Cargando…

Baicalin Regulates Proliferation, Apoptosis, Migration, and Invasion in Mesothelioma

BACKGROUND: Baicalin, one of the main bioactive components extracted from the traditional Chinese medicine baical Skullcap root, has an anti-tumor activity which had been studied in several cancers. However, its role in human mesothelioma remains unknown. In this study, we investigated the anti-tumo...

Descripción completa

Detalles Bibliográficos
Autores principales: Xu, Wen-Fei, Liu, Feng, Ma, Yi-Cong, Qian, Zhi-Rong, Shi, Long, Mu, Hang, Ding, Feng, Fu, Xue-Qi, Li, Xu-Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6844144/
https://www.ncbi.nlm.nih.gov/pubmed/31670317
http://dx.doi.org/10.12659/MSM.919872
_version_ 1783468379171979264
author Xu, Wen-Fei
Liu, Feng
Ma, Yi-Cong
Qian, Zhi-Rong
Shi, Long
Mu, Hang
Ding, Feng
Fu, Xue-Qi
Li, Xu-Hui
author_facet Xu, Wen-Fei
Liu, Feng
Ma, Yi-Cong
Qian, Zhi-Rong
Shi, Long
Mu, Hang
Ding, Feng
Fu, Xue-Qi
Li, Xu-Hui
author_sort Xu, Wen-Fei
collection PubMed
description BACKGROUND: Baicalin, one of the main bioactive components extracted from the traditional Chinese medicine baical Skullcap root, has an anti-tumor activity which had been studied in several cancers. However, its role in human mesothelioma remains unknown. In this study, we investigated the anti-tumor mechanisms of baicalin in the mesothelioma cell line MESO924. MATERIAL/METHODS: Effects of baicalin on mesothelioma were assessed by measuring cell viability, apoptosis, migration, invasion, inactivation of signaling intermediates, and cell-cycle alterations. RESULTS: Baicalin inhibited the proliferation, migration, and invasion of human mesothelioma cells and increased their apoptosis, all in a dose-dependent manner. Specifically, baicalin decreased the expression of p-EGFR, p-AKT, p-MAPK, p-S6, Bcl-2, and VEGF and increased the expression of Bax in mesothelioma cells. The suppressed mesothelioma cellular proliferation is due to the arrest of the S cell cycle by baicalin. Inhibition of the PI3K/AKT/mTOR signaling pathway by a PI3K/AKT/mTOR inhibitor augmented the anti-proliferation effects induced by baicalin. In addition, baicalin increased the sensitivity of MESO924 to the chemotherapeutic drugs doxorubicin, cisplatin, and pemetrexed. CONCLUSIONS: These results highlight the roles of baicalin in inhibiting cell growth, migration, and invasion of mesothelioma cells while increasing apoptosis and sensitizing cells to chemotherapeutic agents through the PI3K/AKT/mTOR signaling pathway, which indicates that baicalin could be a useful drug for mesothelioma therapy.
format Online
Article
Text
id pubmed-6844144
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher International Scientific Literature, Inc.
record_format MEDLINE/PubMed
spelling pubmed-68441442019-11-18 Baicalin Regulates Proliferation, Apoptosis, Migration, and Invasion in Mesothelioma Xu, Wen-Fei Liu, Feng Ma, Yi-Cong Qian, Zhi-Rong Shi, Long Mu, Hang Ding, Feng Fu, Xue-Qi Li, Xu-Hui Med Sci Monit Lab/In Vitro Research BACKGROUND: Baicalin, one of the main bioactive components extracted from the traditional Chinese medicine baical Skullcap root, has an anti-tumor activity which had been studied in several cancers. However, its role in human mesothelioma remains unknown. In this study, we investigated the anti-tumor mechanisms of baicalin in the mesothelioma cell line MESO924. MATERIAL/METHODS: Effects of baicalin on mesothelioma were assessed by measuring cell viability, apoptosis, migration, invasion, inactivation of signaling intermediates, and cell-cycle alterations. RESULTS: Baicalin inhibited the proliferation, migration, and invasion of human mesothelioma cells and increased their apoptosis, all in a dose-dependent manner. Specifically, baicalin decreased the expression of p-EGFR, p-AKT, p-MAPK, p-S6, Bcl-2, and VEGF and increased the expression of Bax in mesothelioma cells. The suppressed mesothelioma cellular proliferation is due to the arrest of the S cell cycle by baicalin. Inhibition of the PI3K/AKT/mTOR signaling pathway by a PI3K/AKT/mTOR inhibitor augmented the anti-proliferation effects induced by baicalin. In addition, baicalin increased the sensitivity of MESO924 to the chemotherapeutic drugs doxorubicin, cisplatin, and pemetrexed. CONCLUSIONS: These results highlight the roles of baicalin in inhibiting cell growth, migration, and invasion of mesothelioma cells while increasing apoptosis and sensitizing cells to chemotherapeutic agents through the PI3K/AKT/mTOR signaling pathway, which indicates that baicalin could be a useful drug for mesothelioma therapy. International Scientific Literature, Inc. 2019-10-31 /pmc/articles/PMC6844144/ /pubmed/31670317 http://dx.doi.org/10.12659/MSM.919872 Text en © Med Sci Monit, 2019 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) )
spellingShingle Lab/In Vitro Research
Xu, Wen-Fei
Liu, Feng
Ma, Yi-Cong
Qian, Zhi-Rong
Shi, Long
Mu, Hang
Ding, Feng
Fu, Xue-Qi
Li, Xu-Hui
Baicalin Regulates Proliferation, Apoptosis, Migration, and Invasion in Mesothelioma
title Baicalin Regulates Proliferation, Apoptosis, Migration, and Invasion in Mesothelioma
title_full Baicalin Regulates Proliferation, Apoptosis, Migration, and Invasion in Mesothelioma
title_fullStr Baicalin Regulates Proliferation, Apoptosis, Migration, and Invasion in Mesothelioma
title_full_unstemmed Baicalin Regulates Proliferation, Apoptosis, Migration, and Invasion in Mesothelioma
title_short Baicalin Regulates Proliferation, Apoptosis, Migration, and Invasion in Mesothelioma
title_sort baicalin regulates proliferation, apoptosis, migration, and invasion in mesothelioma
topic Lab/In Vitro Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6844144/
https://www.ncbi.nlm.nih.gov/pubmed/31670317
http://dx.doi.org/10.12659/MSM.919872
work_keys_str_mv AT xuwenfei baicalinregulatesproliferationapoptosismigrationandinvasioninmesothelioma
AT liufeng baicalinregulatesproliferationapoptosismigrationandinvasioninmesothelioma
AT mayicong baicalinregulatesproliferationapoptosismigrationandinvasioninmesothelioma
AT qianzhirong baicalinregulatesproliferationapoptosismigrationandinvasioninmesothelioma
AT shilong baicalinregulatesproliferationapoptosismigrationandinvasioninmesothelioma
AT muhang baicalinregulatesproliferationapoptosismigrationandinvasioninmesothelioma
AT dingfeng baicalinregulatesproliferationapoptosismigrationandinvasioninmesothelioma
AT fuxueqi baicalinregulatesproliferationapoptosismigrationandinvasioninmesothelioma
AT lixuhui baicalinregulatesproliferationapoptosismigrationandinvasioninmesothelioma