Effect of F(1) and F(2) generations on genetic variability and working steps of doubled haploid production in maize

For doubled haploid (DH) production in maize, F(1) generation has been the most frequently used for haploid induction due to facility in the process. However, using F(2) generation would be a good alternative to increase genetic variability owing to the additional recombination in meiosis. Our goals...

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Autores principales: Couto, Evellyn Giselly de Oliveira, Cury, Mayara Neves, Bandeira e Souza, Massaine, Granato, Ítalo Stefanine Correia, Vidotti, Miriam Suzane, Domingos Garbuglio, Deoclécio, Crossa, José, Burgueño, Juan, Fritsche-Neto, Roberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6844450/
https://www.ncbi.nlm.nih.gov/pubmed/31710611
http://dx.doi.org/10.1371/journal.pone.0224631
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author Couto, Evellyn Giselly de Oliveira
Cury, Mayara Neves
Bandeira e Souza, Massaine
Granato, Ítalo Stefanine Correia
Vidotti, Miriam Suzane
Domingos Garbuglio, Deoclécio
Crossa, José
Burgueño, Juan
Fritsche-Neto, Roberto
author_facet Couto, Evellyn Giselly de Oliveira
Cury, Mayara Neves
Bandeira e Souza, Massaine
Granato, Ítalo Stefanine Correia
Vidotti, Miriam Suzane
Domingos Garbuglio, Deoclécio
Crossa, José
Burgueño, Juan
Fritsche-Neto, Roberto
author_sort Couto, Evellyn Giselly de Oliveira
collection PubMed
description For doubled haploid (DH) production in maize, F(1) generation has been the most frequently used for haploid induction due to facility in the process. However, using F(2) generation would be a good alternative to increase genetic variability owing to the additional recombination in meiosis. Our goals were to compare the effect of F(1) and F(2) generations on DH production in tropical germplasm, evaluating the R1-navajo expression in seeds, the working steps of the methodology, and the genetic variability of the DH lines obtained. Sources germplasm in F(1) and F(2) generations were crossed with the tropicalized haploid inducer LI-ESALQ. After harvest, for both induction crosses were calculated the haploid induction rate (HIR), diploid seed rate (DSR), and inhibition seed rate (ISR) using the total number of seeds obtained. In order to study the effectiveness of the DH working steps in each generation, the percentage per se and the relative percentage were verified. In addition, SNP markers were obtained for genetic variability studies. Results showed that the values for HIR, ISR, and DSR were 1.23%, 23.48%, and 75.21% for F(1) and 1.78%, 15.82%, and 82.38% for F(2), respectively. The effectiveness of the DH working step showed the same percentage per se value (0.4%) for F(1) and F(2), while the relative percentage was 27.2% for F(1) and 22.4% for F(2). Estimates of population parameters in DH lines from F(1) were higher than F(2). Furthermore, population structure and kinship analyses showed that one additional generation was not sufficient to create new genotype subgroups. Additionally, the relative efficiency of the response to selection in the F(1) was 31.88% higher than F(2) due to the number of cycles that are used to obtain the DH. Our results showed that in tropical maize, the use of F(1) generation is recommended due to a superior balance between time and genetic variability.
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spelling pubmed-68444502019-11-15 Effect of F(1) and F(2) generations on genetic variability and working steps of doubled haploid production in maize Couto, Evellyn Giselly de Oliveira Cury, Mayara Neves Bandeira e Souza, Massaine Granato, Ítalo Stefanine Correia Vidotti, Miriam Suzane Domingos Garbuglio, Deoclécio Crossa, José Burgueño, Juan Fritsche-Neto, Roberto PLoS One Research Article For doubled haploid (DH) production in maize, F(1) generation has been the most frequently used for haploid induction due to facility in the process. However, using F(2) generation would be a good alternative to increase genetic variability owing to the additional recombination in meiosis. Our goals were to compare the effect of F(1) and F(2) generations on DH production in tropical germplasm, evaluating the R1-navajo expression in seeds, the working steps of the methodology, and the genetic variability of the DH lines obtained. Sources germplasm in F(1) and F(2) generations were crossed with the tropicalized haploid inducer LI-ESALQ. After harvest, for both induction crosses were calculated the haploid induction rate (HIR), diploid seed rate (DSR), and inhibition seed rate (ISR) using the total number of seeds obtained. In order to study the effectiveness of the DH working steps in each generation, the percentage per se and the relative percentage were verified. In addition, SNP markers were obtained for genetic variability studies. Results showed that the values for HIR, ISR, and DSR were 1.23%, 23.48%, and 75.21% for F(1) and 1.78%, 15.82%, and 82.38% for F(2), respectively. The effectiveness of the DH working step showed the same percentage per se value (0.4%) for F(1) and F(2), while the relative percentage was 27.2% for F(1) and 22.4% for F(2). Estimates of population parameters in DH lines from F(1) were higher than F(2). Furthermore, population structure and kinship analyses showed that one additional generation was not sufficient to create new genotype subgroups. Additionally, the relative efficiency of the response to selection in the F(1) was 31.88% higher than F(2) due to the number of cycles that are used to obtain the DH. Our results showed that in tropical maize, the use of F(1) generation is recommended due to a superior balance between time and genetic variability. Public Library of Science 2019-11-11 /pmc/articles/PMC6844450/ /pubmed/31710611 http://dx.doi.org/10.1371/journal.pone.0224631 Text en © 2019 Couto et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Couto, Evellyn Giselly de Oliveira
Cury, Mayara Neves
Bandeira e Souza, Massaine
Granato, Ítalo Stefanine Correia
Vidotti, Miriam Suzane
Domingos Garbuglio, Deoclécio
Crossa, José
Burgueño, Juan
Fritsche-Neto, Roberto
Effect of F(1) and F(2) generations on genetic variability and working steps of doubled haploid production in maize
title Effect of F(1) and F(2) generations on genetic variability and working steps of doubled haploid production in maize
title_full Effect of F(1) and F(2) generations on genetic variability and working steps of doubled haploid production in maize
title_fullStr Effect of F(1) and F(2) generations on genetic variability and working steps of doubled haploid production in maize
title_full_unstemmed Effect of F(1) and F(2) generations on genetic variability and working steps of doubled haploid production in maize
title_short Effect of F(1) and F(2) generations on genetic variability and working steps of doubled haploid production in maize
title_sort effect of f(1) and f(2) generations on genetic variability and working steps of doubled haploid production in maize
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6844450/
https://www.ncbi.nlm.nih.gov/pubmed/31710611
http://dx.doi.org/10.1371/journal.pone.0224631
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