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RNA binding candidates for human ADAR3 from substrates of a gain of function mutant expressed in neuronal cells

Human ADAR3 is a catalytically inactive member of the Adenosine Deaminase Acting on RNA (ADAR) protein family, whose active members catalyze A-to-I RNA editing in metazoans. Until now, the reasons for the catalytic incapability of ADAR3 has not been defined and its biological function rarely explore...

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Autores principales: Wang, Yuru, Chung, Dong hee, Monteleone, Leanna R, Li, Jie, Chiang, Yao, Toney, Michael D, Beal, Peter A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6846710/
https://www.ncbi.nlm.nih.gov/pubmed/31552420
http://dx.doi.org/10.1093/nar/gkz815
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author Wang, Yuru
Chung, Dong hee
Monteleone, Leanna R
Li, Jie
Chiang, Yao
Toney, Michael D
Beal, Peter A
author_facet Wang, Yuru
Chung, Dong hee
Monteleone, Leanna R
Li, Jie
Chiang, Yao
Toney, Michael D
Beal, Peter A
author_sort Wang, Yuru
collection PubMed
description Human ADAR3 is a catalytically inactive member of the Adenosine Deaminase Acting on RNA (ADAR) protein family, whose active members catalyze A-to-I RNA editing in metazoans. Until now, the reasons for the catalytic incapability of ADAR3 has not been defined and its biological function rarely explored. Yet, its exclusive expression in the brain and involvement in learning and memory suggest a central role in the nervous system. Here we describe the engineering of a catalytically active ADAR3 enzyme using a combination of computational design and functional screening. Five mutations (A389V, V485I, E527Q, Q549R and Q733D) engender RNA deaminase in human ADAR3. By way of its catalytic activity, the ADAR3 pentamutant was used to identify potential binding targets for wild type ADAR3 in a human glioblastoma cell line. Novel ADAR3 binding sites discovered in this manner include the 3′-UTRs of the mRNAs encoding early growth response 1 (EGR1) and dual specificity phosphatase 1 (DUSP1); both known to be activity-dependent immediate early genes that respond to stimuli in the brain. Further studies reveal that the wild type ADAR3 protein can regulate transcript levels for DUSP1 and EGR1, suggesting a novel role ADAR3 may play in brain function.
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spelling pubmed-68467102019-11-18 RNA binding candidates for human ADAR3 from substrates of a gain of function mutant expressed in neuronal cells Wang, Yuru Chung, Dong hee Monteleone, Leanna R Li, Jie Chiang, Yao Toney, Michael D Beal, Peter A Nucleic Acids Res Nucleic Acid Enzymes Human ADAR3 is a catalytically inactive member of the Adenosine Deaminase Acting on RNA (ADAR) protein family, whose active members catalyze A-to-I RNA editing in metazoans. Until now, the reasons for the catalytic incapability of ADAR3 has not been defined and its biological function rarely explored. Yet, its exclusive expression in the brain and involvement in learning and memory suggest a central role in the nervous system. Here we describe the engineering of a catalytically active ADAR3 enzyme using a combination of computational design and functional screening. Five mutations (A389V, V485I, E527Q, Q549R and Q733D) engender RNA deaminase in human ADAR3. By way of its catalytic activity, the ADAR3 pentamutant was used to identify potential binding targets for wild type ADAR3 in a human glioblastoma cell line. Novel ADAR3 binding sites discovered in this manner include the 3′-UTRs of the mRNAs encoding early growth response 1 (EGR1) and dual specificity phosphatase 1 (DUSP1); both known to be activity-dependent immediate early genes that respond to stimuli in the brain. Further studies reveal that the wild type ADAR3 protein can regulate transcript levels for DUSP1 and EGR1, suggesting a novel role ADAR3 may play in brain function. Oxford University Press 2019-11-18 2019-09-25 /pmc/articles/PMC6846710/ /pubmed/31552420 http://dx.doi.org/10.1093/nar/gkz815 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Nucleic Acid Enzymes
Wang, Yuru
Chung, Dong hee
Monteleone, Leanna R
Li, Jie
Chiang, Yao
Toney, Michael D
Beal, Peter A
RNA binding candidates for human ADAR3 from substrates of a gain of function mutant expressed in neuronal cells
title RNA binding candidates for human ADAR3 from substrates of a gain of function mutant expressed in neuronal cells
title_full RNA binding candidates for human ADAR3 from substrates of a gain of function mutant expressed in neuronal cells
title_fullStr RNA binding candidates for human ADAR3 from substrates of a gain of function mutant expressed in neuronal cells
title_full_unstemmed RNA binding candidates for human ADAR3 from substrates of a gain of function mutant expressed in neuronal cells
title_short RNA binding candidates for human ADAR3 from substrates of a gain of function mutant expressed in neuronal cells
title_sort rna binding candidates for human adar3 from substrates of a gain of function mutant expressed in neuronal cells
topic Nucleic Acid Enzymes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6846710/
https://www.ncbi.nlm.nih.gov/pubmed/31552420
http://dx.doi.org/10.1093/nar/gkz815
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