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Genetic Characterization and Pathological Analysis of a Novel Bacterial Pathogen, Pseudomonas tructae, in Rainbow Trout (Oncorhynchus mykiss)

Pseudomonas species are one of the most prevalent bacterial species globally distributed in forest soil, river water, and human or animal skin. Some species are pathogens or opportunistic pathogens in hospitalized patients, animals, and plants. Various Pseudomonas species, including Pseudomonas puti...

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Autores principales: Oh, Woo Taek, Kim, Ji Hyung, Jun, Jin Woo, Giri, Sib Sankar, Yun, Saekil, Kim, Hyoun Joong, Kim, Sang Guen, Kim, Sang Wha, Han, Se Jin, Kwon, Jun, Park, Se Chang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6843698/
https://www.ncbi.nlm.nih.gov/pubmed/31658660
http://dx.doi.org/10.3390/microorganisms7100432
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author Oh, Woo Taek
Kim, Ji Hyung
Jun, Jin Woo
Giri, Sib Sankar
Yun, Saekil
Kim, Hyoun Joong
Kim, Sang Guen
Kim, Sang Wha
Han, Se Jin
Kwon, Jun
Park, Se Chang
author_facet Oh, Woo Taek
Kim, Ji Hyung
Jun, Jin Woo
Giri, Sib Sankar
Yun, Saekil
Kim, Hyoun Joong
Kim, Sang Guen
Kim, Sang Wha
Han, Se Jin
Kwon, Jun
Park, Se Chang
author_sort Oh, Woo Taek
collection PubMed
description Pseudomonas species are one of the most prevalent bacterial species globally distributed in forest soil, river water, and human or animal skin. Some species are pathogens or opportunistic pathogens in hospitalized patients, animals, and plants. Various Pseudomonas species, including Pseudomonas putida, P. plecoglossicida, P. aeruginosa, and P. fluorescens, are known fish pathogens; P. fluorescens and P. putida cause severe losses in rainbow trout farming. Therefore, we investigated and isolated the pathogen that is responsible for mortality in a rainbow trout farm in Korea. The isolated bacterium was a strain of P. tructae, which was recently classified in the P. putida group. We performed taxonomical analysis of the bacteria in our previous study. In this study, we investigated the pathogenicity and clinical symptoms of P. tructae and analyzed its genomic characteristics. The pathogenicity of the strain was tested via challenge experiments in healthy rainbow trout and histopathologic analysis of the infected fish. Genome sequence was analyzed to identify the bacterial genes that are involved in antibiotic resistance and virulence. This is the first study reporting P. tructae as an emerging pathogen that is responsible for mortality in rainbow trout fisheries and providing the genome sequence of P. tructae.
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spelling pubmed-68436982019-11-25 Genetic Characterization and Pathological Analysis of a Novel Bacterial Pathogen, Pseudomonas tructae, in Rainbow Trout (Oncorhynchus mykiss) Oh, Woo Taek Kim, Ji Hyung Jun, Jin Woo Giri, Sib Sankar Yun, Saekil Kim, Hyoun Joong Kim, Sang Guen Kim, Sang Wha Han, Se Jin Kwon, Jun Park, Se Chang Microorganisms Article Pseudomonas species are one of the most prevalent bacterial species globally distributed in forest soil, river water, and human or animal skin. Some species are pathogens or opportunistic pathogens in hospitalized patients, animals, and plants. Various Pseudomonas species, including Pseudomonas putida, P. plecoglossicida, P. aeruginosa, and P. fluorescens, are known fish pathogens; P. fluorescens and P. putida cause severe losses in rainbow trout farming. Therefore, we investigated and isolated the pathogen that is responsible for mortality in a rainbow trout farm in Korea. The isolated bacterium was a strain of P. tructae, which was recently classified in the P. putida group. We performed taxonomical analysis of the bacteria in our previous study. In this study, we investigated the pathogenicity and clinical symptoms of P. tructae and analyzed its genomic characteristics. The pathogenicity of the strain was tested via challenge experiments in healthy rainbow trout and histopathologic analysis of the infected fish. Genome sequence was analyzed to identify the bacterial genes that are involved in antibiotic resistance and virulence. This is the first study reporting P. tructae as an emerging pathogen that is responsible for mortality in rainbow trout fisheries and providing the genome sequence of P. tructae. MDPI 2019-10-10 /pmc/articles/PMC6843698/ /pubmed/31658660 http://dx.doi.org/10.3390/microorganisms7100432 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Oh, Woo Taek
Kim, Ji Hyung
Jun, Jin Woo
Giri, Sib Sankar
Yun, Saekil
Kim, Hyoun Joong
Kim, Sang Guen
Kim, Sang Wha
Han, Se Jin
Kwon, Jun
Park, Se Chang
Genetic Characterization and Pathological Analysis of a Novel Bacterial Pathogen, Pseudomonas tructae, in Rainbow Trout (Oncorhynchus mykiss)
title Genetic Characterization and Pathological Analysis of a Novel Bacterial Pathogen, Pseudomonas tructae, in Rainbow Trout (Oncorhynchus mykiss)
title_full Genetic Characterization and Pathological Analysis of a Novel Bacterial Pathogen, Pseudomonas tructae, in Rainbow Trout (Oncorhynchus mykiss)
title_fullStr Genetic Characterization and Pathological Analysis of a Novel Bacterial Pathogen, Pseudomonas tructae, in Rainbow Trout (Oncorhynchus mykiss)
title_full_unstemmed Genetic Characterization and Pathological Analysis of a Novel Bacterial Pathogen, Pseudomonas tructae, in Rainbow Trout (Oncorhynchus mykiss)
title_short Genetic Characterization and Pathological Analysis of a Novel Bacterial Pathogen, Pseudomonas tructae, in Rainbow Trout (Oncorhynchus mykiss)
title_sort genetic characterization and pathological analysis of a novel bacterial pathogen, pseudomonas tructae, in rainbow trout (oncorhynchus mykiss)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6843698/
https://www.ncbi.nlm.nih.gov/pubmed/31658660
http://dx.doi.org/10.3390/microorganisms7100432
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