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CRISPR/dual-FRET molecular beacon for sensitive live-cell imaging of non-repetitive genomic loci
Clustered regularly interspaced short palindromic repeats (CRISPR)-based genomic imaging systems predominantly rely on fluorescent protein reporters, which lack the optical properties essential for sensitive dynamic imaging. Here, we modified the CRISPR single-guide RNA (sgRNA) to carry two distinct...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6847002/ https://www.ncbi.nlm.nih.gov/pubmed/31504824 http://dx.doi.org/10.1093/nar/gkz752 |