recurrence of herpes simplex virus (HSV) infection has previously been proposed. Thus, the aim of this stud...

Descripción completa

Detalles Bibliográficos
Autores principales: Borivoje, Savić, Svetlana, Stanojlović, Milan, Hadži-Milić, Nela, Đonović, Olivera, Milošević-Đorđević, Filip, Milisavljević, Milenko, Stojković, Srbislav, Pajić
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6843532/
https://www.ncbi.nlm.nih.gov/pubmed/31561422
http://dx.doi.org/10.3390/medicina55100642
_version_ 1783468237823934464
author Borivoje, Savić
Svetlana, Stanojlović
Milan, Hadži-Milić
Nela, Đonović
Olivera, Milošević-Đorđević
Filip, Milisavljević
Milenko, Stojković
Srbislav, Pajić
author_facet Borivoje, Savić
Svetlana, Stanojlović
Milan, Hadži-Milić
Nela, Đonović
Olivera, Milošević-Đorđević
Filip, Milisavljević
Milenko, Stojković
Srbislav, Pajić
author_sort Borivoje, Savić
collection PubMed
description Background and objectives: Recurrent herpes simplex keratitis (HSK) is the most common cause of corneal blindness in the developed world. A relationship between host gene polymorphisms and the recurrence of herpes simplex virus (HSV) infection has previously been proposed. Thus, the aim of this study was to investigate a potential association between the IL28B host genotype and recurrent HSK. Materials and Methods: Eighty patients older than 18 years of age of both genders with a history of recurrent herpes simplex labialis (HSL) were considered for inclusion. Seventy-five of these patients were found to be seropositive for HSV-1 and were subsequently enrolled in the study. Twenty-four of the enrolled patients also had a history of recurrent HSK associated with severe corneal scarring and visual acuity deterioration. Total DNA was isolated from whole blood samples. A single-nucleotide polymorphism (SNP) rs12979860 near the IL28B gene on chromosome 19 was genotyped. Results: A significant association was observed between recurrent HSK and two SNPs of the IL28B genotype (CCrs12979860 and CTrs12979860, p < 0.01). The variation CCrs12979860 showed a significantly greater association with HSK (16 out of 26 patients) compared with CTrs12979860 (8 out of 34 patients). Conclusion: Seropositive individuals with a history of recurrent HSK are likely to have the CC IL28B genotype. This genotype may be related to incomplete control of the infection and more frequent periodical viral shedding along the first nerve branch of the trigeminal ganglion, which clinically manifests as recurrent herpes keratitis. The clinical manifestation of recurrent HSV-1 infection seems to be influenced by polymorphism of the IL28B genotype.
format Online
Article
Text
id pubmed-6843532
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-68435322019-11-25 IL28B Genetic Variations in Patients with Recurrent Herpes Simplex Keratitis Borivoje, Savić Svetlana, Stanojlović Milan, Hadži-Milić Nela, Đonović Olivera, Milošević-Đorđević Filip, Milisavljević Milenko, Stojković Srbislav, Pajić Medicina (Kaunas) Article Background and objectives: Recurrent herpes simplex keratitis (HSK) is the most common cause of corneal blindness in the developed world. A relationship between host gene polymorphisms and the recurrence of herpes simplex virus (HSV) infection has previously been proposed. Thus, the aim of this study was to investigate a potential association between the IL28B host genotype and recurrent HSK. Materials and Methods: Eighty patients older than 18 years of age of both genders with a history of recurrent herpes simplex labialis (HSL) were considered for inclusion. Seventy-five of these patients were found to be seropositive for HSV-1 and were subsequently enrolled in the study. Twenty-four of the enrolled patients also had a history of recurrent HSK associated with severe corneal scarring and visual acuity deterioration. Total DNA was isolated from whole blood samples. A single-nucleotide polymorphism (SNP) rs12979860 near the IL28B gene on chromosome 19 was genotyped. Results: A significant association was observed between recurrent HSK and two SNPs of the IL28B genotype (CCrs12979860 and CTrs12979860, p < 0.01). The variation CCrs12979860 showed a significantly greater association with HSK (16 out of 26 patients) compared with CTrs12979860 (8 out of 34 patients). Conclusion: Seropositive individuals with a history of recurrent HSK are likely to have the CC IL28B genotype. This genotype may be related to incomplete control of the infection and more frequent periodical viral shedding along the first nerve branch of the trigeminal ganglion, which clinically manifests as recurrent herpes keratitis. The clinical manifestation of recurrent HSV-1 infection seems to be influenced by polymorphism of the IL28B genotype. MDPI 2019-09-26 /pmc/articles/PMC6843532/ /pubmed/31561422 http://dx.doi.org/10.3390/medicina55100642 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Borivoje, Savić
Svetlana, Stanojlović
Milan, Hadži-Milić
Nela, Đonović
Olivera, Milošević-Đorđević
Filip, Milisavljević
Milenko, Stojković
Srbislav, Pajić
IL28B Genetic Variations in Patients with Recurrent Herpes Simplex Keratitis
title IL28B Genetic Variations in Patients with Recurrent Herpes Simplex Keratitis
title_full IL28B Genetic Variations in Patients with Recurrent Herpes Simplex Keratitis
title_fullStr IL28B Genetic Variations in Patients with Recurrent Herpes Simplex Keratitis
title_full_unstemmed IL28B Genetic Variations in Patients with Recurrent Herpes Simplex Keratitis
title_short IL28B Genetic Variations in Patients with Recurrent Herpes Simplex Keratitis
title_sort il28b genetic variations in patients with recurrent herpes simplex keratitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6843532/
https://www.ncbi.nlm.nih.gov/pubmed/31561422
http://dx.doi.org/10.3390/medicina55100642
work_keys_str_mv AT borivojesavic il28bgeneticvariationsinpatientswithrecurrentherpessimplexkeratitis
AT svetlanastanojlovic il28bgeneticvariationsinpatientswithrecurrentherpessimplexkeratitis
AT milanhadzimilic il28bgeneticvariationsinpatientswithrecurrentherpessimplexkeratitis
AT nelađonovic il28bgeneticvariationsinpatientswithrecurrentherpessimplexkeratitis
AT oliveramilosevicđorđevic il28bgeneticvariationsinpatientswithrecurrentherpessimplexkeratitis
AT filipmilisavljevic il28bgeneticvariationsinpatientswithrecurrentherpessimplexkeratitis
AT milenkostojkovic il28bgeneticvariationsinpatientswithrecurrentherpessimplexkeratitis
AT srbislavpajic il28bgeneticvariationsinpatientswithrecurrentherpessimplexkeratitis