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Characterisation of blood microbiota in patients with liver cirrhosis
Bacterial infections arising in patients with liver cirrhosis are associated with life-threatening complications such as hepatic encephalopathy and spontaneous bacterial peritonitis in relation to bacterial translocation. To investigate the state of bacterial translocation, we surveyed the periphera...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Lippincott Williams And Wilkins
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6844652/ https://www.ncbi.nlm.nih.gov/pubmed/31441799 http://dx.doi.org/10.1097/MEG.0000000000001494 |
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author | Kajihara, Mikio Koido, Shigeo Kanai, Tomoya Ito, Zensho Matsumoto, Yoshihiro Takakura, Kazuki Saruta, Masayuki Kato, Kumiko Odamaki, Toshitaka Xiao, Jin-zhong Sato, Nobuhiro Ohkusa, Toshifumi |
author_facet | Kajihara, Mikio Koido, Shigeo Kanai, Tomoya Ito, Zensho Matsumoto, Yoshihiro Takakura, Kazuki Saruta, Masayuki Kato, Kumiko Odamaki, Toshitaka Xiao, Jin-zhong Sato, Nobuhiro Ohkusa, Toshifumi |
author_sort | Kajihara, Mikio |
collection | PubMed |
description | Bacterial infections arising in patients with liver cirrhosis are associated with life-threatening complications such as hepatic encephalopathy and spontaneous bacterial peritonitis in relation to bacterial translocation. To investigate the state of bacterial translocation, we surveyed the peripheral blood microbiota by 16S rRNA gene sequencing and analysed the blood microbial profiles. METHODS: Sixty-six patients with liver cirrhosis were enrolled in this study, whereas 14 healthy individuals were also analysed as controls. Total RNA was purified from the peripheral blood, and an approximately 430 base pair genomic locus which included the V3-V4 region of the 16s rRNA gene was amplified and assessed using bacterial pyrosequencing. RESULTS: At the genus level, a total of 183 operational taxonomic units were identified in cirrhotic patients, whereas 123 units in controls. Intergroup differences in gut microbiota were analysed by the linear discriminant analysis effect size, which showed that the abundance of Enterobacteriaceae was significantly higher in cirrhotics. On the contrary, the abundance of Akkermansia, Rikenellaceae and Erysipelotrichales were significantly lower in cirrhotics (relative abundance of Akkermansia in cirrhotics and controls: 0% and 0.2%, respectively; Rikenellaceae: 0.1% and 0.92%; Erysipelotrichales: 0.58% and 1.21%). CONCLUSION: The present study has demonstrated that various circulating bacteria are present in cirrhotic patients, and the diversity of such bacteria is consistent with the presence of dysbiosis in cirrhotics. Although the clinical significance of the findings remains to be clarified, the findings may potentially facilitate diagnostic and therapeutic attempts to comprehend and furthermore to manipulate bacterial infections in cirrhotic patients. |
format | Online Article Text |
id | pubmed-6844652 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Lippincott Williams And Wilkins |
record_format | MEDLINE/PubMed |
spelling | pubmed-68446522019-11-26 Characterisation of blood microbiota in patients with liver cirrhosis Kajihara, Mikio Koido, Shigeo Kanai, Tomoya Ito, Zensho Matsumoto, Yoshihiro Takakura, Kazuki Saruta, Masayuki Kato, Kumiko Odamaki, Toshitaka Xiao, Jin-zhong Sato, Nobuhiro Ohkusa, Toshifumi Eur J Gastroenterol Hepatol Original Articles: Hepatology Bacterial infections arising in patients with liver cirrhosis are associated with life-threatening complications such as hepatic encephalopathy and spontaneous bacterial peritonitis in relation to bacterial translocation. To investigate the state of bacterial translocation, we surveyed the peripheral blood microbiota by 16S rRNA gene sequencing and analysed the blood microbial profiles. METHODS: Sixty-six patients with liver cirrhosis were enrolled in this study, whereas 14 healthy individuals were also analysed as controls. Total RNA was purified from the peripheral blood, and an approximately 430 base pair genomic locus which included the V3-V4 region of the 16s rRNA gene was amplified and assessed using bacterial pyrosequencing. RESULTS: At the genus level, a total of 183 operational taxonomic units were identified in cirrhotic patients, whereas 123 units in controls. Intergroup differences in gut microbiota were analysed by the linear discriminant analysis effect size, which showed that the abundance of Enterobacteriaceae was significantly higher in cirrhotics. On the contrary, the abundance of Akkermansia, Rikenellaceae and Erysipelotrichales were significantly lower in cirrhotics (relative abundance of Akkermansia in cirrhotics and controls: 0% and 0.2%, respectively; Rikenellaceae: 0.1% and 0.92%; Erysipelotrichales: 0.58% and 1.21%). CONCLUSION: The present study has demonstrated that various circulating bacteria are present in cirrhotic patients, and the diversity of such bacteria is consistent with the presence of dysbiosis in cirrhotics. Although the clinical significance of the findings remains to be clarified, the findings may potentially facilitate diagnostic and therapeutic attempts to comprehend and furthermore to manipulate bacterial infections in cirrhotic patients. Lippincott Williams And Wilkins 2019-12 2019-08-06 /pmc/articles/PMC6844652/ /pubmed/31441799 http://dx.doi.org/10.1097/MEG.0000000000001494 Text en Copyright © 2019 The Author(s). Published by Wolters Kluwer Health, Inc. This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (https://creativecommons.org/licenses/by/4.0/) (CCBY-NCND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. |
spellingShingle | Original Articles: Hepatology Kajihara, Mikio Koido, Shigeo Kanai, Tomoya Ito, Zensho Matsumoto, Yoshihiro Takakura, Kazuki Saruta, Masayuki Kato, Kumiko Odamaki, Toshitaka Xiao, Jin-zhong Sato, Nobuhiro Ohkusa, Toshifumi Characterisation of blood microbiota in patients with liver cirrhosis |
title | Characterisation of blood microbiota in patients with liver cirrhosis |
title_full | Characterisation of blood microbiota in patients with liver cirrhosis |
title_fullStr | Characterisation of blood microbiota in patients with liver cirrhosis |
title_full_unstemmed | Characterisation of blood microbiota in patients with liver cirrhosis |
title_short | Characterisation of blood microbiota in patients with liver cirrhosis |
title_sort | characterisation of blood microbiota in patients with liver cirrhosis |
topic | Original Articles: Hepatology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6844652/ https://www.ncbi.nlm.nih.gov/pubmed/31441799 http://dx.doi.org/10.1097/MEG.0000000000001494 |
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