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Boronate affinity-based photoactivatable magnetic nanoparticles for the oriented and irreversible conjugation of Fc-fused lectins and antibodies
The utilization of immuno-magnetic nanoparticles (MNPs) for the selective capture, enrichment, and separation of specific glycoproteins from complicated biological samples is appealing for the discovery of disease biomarkers. Herein, MNPs were designed and anchored with abundant boronic acid (BA) an...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royal Society of Chemistry
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6844280/ https://www.ncbi.nlm.nih.gov/pubmed/31803435 http://dx.doi.org/10.1039/c9sc01613a |
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author | Fan, Chen-Yo Hou, Yi-Ren Adak, Avijit K. Waniwan, Juanilita T. dela Rosa, Mira Anne C. Low, Penk Yeir Angata, Takashi Hwang, Kuo-Chu Chen, Yu-Ju Lin, Chun-Cheng |
author_facet | Fan, Chen-Yo Hou, Yi-Ren Adak, Avijit K. Waniwan, Juanilita T. dela Rosa, Mira Anne C. Low, Penk Yeir Angata, Takashi Hwang, Kuo-Chu Chen, Yu-Ju Lin, Chun-Cheng |
author_sort | Fan, Chen-Yo |
collection | PubMed |
description | The utilization of immuno-magnetic nanoparticles (MNPs) for the selective capture, enrichment, and separation of specific glycoproteins from complicated biological samples is appealing for the discovery of disease biomarkers. Herein, MNPs were designed and anchored with abundant boronic acid (BA) and photoreactive alkyl diazirine (Diaz) functional groups to obtain permanently tethered Fc-fused Siglec-2 and antiserum amyloid A (SAA) mAb with the assistance of reversible boronate affinity and UV light activation in an orientation-controlled manner. The Siglec-2–Fc-functionalized MNPs showed excellent stability in fetal bovine serum (FBS) and excellent efficiency in the extraction of cell membrane glycoproteins. The anti-SAA mAb-functionalized MNPs maintained active Ab orientation and preserved antigen recognition capability in biological samples. Thus, the BA–Diaz-based strategy holds promise for the immobilization of glycoproteins, such as antibodies, with the original protein binding activity maintained, which can provide better enrichment for the sensitive detection of target proteins. |
format | Online Article Text |
id | pubmed-6844280 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-68442802019-12-04 Boronate affinity-based photoactivatable magnetic nanoparticles for the oriented and irreversible conjugation of Fc-fused lectins and antibodies Fan, Chen-Yo Hou, Yi-Ren Adak, Avijit K. Waniwan, Juanilita T. dela Rosa, Mira Anne C. Low, Penk Yeir Angata, Takashi Hwang, Kuo-Chu Chen, Yu-Ju Lin, Chun-Cheng Chem Sci Chemistry The utilization of immuno-magnetic nanoparticles (MNPs) for the selective capture, enrichment, and separation of specific glycoproteins from complicated biological samples is appealing for the discovery of disease biomarkers. Herein, MNPs were designed and anchored with abundant boronic acid (BA) and photoreactive alkyl diazirine (Diaz) functional groups to obtain permanently tethered Fc-fused Siglec-2 and antiserum amyloid A (SAA) mAb with the assistance of reversible boronate affinity and UV light activation in an orientation-controlled manner. The Siglec-2–Fc-functionalized MNPs showed excellent stability in fetal bovine serum (FBS) and excellent efficiency in the extraction of cell membrane glycoproteins. The anti-SAA mAb-functionalized MNPs maintained active Ab orientation and preserved antigen recognition capability in biological samples. Thus, the BA–Diaz-based strategy holds promise for the immobilization of glycoproteins, such as antibodies, with the original protein binding activity maintained, which can provide better enrichment for the sensitive detection of target proteins. Royal Society of Chemistry 2019-08-05 /pmc/articles/PMC6844280/ /pubmed/31803435 http://dx.doi.org/10.1039/c9sc01613a Text en This journal is © The Royal Society of Chemistry 2019 http://creativecommons.org/licenses/by/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (CC BY 3.0) |
spellingShingle | Chemistry Fan, Chen-Yo Hou, Yi-Ren Adak, Avijit K. Waniwan, Juanilita T. dela Rosa, Mira Anne C. Low, Penk Yeir Angata, Takashi Hwang, Kuo-Chu Chen, Yu-Ju Lin, Chun-Cheng Boronate affinity-based photoactivatable magnetic nanoparticles for the oriented and irreversible conjugation of Fc-fused lectins and antibodies |
title | Boronate affinity-based photoactivatable magnetic nanoparticles for the oriented and irreversible conjugation of Fc-fused lectins and antibodies
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title_full | Boronate affinity-based photoactivatable magnetic nanoparticles for the oriented and irreversible conjugation of Fc-fused lectins and antibodies
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title_fullStr | Boronate affinity-based photoactivatable magnetic nanoparticles for the oriented and irreversible conjugation of Fc-fused lectins and antibodies
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title_full_unstemmed | Boronate affinity-based photoactivatable magnetic nanoparticles for the oriented and irreversible conjugation of Fc-fused lectins and antibodies
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title_short | Boronate affinity-based photoactivatable magnetic nanoparticles for the oriented and irreversible conjugation of Fc-fused lectins and antibodies
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title_sort | boronate affinity-based photoactivatable magnetic nanoparticles for the oriented and irreversible conjugation of fc-fused lectins and antibodies |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6844280/ https://www.ncbi.nlm.nih.gov/pubmed/31803435 http://dx.doi.org/10.1039/c9sc01613a |
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