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Hormonal induction of spermiation in a Eurasian bufonid (Epidalea calamita)

BACKGROUND: Amphibian diversity is declining at an alarming rate due to habitat loss, invasive species, climate change and diseases. Captive assurance colonies have been established for some species at risk; however, many species do not breed well in captivity and the development of assisted reprodu...

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Autores principales: Arregui, Lucía, Diaz-Diaz, Sergio, Alonso-López, Elia, Kouba, Andrew J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6849308/
https://www.ncbi.nlm.nih.gov/pubmed/31711511
http://dx.doi.org/10.1186/s12958-019-0537-0
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author Arregui, Lucía
Diaz-Diaz, Sergio
Alonso-López, Elia
Kouba, Andrew J.
author_facet Arregui, Lucía
Diaz-Diaz, Sergio
Alonso-López, Elia
Kouba, Andrew J.
author_sort Arregui, Lucía
collection PubMed
description BACKGROUND: Amphibian diversity is declining at an alarming rate due to habitat loss, invasive species, climate change and diseases. Captive assurance colonies have been established for some species at risk; however, many species do not breed well in captivity and the development of assisted reproductive technologies (ART) is critical to help sustain genetic diversity. To date, the majority of the work has been accomplished in species from the American continent and Australia, and there is a need to address similar breeding challenges in Eurasian and African species of amphibians. METHODS: The aim of this study was to develop a hormone protocol for stimulation of spermiation in Epidalea calamita as a model for Eurasian bufonids. Hence, the effect on sperm production and quality of three doses of chorionic gonadotropin hormone (5, 10 and 15 IU hCG/g) over time (1 to 24 h) was evaluated. In addition, cold storage (at 5 °C) of sperm for 24 and 48 h and three frequencies for hormonal treatment (weekly, biweekly and monthly) were examined. RESULTS: Hormone concentrations of 10 or 15 IU of hCG induced spermiation in 100% of males and produced sperm of comparable quality, while 5 IU hCG stimulated spermiation in only 40% of males. Total motility peaked between 1 to 4 h post-treatment with 10 IU hCG, whereas treatment with 15 IU hCG peaked between 2 to 6 h. After 24 h of cold storage total motility dropped by 20% and forward motility dropped by 10% for both the 10 and 15 IU treatments. Weekly hormone administration resulted in higher variation between trials in all motility parameters and a lower overall Total Motility and Forward Movement. Furthermore, the effect of exogenous hormone treatment overlapped between the last two trials in the weekly frequency. Sperm concentration was higher in the first trial for all frequencies but showed no differences among other trials. CONCLUSIONS: Overall, these results show that hormone concentration, time after treatment, frequency of hormone treatment and cold storage should be borne in mind when developing a hormone stimulation protocol for Eurasian amphibian species.
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spelling pubmed-68493082019-11-15 Hormonal induction of spermiation in a Eurasian bufonid (Epidalea calamita) Arregui, Lucía Diaz-Diaz, Sergio Alonso-López, Elia Kouba, Andrew J. Reprod Biol Endocrinol Research BACKGROUND: Amphibian diversity is declining at an alarming rate due to habitat loss, invasive species, climate change and diseases. Captive assurance colonies have been established for some species at risk; however, many species do not breed well in captivity and the development of assisted reproductive technologies (ART) is critical to help sustain genetic diversity. To date, the majority of the work has been accomplished in species from the American continent and Australia, and there is a need to address similar breeding challenges in Eurasian and African species of amphibians. METHODS: The aim of this study was to develop a hormone protocol for stimulation of spermiation in Epidalea calamita as a model for Eurasian bufonids. Hence, the effect on sperm production and quality of three doses of chorionic gonadotropin hormone (5, 10 and 15 IU hCG/g) over time (1 to 24 h) was evaluated. In addition, cold storage (at 5 °C) of sperm for 24 and 48 h and three frequencies for hormonal treatment (weekly, biweekly and monthly) were examined. RESULTS: Hormone concentrations of 10 or 15 IU of hCG induced spermiation in 100% of males and produced sperm of comparable quality, while 5 IU hCG stimulated spermiation in only 40% of males. Total motility peaked between 1 to 4 h post-treatment with 10 IU hCG, whereas treatment with 15 IU hCG peaked between 2 to 6 h. After 24 h of cold storage total motility dropped by 20% and forward motility dropped by 10% for both the 10 and 15 IU treatments. Weekly hormone administration resulted in higher variation between trials in all motility parameters and a lower overall Total Motility and Forward Movement. Furthermore, the effect of exogenous hormone treatment overlapped between the last two trials in the weekly frequency. Sperm concentration was higher in the first trial for all frequencies but showed no differences among other trials. CONCLUSIONS: Overall, these results show that hormone concentration, time after treatment, frequency of hormone treatment and cold storage should be borne in mind when developing a hormone stimulation protocol for Eurasian amphibian species. BioMed Central 2019-11-11 /pmc/articles/PMC6849308/ /pubmed/31711511 http://dx.doi.org/10.1186/s12958-019-0537-0 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Arregui, Lucía
Diaz-Diaz, Sergio
Alonso-López, Elia
Kouba, Andrew J.
Hormonal induction of spermiation in a Eurasian bufonid (Epidalea calamita)
title Hormonal induction of spermiation in a Eurasian bufonid (Epidalea calamita)
title_full Hormonal induction of spermiation in a Eurasian bufonid (Epidalea calamita)
title_fullStr Hormonal induction of spermiation in a Eurasian bufonid (Epidalea calamita)
title_full_unstemmed Hormonal induction of spermiation in a Eurasian bufonid (Epidalea calamita)
title_short Hormonal induction of spermiation in a Eurasian bufonid (Epidalea calamita)
title_sort hormonal induction of spermiation in a eurasian bufonid (epidalea calamita)
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6849308/
https://www.ncbi.nlm.nih.gov/pubmed/31711511
http://dx.doi.org/10.1186/s12958-019-0537-0
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