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Modular organization of cerebellar climbing fiber inputs during goal-directed behavior
The cerebellum has a parasagittal modular architecture characterized by precisely organized climbing fiber (CF) projections that are congruent with alternating aldolase C/zebrin II expression. However, the behavioral relevance of CF inputs into individual modules remains poorly understood. Here, we...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6844646/ https://www.ncbi.nlm.nih.gov/pubmed/31596238 http://dx.doi.org/10.7554/eLife.47021 |
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author | Tsutsumi, Shinichiro Hidaka, Naoki Isomura, Yoshikazu Matsuzaki, Masanori Sakimura, Kenji Kano, Masanobu Kitamura, Kazuo |
author_facet | Tsutsumi, Shinichiro Hidaka, Naoki Isomura, Yoshikazu Matsuzaki, Masanori Sakimura, Kenji Kano, Masanobu Kitamura, Kazuo |
author_sort | Tsutsumi, Shinichiro |
collection | PubMed |
description | The cerebellum has a parasagittal modular architecture characterized by precisely organized climbing fiber (CF) projections that are congruent with alternating aldolase C/zebrin II expression. However, the behavioral relevance of CF inputs into individual modules remains poorly understood. Here, we used two-photon calcium imaging in the cerebellar hemisphere Crus II in mice performing an auditory go/no-go task to investigate the functional differences in CF inputs to modules. CF signals in medial modules show anticipatory decreases, early increases, secondary increases, and reward-related increases or decreases, which represent quick motor initiation, go cues, fast motor behavior, and positive reward outcomes. CF signals in lateral modules show early increases and reward-related decreases, which represent no-go and/or go cues and positive reward outcomes. The boundaries of CF functions broadly correspond to those of aldolase C patterning. These results indicate that spatially segregated CF inputs in different modules play distinct roles in the execution of goal-directed behavior. |
format | Online Article Text |
id | pubmed-6844646 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-68446462019-11-13 Modular organization of cerebellar climbing fiber inputs during goal-directed behavior Tsutsumi, Shinichiro Hidaka, Naoki Isomura, Yoshikazu Matsuzaki, Masanori Sakimura, Kenji Kano, Masanobu Kitamura, Kazuo eLife Neuroscience The cerebellum has a parasagittal modular architecture characterized by precisely organized climbing fiber (CF) projections that are congruent with alternating aldolase C/zebrin II expression. However, the behavioral relevance of CF inputs into individual modules remains poorly understood. Here, we used two-photon calcium imaging in the cerebellar hemisphere Crus II in mice performing an auditory go/no-go task to investigate the functional differences in CF inputs to modules. CF signals in medial modules show anticipatory decreases, early increases, secondary increases, and reward-related increases or decreases, which represent quick motor initiation, go cues, fast motor behavior, and positive reward outcomes. CF signals in lateral modules show early increases and reward-related decreases, which represent no-go and/or go cues and positive reward outcomes. The boundaries of CF functions broadly correspond to those of aldolase C patterning. These results indicate that spatially segregated CF inputs in different modules play distinct roles in the execution of goal-directed behavior. eLife Sciences Publications, Ltd 2019-10-09 /pmc/articles/PMC6844646/ /pubmed/31596238 http://dx.doi.org/10.7554/eLife.47021 Text en © 2019, Tsutsumi et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Tsutsumi, Shinichiro Hidaka, Naoki Isomura, Yoshikazu Matsuzaki, Masanori Sakimura, Kenji Kano, Masanobu Kitamura, Kazuo Modular organization of cerebellar climbing fiber inputs during goal-directed behavior |
title | Modular organization of cerebellar climbing fiber inputs during goal-directed behavior |
title_full | Modular organization of cerebellar climbing fiber inputs during goal-directed behavior |
title_fullStr | Modular organization of cerebellar climbing fiber inputs during goal-directed behavior |
title_full_unstemmed | Modular organization of cerebellar climbing fiber inputs during goal-directed behavior |
title_short | Modular organization of cerebellar climbing fiber inputs during goal-directed behavior |
title_sort | modular organization of cerebellar climbing fiber inputs during goal-directed behavior |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6844646/ https://www.ncbi.nlm.nih.gov/pubmed/31596238 http://dx.doi.org/10.7554/eLife.47021 |
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