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Comparative evaluation of two commercial ELISA kits for detection of antibodies against Akabane virus in cattle serum
BACKGROUND: Akabane disease (AD), a barrier to international trade for endemic areas with far economic impact on the countries, is caused by Akabane virus (AKAV). Commercial enzyme-linked immunosorbent assay (ELISA) is a commonly used diagnostic technique for AKAV infection, including the IDEXX and...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6849277/ https://www.ncbi.nlm.nih.gov/pubmed/31711494 http://dx.doi.org/10.1186/s12917-019-2156-6 |
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author | Li, Xiaolin Jing, Hongli Liu, Xiaofei Wang, Qin Qiu, Songyin Liu, Dandan Wu, Shaoqiang Lin, Xiangmei |
author_facet | Li, Xiaolin Jing, Hongli Liu, Xiaofei Wang, Qin Qiu, Songyin Liu, Dandan Wu, Shaoqiang Lin, Xiangmei |
author_sort | Li, Xiaolin |
collection | PubMed |
description | BACKGROUND: Akabane disease (AD), a barrier to international trade for endemic areas with far economic impact on the countries, is caused by Akabane virus (AKAV). Commercial enzyme-linked immunosorbent assay (ELISA) is a commonly used diagnostic technique for AKAV infection, including the IDEXX and IDVET ELISA kits. However, the comparative evaluation of the IDEXX and IDVET ELISA kits has not been published. The object of this study was to evaluate the test performance of the two commercial ELISA kits in detecting serum anti-AKAV antibodies in cattle. RESULTS: With virus neutralization test (VNT) as the “relative gold standard”, the diagnostic sensitivity (DSe) was 80.39% (123/153) and 93.46% (143/153) for the IDEXX and IDVET ELISA kit, when suspect samples were included. The diagnostic specificity (DSp) for the IDEXX and IDVET ELISA kit was 93.48% (502/537) and 82.31% (442/537), respectively. CONCLUSION: Both of the tested ELISA kits could be applied to detect antibodies against AKAV in cattle serum. The IDVET ELISA kit had a higher DSe. The IDEXX ELISA kit possessed the higher DSp. These results have important implications if the kits are used to screen herds or individual cattle in surveillance programs, or at border crossings for import-export inspection and quarantine. |
format | Online Article Text |
id | pubmed-6849277 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-68492772019-11-15 Comparative evaluation of two commercial ELISA kits for detection of antibodies against Akabane virus in cattle serum Li, Xiaolin Jing, Hongli Liu, Xiaofei Wang, Qin Qiu, Songyin Liu, Dandan Wu, Shaoqiang Lin, Xiangmei BMC Vet Res Methodology Article BACKGROUND: Akabane disease (AD), a barrier to international trade for endemic areas with far economic impact on the countries, is caused by Akabane virus (AKAV). Commercial enzyme-linked immunosorbent assay (ELISA) is a commonly used diagnostic technique for AKAV infection, including the IDEXX and IDVET ELISA kits. However, the comparative evaluation of the IDEXX and IDVET ELISA kits has not been published. The object of this study was to evaluate the test performance of the two commercial ELISA kits in detecting serum anti-AKAV antibodies in cattle. RESULTS: With virus neutralization test (VNT) as the “relative gold standard”, the diagnostic sensitivity (DSe) was 80.39% (123/153) and 93.46% (143/153) for the IDEXX and IDVET ELISA kit, when suspect samples were included. The diagnostic specificity (DSp) for the IDEXX and IDVET ELISA kit was 93.48% (502/537) and 82.31% (442/537), respectively. CONCLUSION: Both of the tested ELISA kits could be applied to detect antibodies against AKAV in cattle serum. The IDVET ELISA kit had a higher DSe. The IDEXX ELISA kit possessed the higher DSp. These results have important implications if the kits are used to screen herds or individual cattle in surveillance programs, or at border crossings for import-export inspection and quarantine. BioMed Central 2019-11-11 /pmc/articles/PMC6849277/ /pubmed/31711494 http://dx.doi.org/10.1186/s12917-019-2156-6 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Methodology Article Li, Xiaolin Jing, Hongli Liu, Xiaofei Wang, Qin Qiu, Songyin Liu, Dandan Wu, Shaoqiang Lin, Xiangmei Comparative evaluation of two commercial ELISA kits for detection of antibodies against Akabane virus in cattle serum |
title | Comparative evaluation of two commercial ELISA kits for detection of antibodies against Akabane virus in cattle serum |
title_full | Comparative evaluation of two commercial ELISA kits for detection of antibodies against Akabane virus in cattle serum |
title_fullStr | Comparative evaluation of two commercial ELISA kits for detection of antibodies against Akabane virus in cattle serum |
title_full_unstemmed | Comparative evaluation of two commercial ELISA kits for detection of antibodies against Akabane virus in cattle serum |
title_short | Comparative evaluation of two commercial ELISA kits for detection of antibodies against Akabane virus in cattle serum |
title_sort | comparative evaluation of two commercial elisa kits for detection of antibodies against akabane virus in cattle serum |
topic | Methodology Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6849277/ https://www.ncbi.nlm.nih.gov/pubmed/31711494 http://dx.doi.org/10.1186/s12917-019-2156-6 |
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