Cargando…
Age and Sex Influence the Neuro-inflammatory Response to a Peripheral Acute LPS Challenge
Aging is associated with an exaggerated response to peripheral inflammatory challenges together with behavioral and cognitive deficits. Studies considering both age and sex remain limited, despite sex dimorphism of astrocytes and microglial cells is largely recognized. To fill this knowledge gap, we...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6848890/ https://www.ncbi.nlm.nih.gov/pubmed/31749696 http://dx.doi.org/10.3389/fnagi.2019.00299 |
_version_ | 1783469098100850688 |
---|---|
author | Murtaj, Valentina Belloli, Sara Di Grigoli, Giuseppe Pannese, Maria Ballarini, Elisa Rodriguez-Menendez, Virginia Marmiroli, Paola Cappelli, Andrea Masiello, Valeria Monterisi, Cristina Bellelli, Giuseppe Panina-Bordignon, Paola Moresco, Rosa Maria |
author_facet | Murtaj, Valentina Belloli, Sara Di Grigoli, Giuseppe Pannese, Maria Ballarini, Elisa Rodriguez-Menendez, Virginia Marmiroli, Paola Cappelli, Andrea Masiello, Valeria Monterisi, Cristina Bellelli, Giuseppe Panina-Bordignon, Paola Moresco, Rosa Maria |
author_sort | Murtaj, Valentina |
collection | PubMed |
description | Aging is associated with an exaggerated response to peripheral inflammatory challenges together with behavioral and cognitive deficits. Studies considering both age and sex remain limited, despite sex dimorphism of astrocytes and microglial cells is largely recognized. To fill this knowledge gap, we investigated the effect of a single intraperitoneal lipopolysaccharide (LPS) administration in adult and aged mice. We assessed the expression of different inflammatory mediators, and the microglial response through binding of [(18)F]-VC701 tracer to translocator protein (TSPO) receptors in the male and female brain. Aged female brain showed a higher pro-inflammatory response to LPS compared to adult female and to aged male, as revealed by ex vivo binding to TSPO receptors and pro-inflammatory mediator transcript levels. The highest astroglial reaction was observed in the brain of aged females. Differently to the other groups of animals, in aged males LPS challenge did not affect transcription of triggering receptor expressed on myeloid cells 2 (TREM2). In conclusion, our study shows that in the mouse’s brain the neuro-inflammatory response to an acute peripheral insult is sex- and age-dependent. Moreover, our results might set the basis for further studies aimed at identifying sex-related targets involved in the modulation of the aberrant neuro-inflammatory response that characterizes aging. This knowledge could be relevant for the treatment of conditions such as delirium and dementia. |
format | Online Article Text |
id | pubmed-6848890 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-68488902019-11-20 Age and Sex Influence the Neuro-inflammatory Response to a Peripheral Acute LPS Challenge Murtaj, Valentina Belloli, Sara Di Grigoli, Giuseppe Pannese, Maria Ballarini, Elisa Rodriguez-Menendez, Virginia Marmiroli, Paola Cappelli, Andrea Masiello, Valeria Monterisi, Cristina Bellelli, Giuseppe Panina-Bordignon, Paola Moresco, Rosa Maria Front Aging Neurosci Neuroscience Aging is associated with an exaggerated response to peripheral inflammatory challenges together with behavioral and cognitive deficits. Studies considering both age and sex remain limited, despite sex dimorphism of astrocytes and microglial cells is largely recognized. To fill this knowledge gap, we investigated the effect of a single intraperitoneal lipopolysaccharide (LPS) administration in adult and aged mice. We assessed the expression of different inflammatory mediators, and the microglial response through binding of [(18)F]-VC701 tracer to translocator protein (TSPO) receptors in the male and female brain. Aged female brain showed a higher pro-inflammatory response to LPS compared to adult female and to aged male, as revealed by ex vivo binding to TSPO receptors and pro-inflammatory mediator transcript levels. The highest astroglial reaction was observed in the brain of aged females. Differently to the other groups of animals, in aged males LPS challenge did not affect transcription of triggering receptor expressed on myeloid cells 2 (TREM2). In conclusion, our study shows that in the mouse’s brain the neuro-inflammatory response to an acute peripheral insult is sex- and age-dependent. Moreover, our results might set the basis for further studies aimed at identifying sex-related targets involved in the modulation of the aberrant neuro-inflammatory response that characterizes aging. This knowledge could be relevant for the treatment of conditions such as delirium and dementia. Frontiers Media S.A. 2019-11-05 /pmc/articles/PMC6848890/ /pubmed/31749696 http://dx.doi.org/10.3389/fnagi.2019.00299 Text en Copyright © 2019 Murtaj, Belloli, Di Grigoli, Pannese, Ballarini, Rodriguez-Menendez, Marmiroli, Cappelli, Masiello, Monterisi, Bellelli, Panina-Bordignon and Moresco. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Murtaj, Valentina Belloli, Sara Di Grigoli, Giuseppe Pannese, Maria Ballarini, Elisa Rodriguez-Menendez, Virginia Marmiroli, Paola Cappelli, Andrea Masiello, Valeria Monterisi, Cristina Bellelli, Giuseppe Panina-Bordignon, Paola Moresco, Rosa Maria Age and Sex Influence the Neuro-inflammatory Response to a Peripheral Acute LPS Challenge |
title | Age and Sex Influence the Neuro-inflammatory Response to a Peripheral Acute LPS Challenge |
title_full | Age and Sex Influence the Neuro-inflammatory Response to a Peripheral Acute LPS Challenge |
title_fullStr | Age and Sex Influence the Neuro-inflammatory Response to a Peripheral Acute LPS Challenge |
title_full_unstemmed | Age and Sex Influence the Neuro-inflammatory Response to a Peripheral Acute LPS Challenge |
title_short | Age and Sex Influence the Neuro-inflammatory Response to a Peripheral Acute LPS Challenge |
title_sort | age and sex influence the neuro-inflammatory response to a peripheral acute lps challenge |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6848890/ https://www.ncbi.nlm.nih.gov/pubmed/31749696 http://dx.doi.org/10.3389/fnagi.2019.00299 |
work_keys_str_mv | AT murtajvalentina ageandsexinfluencetheneuroinflammatoryresponsetoaperipheralacutelpschallenge AT bellolisara ageandsexinfluencetheneuroinflammatoryresponsetoaperipheralacutelpschallenge AT digrigoligiuseppe ageandsexinfluencetheneuroinflammatoryresponsetoaperipheralacutelpschallenge AT pannesemaria ageandsexinfluencetheneuroinflammatoryresponsetoaperipheralacutelpschallenge AT ballarinielisa ageandsexinfluencetheneuroinflammatoryresponsetoaperipheralacutelpschallenge AT rodriguezmenendezvirginia ageandsexinfluencetheneuroinflammatoryresponsetoaperipheralacutelpschallenge AT marmirolipaola ageandsexinfluencetheneuroinflammatoryresponsetoaperipheralacutelpschallenge AT cappelliandrea ageandsexinfluencetheneuroinflammatoryresponsetoaperipheralacutelpschallenge AT masiellovaleria ageandsexinfluencetheneuroinflammatoryresponsetoaperipheralacutelpschallenge AT monterisicristina ageandsexinfluencetheneuroinflammatoryresponsetoaperipheralacutelpschallenge AT bellelligiuseppe ageandsexinfluencetheneuroinflammatoryresponsetoaperipheralacutelpschallenge AT paninabordignonpaola ageandsexinfluencetheneuroinflammatoryresponsetoaperipheralacutelpschallenge AT morescorosamaria ageandsexinfluencetheneuroinflammatoryresponsetoaperipheralacutelpschallenge |