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Efficient Characterization of Tetraploid Watermelon
Watermelon (Citrullus lanatus (Thunb.) Matsum. &Nakai) is an economic crop, which is widely cultivated around the world. The ploidy study of watermelon has an important role in field breeding and production, therefore, timely and convenient ploidy detection is necessary to accelerate its applica...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6843817/ https://www.ncbi.nlm.nih.gov/pubmed/31623210 http://dx.doi.org/10.3390/plants8100419 |
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author | Zhang, Na Bao, Yaning Xie, Zhouli Huang, Xing Sun, Yuhong Feng, Gang Zeng, Hongxia Ren, Jian Li, Yuhua Xiong, Jianshun Chen, Wei Yan, Chao Tang, Mi |
author_facet | Zhang, Na Bao, Yaning Xie, Zhouli Huang, Xing Sun, Yuhong Feng, Gang Zeng, Hongxia Ren, Jian Li, Yuhua Xiong, Jianshun Chen, Wei Yan, Chao Tang, Mi |
author_sort | Zhang, Na |
collection | PubMed |
description | Watermelon (Citrullus lanatus (Thunb.) Matsum. &Nakai) is an economic crop, which is widely cultivated around the world. The ploidy study of watermelon has an important role in field breeding and production, therefore, timely and convenient ploidy detection is necessary to accelerate its application. Traditionally, the ploidy of watermelon was determined by a series of time-consuming, expensive, and less efficient methods. In this study, we developed a more efficient method to simplify and accelerate the polyploidy identification in watermelons. We first confirmed the ploidy of watermelon by traditional tetraploid morphological features and well-established flow cytometry (FCM). Then we developed a reliable real-time quantitative PCR (qPCR) technique by quantifying the highly conserved 5S rDNA sequence and its copy numbers. This technique requires less sample collection and has comparable accuracy to FCM, it accelerates the analysis process and provides a new method for the identification of polyploidy of watermelon. |
format | Online Article Text |
id | pubmed-6843817 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68438172019-11-25 Efficient Characterization of Tetraploid Watermelon Zhang, Na Bao, Yaning Xie, Zhouli Huang, Xing Sun, Yuhong Feng, Gang Zeng, Hongxia Ren, Jian Li, Yuhua Xiong, Jianshun Chen, Wei Yan, Chao Tang, Mi Plants (Basel) Article Watermelon (Citrullus lanatus (Thunb.) Matsum. &Nakai) is an economic crop, which is widely cultivated around the world. The ploidy study of watermelon has an important role in field breeding and production, therefore, timely and convenient ploidy detection is necessary to accelerate its application. Traditionally, the ploidy of watermelon was determined by a series of time-consuming, expensive, and less efficient methods. In this study, we developed a more efficient method to simplify and accelerate the polyploidy identification in watermelons. We first confirmed the ploidy of watermelon by traditional tetraploid morphological features and well-established flow cytometry (FCM). Then we developed a reliable real-time quantitative PCR (qPCR) technique by quantifying the highly conserved 5S rDNA sequence and its copy numbers. This technique requires less sample collection and has comparable accuracy to FCM, it accelerates the analysis process and provides a new method for the identification of polyploidy of watermelon. MDPI 2019-10-16 /pmc/articles/PMC6843817/ /pubmed/31623210 http://dx.doi.org/10.3390/plants8100419 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Na Bao, Yaning Xie, Zhouli Huang, Xing Sun, Yuhong Feng, Gang Zeng, Hongxia Ren, Jian Li, Yuhua Xiong, Jianshun Chen, Wei Yan, Chao Tang, Mi Efficient Characterization of Tetraploid Watermelon |
title | Efficient Characterization of Tetraploid Watermelon |
title_full | Efficient Characterization of Tetraploid Watermelon |
title_fullStr | Efficient Characterization of Tetraploid Watermelon |
title_full_unstemmed | Efficient Characterization of Tetraploid Watermelon |
title_short | Efficient Characterization of Tetraploid Watermelon |
title_sort | efficient characterization of tetraploid watermelon |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6843817/ https://www.ncbi.nlm.nih.gov/pubmed/31623210 http://dx.doi.org/10.3390/plants8100419 |
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