Beyond Chelation: EDTA Tightly Binds Taq DNA Polymerase, MutT and dUTPase and Directly Inhibits dNTPase Activity

EDTA is commonly used as an efficient chelator of metal ion enzyme cofactors. It is highly soluble, optically inactive and does not interfere with most chemicals used in standard buffers making EDTA a common choice to generate metal-free conditions for biochemical and biophysical investigations. How...

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Detalles Bibliográficos
Autores principales: Lopata, Anna, Jójárt, Balázs, Surányi, Éva V., Takács, Enikő, Bezúr, László, Leveles, Ibolya, Bendes, Ábris Á., Viskolcz, Béla, Vértessy, Beáta G., Tóth, Judit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6843921/
https://www.ncbi.nlm.nih.gov/pubmed/31627475
http://dx.doi.org/10.3390/biom9100621

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6843921/
https://www.ncbi.nlm.nih.gov/pubmed/31627475
http://dx.doi.org/10.3390/biom9100621

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