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The influence of the extraction method on bioactivity of the root of Tetrastigma hemsleyanum
Tetrastigma hemsleyanum is traditionally used as a folk medicine and functional food in China. Its extracts have been confirmed to have many bioactivities. However, the effect of extracting temperature on its bioactivity has not been reported. In this research, the total flavonoids content (TFC), to...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6848824/ https://www.ncbi.nlm.nih.gov/pubmed/31763013 http://dx.doi.org/10.1002/fsn3.1221 |
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author | Liu, Yangyang Ye, Xiang Li, Yonglu Chu, Qiang Yu, Lushuang Chen, Wen Jia, Ruoyi Jiang, Yong Zheng, Xiaodong |
author_facet | Liu, Yangyang Ye, Xiang Li, Yonglu Chu, Qiang Yu, Lushuang Chen, Wen Jia, Ruoyi Jiang, Yong Zheng, Xiaodong |
author_sort | Liu, Yangyang |
collection | PubMed |
description | Tetrastigma hemsleyanum is traditionally used as a folk medicine and functional food in China. Its extracts have been confirmed to have many bioactivities. However, the effect of extracting temperature on its bioactivity has not been reported. In this research, the total flavonoids content (TFC), total polyphenol content (TPC), antiproliferative, antioxidant, and anti‐inflammatory activities of ethanol extracts and water extracts (extracted at 55, 70, 85, and 100°C) were observed. The results indicated that ethanol extracts showed better antioxidant activity with DPPH EC(50) of 504.1 ± 3.8 μg/ml and ABTS EC(50) of 851.4 ± 3.9 μg/ml. A better antiproliferative activity on HepG2, PC12, Caco‐2, and Hela cells was observed on ethanol extracts. The results of anti‐inflammatory activities also indicated that all of the extracts can reduce the NO production of LPS‐stimulated macrophage with dose‐independent manner. In summary, the results showed that the antiproliferative, antioxidant, and anti‐inflammatory activities of water extracts decreased with the increasing temperature to some extent. |
format | Online Article Text |
id | pubmed-6848824 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-68488242019-11-22 The influence of the extraction method on bioactivity of the root of Tetrastigma hemsleyanum Liu, Yangyang Ye, Xiang Li, Yonglu Chu, Qiang Yu, Lushuang Chen, Wen Jia, Ruoyi Jiang, Yong Zheng, Xiaodong Food Sci Nutr Original Research Tetrastigma hemsleyanum is traditionally used as a folk medicine and functional food in China. Its extracts have been confirmed to have many bioactivities. However, the effect of extracting temperature on its bioactivity has not been reported. In this research, the total flavonoids content (TFC), total polyphenol content (TPC), antiproliferative, antioxidant, and anti‐inflammatory activities of ethanol extracts and water extracts (extracted at 55, 70, 85, and 100°C) were observed. The results indicated that ethanol extracts showed better antioxidant activity with DPPH EC(50) of 504.1 ± 3.8 μg/ml and ABTS EC(50) of 851.4 ± 3.9 μg/ml. A better antiproliferative activity on HepG2, PC12, Caco‐2, and Hela cells was observed on ethanol extracts. The results of anti‐inflammatory activities also indicated that all of the extracts can reduce the NO production of LPS‐stimulated macrophage with dose‐independent manner. In summary, the results showed that the antiproliferative, antioxidant, and anti‐inflammatory activities of water extracts decreased with the increasing temperature to some extent. John Wiley and Sons Inc. 2019-10-21 /pmc/articles/PMC6848824/ /pubmed/31763013 http://dx.doi.org/10.1002/fsn3.1221 Text en © 2019 The Authors. Food Science & Nutrition published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Liu, Yangyang Ye, Xiang Li, Yonglu Chu, Qiang Yu, Lushuang Chen, Wen Jia, Ruoyi Jiang, Yong Zheng, Xiaodong The influence of the extraction method on bioactivity of the root of Tetrastigma hemsleyanum |
title | The influence of the extraction method on bioactivity of the root of Tetrastigma hemsleyanum
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title_full | The influence of the extraction method on bioactivity of the root of Tetrastigma hemsleyanum
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title_fullStr | The influence of the extraction method on bioactivity of the root of Tetrastigma hemsleyanum
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title_full_unstemmed | The influence of the extraction method on bioactivity of the root of Tetrastigma hemsleyanum
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title_short | The influence of the extraction method on bioactivity of the root of Tetrastigma hemsleyanum
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title_sort | influence of the extraction method on bioactivity of the root of tetrastigma hemsleyanum |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6848824/ https://www.ncbi.nlm.nih.gov/pubmed/31763013 http://dx.doi.org/10.1002/fsn3.1221 |
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