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Release kinetics of the model protein FITC-BSA from different polymer-coated bovine bone substitutes

BACKGROUND: Controlled release of proteins bound to conventional bone substitutes is still insufficient. Therefore, this study evaluates in-vitro release kinetics of the model protein FITC-BSA (fluorescein conjugated bovine serum albumine) from insoluble bovine collagenous bone matrices (ICBM) with...

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Autores principales: Lommen, Julian, Schorn, Lara, Landers, Alexis, Holtmann, Henrik, Berr, Karin, Kübler, Norbert R., Sproll, Christoph, Rana, Majeed, Depprich, Rita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6844035/
https://www.ncbi.nlm.nih.gov/pubmed/31711509
http://dx.doi.org/10.1186/s13005-019-0211-y
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author Lommen, Julian
Schorn, Lara
Landers, Alexis
Holtmann, Henrik
Berr, Karin
Kübler, Norbert R.
Sproll, Christoph
Rana, Majeed
Depprich, Rita
author_facet Lommen, Julian
Schorn, Lara
Landers, Alexis
Holtmann, Henrik
Berr, Karin
Kübler, Norbert R.
Sproll, Christoph
Rana, Majeed
Depprich, Rita
author_sort Lommen, Julian
collection PubMed
description BACKGROUND: Controlled release of proteins bound to conventional bone substitutes is still insufficient. Therefore, this study evaluates in-vitro release kinetics of the model protein FITC-BSA (fluorescein conjugated bovine serum albumine) from insoluble bovine collagenous bone matrices (ICBM) with different polymer coatings. Analyzes aim at comparing FITC-BSA release from uncoated versus coated ICBM over time to find bone substitute coatings with consistent release profiles. METHODS: Release kinetics of FITC-BSA from uncoated as well as coated ICBM with five different polymers (RESOMER R 203 H, RG 503 H, RG 504 H, RG 505, L 206 S) were measured over a period of 11 days (d). Measurements were conducted after 6 h (h), 12 h, 24 h, 3 d, 5 d, 7 d, 9 d and 11 d with six samples for each coated ICBM. Two groups were formed (1) with and (2) without medium change at times of measurement. For each group ANOVA with post-hoc Bonferroni testing was used. Scanning electron microscopy assessed morphologic differences between ICBM coating. RESULTS: In group 1 approx. 70% of FITC-BSA release from uncoated ICBM occurred after 6 h compared to approx. 50% in group 2. Only polymers with medium inherent viscosity, i.e. RESOMER RG 503 H, constantly showed significantly more FITC-BSA release throughout 11 d than uncoated ICBM (p = 0.007). The same was found for group 2 (p = 0.005). No significant differences between PLA and PLGA polymers were found. Scanning electron microscopy results indicate a weak adhesion of polymer coatings to ICBM explaining its rather weak retentive effect on overall FITC-BSA release. CONCLUSIONS: Medium molecular size polymers reduce the overall released FITC-BSA from ICBM over time. In clinical practice these polymers may prove ideal for bone substitute materials.
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spelling pubmed-68440352019-11-15 Release kinetics of the model protein FITC-BSA from different polymer-coated bovine bone substitutes Lommen, Julian Schorn, Lara Landers, Alexis Holtmann, Henrik Berr, Karin Kübler, Norbert R. Sproll, Christoph Rana, Majeed Depprich, Rita Head Face Med Research BACKGROUND: Controlled release of proteins bound to conventional bone substitutes is still insufficient. Therefore, this study evaluates in-vitro release kinetics of the model protein FITC-BSA (fluorescein conjugated bovine serum albumine) from insoluble bovine collagenous bone matrices (ICBM) with different polymer coatings. Analyzes aim at comparing FITC-BSA release from uncoated versus coated ICBM over time to find bone substitute coatings with consistent release profiles. METHODS: Release kinetics of FITC-BSA from uncoated as well as coated ICBM with five different polymers (RESOMER R 203 H, RG 503 H, RG 504 H, RG 505, L 206 S) were measured over a period of 11 days (d). Measurements were conducted after 6 h (h), 12 h, 24 h, 3 d, 5 d, 7 d, 9 d and 11 d with six samples for each coated ICBM. Two groups were formed (1) with and (2) without medium change at times of measurement. For each group ANOVA with post-hoc Bonferroni testing was used. Scanning electron microscopy assessed morphologic differences between ICBM coating. RESULTS: In group 1 approx. 70% of FITC-BSA release from uncoated ICBM occurred after 6 h compared to approx. 50% in group 2. Only polymers with medium inherent viscosity, i.e. RESOMER RG 503 H, constantly showed significantly more FITC-BSA release throughout 11 d than uncoated ICBM (p = 0.007). The same was found for group 2 (p = 0.005). No significant differences between PLA and PLGA polymers were found. Scanning electron microscopy results indicate a weak adhesion of polymer coatings to ICBM explaining its rather weak retentive effect on overall FITC-BSA release. CONCLUSIONS: Medium molecular size polymers reduce the overall released FITC-BSA from ICBM over time. In clinical practice these polymers may prove ideal for bone substitute materials. BioMed Central 2019-11-11 /pmc/articles/PMC6844035/ /pubmed/31711509 http://dx.doi.org/10.1186/s13005-019-0211-y Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Lommen, Julian
Schorn, Lara
Landers, Alexis
Holtmann, Henrik
Berr, Karin
Kübler, Norbert R.
Sproll, Christoph
Rana, Majeed
Depprich, Rita
Release kinetics of the model protein FITC-BSA from different polymer-coated bovine bone substitutes
title Release kinetics of the model protein FITC-BSA from different polymer-coated bovine bone substitutes
title_full Release kinetics of the model protein FITC-BSA from different polymer-coated bovine bone substitutes
title_fullStr Release kinetics of the model protein FITC-BSA from different polymer-coated bovine bone substitutes
title_full_unstemmed Release kinetics of the model protein FITC-BSA from different polymer-coated bovine bone substitutes
title_short Release kinetics of the model protein FITC-BSA from different polymer-coated bovine bone substitutes
title_sort release kinetics of the model protein fitc-bsa from different polymer-coated bovine bone substitutes
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6844035/
https://www.ncbi.nlm.nih.gov/pubmed/31711509
http://dx.doi.org/10.1186/s13005-019-0211-y
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