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Increased CD83 expression of CD34-positive monocytes in donors during peripheral blood stem cell mobilization in humans
CD34-positive monocytes (CD34+mono) have recently been identified in grafts mobilized by granulocyte-colony stimulating factor. We analyzed transplant outcomes of 73 patients whose donor’s peripheral blood cells were cryopreserved during mobilization. CD34+mono was detected more frequently in male d...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6848192/ https://www.ncbi.nlm.nih.gov/pubmed/31712609 http://dx.doi.org/10.1038/s41598-019-53020-9 |
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author | Nakasone, Hideki Kikuchi, Misato Kawamura, Koji Akahoshi, Yu Sato, Miki Kawamura, Shunto Yoshino, Nozomu Takeshita, Junko Yoshimura, Kazuki Misaki, Yukiko Gomyo, Ayumi Tanihara, Aki Kusuda, Machiko Tamaki, Masaharu Kimura, Shun-ichi Kako, Shinichi Kanda, Yoshinobu |
author_facet | Nakasone, Hideki Kikuchi, Misato Kawamura, Koji Akahoshi, Yu Sato, Miki Kawamura, Shunto Yoshino, Nozomu Takeshita, Junko Yoshimura, Kazuki Misaki, Yukiko Gomyo, Ayumi Tanihara, Aki Kusuda, Machiko Tamaki, Masaharu Kimura, Shun-ichi Kako, Shinichi Kanda, Yoshinobu |
author_sort | Nakasone, Hideki |
collection | PubMed |
description | CD34-positive monocytes (CD34+mono) have recently been identified in grafts mobilized by granulocyte-colony stimulating factor. We analyzed transplant outcomes of 73 patients whose donor’s peripheral blood cells were cryopreserved during mobilization. CD34+mono was detected more frequently in male donors (67% vs. 40%, P = 0.03), while the detection of CD34+mono in donors was not associated with the patient background. Although there was no significant difference in overall survival in the whole cohort, the detection of CD34+mono in donors were significantly associated with a decreased risk of non-relapse mortality (HR 0.23, P = 0.035). Fatal infectious events tended to be less frequent in donors with CD34+mono. Gene expression profile analyses of CD34+mono in humans revealed that the expressions of pro-inflammatory cytokines like IL6, CCL3, IL8, VEGFA, and IL1A were elevated in CD34+mono, and those cytokines were enriched in the immune response, especially against infectious pathogens in the gene ontology analyses. In addition, the expression of CD83 was specifically increased in CD34+mono. It might play a role of antigen presentation in the immune network, leading in a clinical benefit against infections. Further investigations will be required to confirm the biological functions and clinical roles of CD34+mono in transplantation. |
format | Online Article Text |
id | pubmed-6848192 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-68481922019-11-19 Increased CD83 expression of CD34-positive monocytes in donors during peripheral blood stem cell mobilization in humans Nakasone, Hideki Kikuchi, Misato Kawamura, Koji Akahoshi, Yu Sato, Miki Kawamura, Shunto Yoshino, Nozomu Takeshita, Junko Yoshimura, Kazuki Misaki, Yukiko Gomyo, Ayumi Tanihara, Aki Kusuda, Machiko Tamaki, Masaharu Kimura, Shun-ichi Kako, Shinichi Kanda, Yoshinobu Sci Rep Article CD34-positive monocytes (CD34+mono) have recently been identified in grafts mobilized by granulocyte-colony stimulating factor. We analyzed transplant outcomes of 73 patients whose donor’s peripheral blood cells were cryopreserved during mobilization. CD34+mono was detected more frequently in male donors (67% vs. 40%, P = 0.03), while the detection of CD34+mono in donors was not associated with the patient background. Although there was no significant difference in overall survival in the whole cohort, the detection of CD34+mono in donors were significantly associated with a decreased risk of non-relapse mortality (HR 0.23, P = 0.035). Fatal infectious events tended to be less frequent in donors with CD34+mono. Gene expression profile analyses of CD34+mono in humans revealed that the expressions of pro-inflammatory cytokines like IL6, CCL3, IL8, VEGFA, and IL1A were elevated in CD34+mono, and those cytokines were enriched in the immune response, especially against infectious pathogens in the gene ontology analyses. In addition, the expression of CD83 was specifically increased in CD34+mono. It might play a role of antigen presentation in the immune network, leading in a clinical benefit against infections. Further investigations will be required to confirm the biological functions and clinical roles of CD34+mono in transplantation. Nature Publishing Group UK 2019-11-11 /pmc/articles/PMC6848192/ /pubmed/31712609 http://dx.doi.org/10.1038/s41598-019-53020-9 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Nakasone, Hideki Kikuchi, Misato Kawamura, Koji Akahoshi, Yu Sato, Miki Kawamura, Shunto Yoshino, Nozomu Takeshita, Junko Yoshimura, Kazuki Misaki, Yukiko Gomyo, Ayumi Tanihara, Aki Kusuda, Machiko Tamaki, Masaharu Kimura, Shun-ichi Kako, Shinichi Kanda, Yoshinobu Increased CD83 expression of CD34-positive monocytes in donors during peripheral blood stem cell mobilization in humans |
title | Increased CD83 expression of CD34-positive monocytes in donors during peripheral blood stem cell mobilization in humans |
title_full | Increased CD83 expression of CD34-positive monocytes in donors during peripheral blood stem cell mobilization in humans |
title_fullStr | Increased CD83 expression of CD34-positive monocytes in donors during peripheral blood stem cell mobilization in humans |
title_full_unstemmed | Increased CD83 expression of CD34-positive monocytes in donors during peripheral blood stem cell mobilization in humans |
title_short | Increased CD83 expression of CD34-positive monocytes in donors during peripheral blood stem cell mobilization in humans |
title_sort | increased cd83 expression of cd34-positive monocytes in donors during peripheral blood stem cell mobilization in humans |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6848192/ https://www.ncbi.nlm.nih.gov/pubmed/31712609 http://dx.doi.org/10.1038/s41598-019-53020-9 |
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