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Co-expression of cellulase and xylanase genes in Sacchromyces cerevisiae toward enhanced bioethanol production from corn stover
Lignocellulose is considered as a good resource for producing renewable energy. Previous in vitro studies have shown the synergistic action between cellulase and xylanase during lignocellulose biohydrolysis. In order to achieve the same effect in S. cerevisiae to enhance the practical biotransformat...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6844370/ https://www.ncbi.nlm.nih.gov/pubmed/31661645 http://dx.doi.org/10.1080/21655979.2019.1682213 |
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author | Xiao, Wenjing Li, Huanan Xia, Wucheng Yang, Yuxian Hu, Pan Zhou, Shanna Hu, Yanmei Liu, Xiaopeng Dai, Yujun Jiang, Zhengbing |
author_facet | Xiao, Wenjing Li, Huanan Xia, Wucheng Yang, Yuxian Hu, Pan Zhou, Shanna Hu, Yanmei Liu, Xiaopeng Dai, Yujun Jiang, Zhengbing |
author_sort | Xiao, Wenjing |
collection | PubMed |
description | Lignocellulose is considered as a good resource for producing renewable energy. Previous in vitro studies have shown the synergistic action between cellulase and xylanase during lignocellulose biohydrolysis. In order to achieve the same effect in S. cerevisiae to enhance the practical biotransformation, two recombinant Saccharomyces cerevisiae strains (INVSc1-CBH-CA and INVSc1-CBH-TS) with co-expressed cellulase and xylanase were constructed. The cellulase and xylanase activities in INVSc1-CBH-CA and INVSc1-CBH-TS were 716.43 U/mL and 205.13 U/mL, 931.27 U/mL and 413.70 U/mL, respectively. The recombinant S. cerevisiae can use the partly delignified corn stover (PDCS) more efficiently and more ethanol producted than S. cerevisiae only expressing cellulase. Fermentation with INVSc1-CBH-CA and INVSc1-CBH-TS using PDCS ethanol yields increased by 1.7 and 2.1 folds higher than INVSc1-CBH, 2.8 and 3.4 folds higher than the wild type S. cerevisiae. The strategy of co-expression cellulase and xylanase in saccharomyces cerevisiae is effective and can be a foundation to research the mechanism of synergy effect of cellulose and xylanase. |
format | Online Article Text |
id | pubmed-6844370 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-68443702020-10-29 Co-expression of cellulase and xylanase genes in Sacchromyces cerevisiae toward enhanced bioethanol production from corn stover Xiao, Wenjing Li, Huanan Xia, Wucheng Yang, Yuxian Hu, Pan Zhou, Shanna Hu, Yanmei Liu, Xiaopeng Dai, Yujun Jiang, Zhengbing Bioengineered Research Paper Lignocellulose is considered as a good resource for producing renewable energy. Previous in vitro studies have shown the synergistic action between cellulase and xylanase during lignocellulose biohydrolysis. In order to achieve the same effect in S. cerevisiae to enhance the practical biotransformation, two recombinant Saccharomyces cerevisiae strains (INVSc1-CBH-CA and INVSc1-CBH-TS) with co-expressed cellulase and xylanase were constructed. The cellulase and xylanase activities in INVSc1-CBH-CA and INVSc1-CBH-TS were 716.43 U/mL and 205.13 U/mL, 931.27 U/mL and 413.70 U/mL, respectively. The recombinant S. cerevisiae can use the partly delignified corn stover (PDCS) more efficiently and more ethanol producted than S. cerevisiae only expressing cellulase. Fermentation with INVSc1-CBH-CA and INVSc1-CBH-TS using PDCS ethanol yields increased by 1.7 and 2.1 folds higher than INVSc1-CBH, 2.8 and 3.4 folds higher than the wild type S. cerevisiae. The strategy of co-expression cellulase and xylanase in saccharomyces cerevisiae is effective and can be a foundation to research the mechanism of synergy effect of cellulose and xylanase. Taylor & Francis 2019-10-29 /pmc/articles/PMC6844370/ /pubmed/31661645 http://dx.doi.org/10.1080/21655979.2019.1682213 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Xiao, Wenjing Li, Huanan Xia, Wucheng Yang, Yuxian Hu, Pan Zhou, Shanna Hu, Yanmei Liu, Xiaopeng Dai, Yujun Jiang, Zhengbing Co-expression of cellulase and xylanase genes in Sacchromyces cerevisiae toward enhanced bioethanol production from corn stover |
title | Co-expression of cellulase and xylanase genes in Sacchromyces cerevisiae toward enhanced bioethanol production from corn stover |
title_full | Co-expression of cellulase and xylanase genes in Sacchromyces cerevisiae toward enhanced bioethanol production from corn stover |
title_fullStr | Co-expression of cellulase and xylanase genes in Sacchromyces cerevisiae toward enhanced bioethanol production from corn stover |
title_full_unstemmed | Co-expression of cellulase and xylanase genes in Sacchromyces cerevisiae toward enhanced bioethanol production from corn stover |
title_short | Co-expression of cellulase and xylanase genes in Sacchromyces cerevisiae toward enhanced bioethanol production from corn stover |
title_sort | co-expression of cellulase and xylanase genes in sacchromyces cerevisiae toward enhanced bioethanol production from corn stover |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6844370/ https://www.ncbi.nlm.nih.gov/pubmed/31661645 http://dx.doi.org/10.1080/21655979.2019.1682213 |
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