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An Efficient and Economical Protocol for Isolating, Purifying and PEG-Mediated Transient Gene Expression of Chinese Kale Hypocotyl Protoplasts

In this study, we report the isolation and purification of protoplasts from Chinese kale (Brassica oleracea var. alboglabra) hypocotyls, and their transient gene expression transformation and subcellular localization of BaMYB75 (Bol042409). The upshot is that the vintage protocol included 5-d hypoco...

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Detalles Bibliográficos
Autores principales: Sun, Bo, Yuan, Qiao, Zheng, Hao, Liang, Sha, Jiang, Min, Wang, Mei-Mei, Chen, Qing, Li, Meng-Yao, Zhang, Yong, Luo, Ya, Gong, Rong-Gao, Zhang, Fen, Tang, Hao-Ru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6843555/
https://www.ncbi.nlm.nih.gov/pubmed/31569422
http://dx.doi.org/10.3390/plants8100385

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