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Discovery of Serotransferrin Glycoforms: Novel Markers for Diagnosis of Liver Periductal Fibrosis and Prediction of Cholangiocarcinoma

Cholangiocarcinoma (CCA) caused by chronic liver fluke infection is a major public health problem in Northeast Thailand. Identification of CCA risk groups is urgently needed for the control of CCA in this region. Periductal fibrosis (PDF) induced by chronic inflammation of bile ducts is known as a p...

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Autores principales: Jamnongkan, Wassana, Lebrilla, Carlito B., Barboza, Mariana, Techasen, Anchalee, Loilome, Watcharin, Sithithaworn, Paiboon, Khuntikeo, Narong, Pairojkul, Chawalit, Chamadol, Nittaya, Thanan, Raynoo, Yongvanit, Puangrat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6843847/
https://www.ncbi.nlm.nih.gov/pubmed/31569686
http://dx.doi.org/10.3390/biom9100538
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author Jamnongkan, Wassana
Lebrilla, Carlito B.
Barboza, Mariana
Techasen, Anchalee
Loilome, Watcharin
Sithithaworn, Paiboon
Khuntikeo, Narong
Pairojkul, Chawalit
Chamadol, Nittaya
Thanan, Raynoo
Yongvanit, Puangrat
author_facet Jamnongkan, Wassana
Lebrilla, Carlito B.
Barboza, Mariana
Techasen, Anchalee
Loilome, Watcharin
Sithithaworn, Paiboon
Khuntikeo, Narong
Pairojkul, Chawalit
Chamadol, Nittaya
Thanan, Raynoo
Yongvanit, Puangrat
author_sort Jamnongkan, Wassana
collection PubMed
description Cholangiocarcinoma (CCA) caused by chronic liver fluke infection is a major public health problem in Northeast Thailand. Identification of CCA risk groups is urgently needed for the control of CCA in this region. Periductal fibrosis (PDF) induced by chronic inflammation of bile ducts is known as a pre-neoplastic lesion of CCA. We aimed to identify the serum CCA and PDF biomarkers using mass spectrometry (UPLC-ESI-QqQ) with multiple reaction mode (MRM) analysis. Here, serum levels of serotransferrin glycoforms at the glycopeptide level were measured in the sera of CCA (n = 100), PDF (n = 50), and healthy control (n = 100) subjects. The results indicated that serotransferrin peptide levels were generally the same between the control and PDF groups, whereas CCA patients had reduced levels. Moreover, 56 serotransferrin glycoforms were detected, with nine increased in CCA compared to control subjects. Among them, the serum levels of four glycoforms were increased in PDF and CCA patients compared to control subjects. In particular, highly sialylated multi-branched glycans of serotransferrin serum were significantly correlated with poor prognosis and tumor stage in CCA patients. Taken together, these glycoforms could be used as risk biomarkers and prognosis and diagnosis markers of CCA.
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spelling pubmed-68438472019-11-25 Discovery of Serotransferrin Glycoforms: Novel Markers for Diagnosis of Liver Periductal Fibrosis and Prediction of Cholangiocarcinoma Jamnongkan, Wassana Lebrilla, Carlito B. Barboza, Mariana Techasen, Anchalee Loilome, Watcharin Sithithaworn, Paiboon Khuntikeo, Narong Pairojkul, Chawalit Chamadol, Nittaya Thanan, Raynoo Yongvanit, Puangrat Biomolecules Article Cholangiocarcinoma (CCA) caused by chronic liver fluke infection is a major public health problem in Northeast Thailand. Identification of CCA risk groups is urgently needed for the control of CCA in this region. Periductal fibrosis (PDF) induced by chronic inflammation of bile ducts is known as a pre-neoplastic lesion of CCA. We aimed to identify the serum CCA and PDF biomarkers using mass spectrometry (UPLC-ESI-QqQ) with multiple reaction mode (MRM) analysis. Here, serum levels of serotransferrin glycoforms at the glycopeptide level were measured in the sera of CCA (n = 100), PDF (n = 50), and healthy control (n = 100) subjects. The results indicated that serotransferrin peptide levels were generally the same between the control and PDF groups, whereas CCA patients had reduced levels. Moreover, 56 serotransferrin glycoforms were detected, with nine increased in CCA compared to control subjects. Among them, the serum levels of four glycoforms were increased in PDF and CCA patients compared to control subjects. In particular, highly sialylated multi-branched glycans of serotransferrin serum were significantly correlated with poor prognosis and tumor stage in CCA patients. Taken together, these glycoforms could be used as risk biomarkers and prognosis and diagnosis markers of CCA. MDPI 2019-09-27 /pmc/articles/PMC6843847/ /pubmed/31569686 http://dx.doi.org/10.3390/biom9100538 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jamnongkan, Wassana
Lebrilla, Carlito B.
Barboza, Mariana
Techasen, Anchalee
Loilome, Watcharin
Sithithaworn, Paiboon
Khuntikeo, Narong
Pairojkul, Chawalit
Chamadol, Nittaya
Thanan, Raynoo
Yongvanit, Puangrat
Discovery of Serotransferrin Glycoforms: Novel Markers for Diagnosis of Liver Periductal Fibrosis and Prediction of Cholangiocarcinoma
title Discovery of Serotransferrin Glycoforms: Novel Markers for Diagnosis of Liver Periductal Fibrosis and Prediction of Cholangiocarcinoma
title_full Discovery of Serotransferrin Glycoforms: Novel Markers for Diagnosis of Liver Periductal Fibrosis and Prediction of Cholangiocarcinoma
title_fullStr Discovery of Serotransferrin Glycoforms: Novel Markers for Diagnosis of Liver Periductal Fibrosis and Prediction of Cholangiocarcinoma
title_full_unstemmed Discovery of Serotransferrin Glycoforms: Novel Markers for Diagnosis of Liver Periductal Fibrosis and Prediction of Cholangiocarcinoma
title_short Discovery of Serotransferrin Glycoforms: Novel Markers for Diagnosis of Liver Periductal Fibrosis and Prediction of Cholangiocarcinoma
title_sort discovery of serotransferrin glycoforms: novel markers for diagnosis of liver periductal fibrosis and prediction of cholangiocarcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6843847/
https://www.ncbi.nlm.nih.gov/pubmed/31569686
http://dx.doi.org/10.3390/biom9100538
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