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Analysis of metabolomics associated with quality differences between room‐temperature‐ and low‐temperature‐stored litchi pulps

Studies on how temperature affects the postharvest quality of litchi have focused mainly on pericarp browning but rarely on the metabolites in postharvest litchi pulp. In this study, the differences in respiration rates, total soluble solid content, and titratable acid content demonstrated that room...

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Autores principales: Guo, Xiaomeng, Luo, Tao, Han, Dongmei, Wu, Zhenxian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6848819/
https://www.ncbi.nlm.nih.gov/pubmed/31763006
http://dx.doi.org/10.1002/fsn3.1208
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author Guo, Xiaomeng
Luo, Tao
Han, Dongmei
Wu, Zhenxian
author_facet Guo, Xiaomeng
Luo, Tao
Han, Dongmei
Wu, Zhenxian
author_sort Guo, Xiaomeng
collection PubMed
description Studies on how temperature affects the postharvest quality of litchi have focused mainly on pericarp browning but rarely on the metabolites in postharvest litchi pulp. In this study, the differences in respiration rates, total soluble solid content, and titratable acid content demonstrated that room and low temperatures have different effects on the quality of “Feizixiao” litchi pulp. UHPLC‐ESI‐QTOF‐MS/MS analysis was performed to compare the differentially expressed metabolites (DEMs) in litchi pulp after 8 days of storage at room temperature (RT‐8 d) with those in litchi pulp after 28 days of storage at low temperature (LT‐28 d). Nineteen carbohydrates (phosphohexoses, sorbitol, and mannose), fifteen acids, seven amino acids, nine energy metabolites and nucleotides, and six aliphatic and secondary metabolites were identified as common DEMs in RT‐8 d and LT‐28 d pulps. These findings indicated active fructose and mannose metabolism and increased catabolism of nicotinate, nicotinamide, alanine, aspartate, and glutamate. Four carbohydrates (mainly phosphohexoses), five acids, ten amino acids, three aliphatic and secondary metabolites, and one hormone were identified as unique DEMs in RT‐8 d pulp, the consumption of key metabolites in glycolysis and the tricarboxylic acid cycle, and accumulation of phenylalanine, tyrosine, and tryptophan. Active consumption of nucleotide metabolites and biosynthesis of aliphatics in LT‐28 d pulp were indicated by unique DEMs (eleven carbohydrates, four acids, seven amino acids, seven energy metabolites and nucleotides, and six aliphatic and secondary metabolites). These results provided an unambiguous metabolic fingerprint, thereby revealing how room and low temperatures differentially influenced the quality of litchi pulp.
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spelling pubmed-68488192019-11-22 Analysis of metabolomics associated with quality differences between room‐temperature‐ and low‐temperature‐stored litchi pulps Guo, Xiaomeng Luo, Tao Han, Dongmei Wu, Zhenxian Food Sci Nutr Original Research Studies on how temperature affects the postharvest quality of litchi have focused mainly on pericarp browning but rarely on the metabolites in postharvest litchi pulp. In this study, the differences in respiration rates, total soluble solid content, and titratable acid content demonstrated that room and low temperatures have different effects on the quality of “Feizixiao” litchi pulp. UHPLC‐ESI‐QTOF‐MS/MS analysis was performed to compare the differentially expressed metabolites (DEMs) in litchi pulp after 8 days of storage at room temperature (RT‐8 d) with those in litchi pulp after 28 days of storage at low temperature (LT‐28 d). Nineteen carbohydrates (phosphohexoses, sorbitol, and mannose), fifteen acids, seven amino acids, nine energy metabolites and nucleotides, and six aliphatic and secondary metabolites were identified as common DEMs in RT‐8 d and LT‐28 d pulps. These findings indicated active fructose and mannose metabolism and increased catabolism of nicotinate, nicotinamide, alanine, aspartate, and glutamate. Four carbohydrates (mainly phosphohexoses), five acids, ten amino acids, three aliphatic and secondary metabolites, and one hormone were identified as unique DEMs in RT‐8 d pulp, the consumption of key metabolites in glycolysis and the tricarboxylic acid cycle, and accumulation of phenylalanine, tyrosine, and tryptophan. Active consumption of nucleotide metabolites and biosynthesis of aliphatics in LT‐28 d pulp were indicated by unique DEMs (eleven carbohydrates, four acids, seven amino acids, seven energy metabolites and nucleotides, and six aliphatic and secondary metabolites). These results provided an unambiguous metabolic fingerprint, thereby revealing how room and low temperatures differentially influenced the quality of litchi pulp. John Wiley and Sons Inc. 2019-09-30 /pmc/articles/PMC6848819/ /pubmed/31763006 http://dx.doi.org/10.1002/fsn3.1208 Text en © 2019 The Authors. Food Science & Nutrition published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Guo, Xiaomeng
Luo, Tao
Han, Dongmei
Wu, Zhenxian
Analysis of metabolomics associated with quality differences between room‐temperature‐ and low‐temperature‐stored litchi pulps
title Analysis of metabolomics associated with quality differences between room‐temperature‐ and low‐temperature‐stored litchi pulps
title_full Analysis of metabolomics associated with quality differences between room‐temperature‐ and low‐temperature‐stored litchi pulps
title_fullStr Analysis of metabolomics associated with quality differences between room‐temperature‐ and low‐temperature‐stored litchi pulps
title_full_unstemmed Analysis of metabolomics associated with quality differences between room‐temperature‐ and low‐temperature‐stored litchi pulps
title_short Analysis of metabolomics associated with quality differences between room‐temperature‐ and low‐temperature‐stored litchi pulps
title_sort analysis of metabolomics associated with quality differences between room‐temperature‐ and low‐temperature‐stored litchi pulps
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6848819/
https://www.ncbi.nlm.nih.gov/pubmed/31763006
http://dx.doi.org/10.1002/fsn3.1208
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