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Efficient Biolistic Transformation of Immature Citrus Rootstocks Using Phosphomannose-isomerase Selection
This research utilized the E. coli manA gene encoding phosphomannose isomerase (PMI) selection on sucrose/mannose medium to increase transformation efficiencies after biolistic transformation of two immature citrus rootstock cultivars. Plasmid DNA, containing the manA gene and the enhanced green flu...
Autores principales: | Wu, Hao, Acanda, Yosvanis, Canton, Michel, Zale, Janice |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6843792/ https://www.ncbi.nlm.nih.gov/pubmed/31575070 http://dx.doi.org/10.3390/plants8100390 |
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