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Hydrocarbon‐degrading bacteria in deep‐water subarctic sediments (Faroe‐Shetland Channel)

AIMS: The aim of this study was the baseline description of oil‐degrading sediment bacteria along a depth transect in the Faroe‐Shetland Channel (FSC) and the identification of biomarker taxa for the detection of oil contamination in FSC sediments. METHODS AND RESULTS: Oil‐degrading sediment bacteri...

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Autores principales: Gontikaki, E., Potts, L.D., Anderson, J.A., Witte, U.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6849767/
https://www.ncbi.nlm.nih.gov/pubmed/29928773
http://dx.doi.org/10.1111/jam.14030
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author Gontikaki, E.
Potts, L.D.
Anderson, J.A.
Witte, U.
author_facet Gontikaki, E.
Potts, L.D.
Anderson, J.A.
Witte, U.
author_sort Gontikaki, E.
collection PubMed
description AIMS: The aim of this study was the baseline description of oil‐degrading sediment bacteria along a depth transect in the Faroe‐Shetland Channel (FSC) and the identification of biomarker taxa for the detection of oil contamination in FSC sediments. METHODS AND RESULTS: Oil‐degrading sediment bacteria from 135, 500 and 1000 m were enriched in cultures with crude oil as the sole carbon source (at 12, 5 and 0°C respectively). The enriched communities were studied using culture‐dependent and culture‐independent (clone libraries) techniques. Isolated bacterial strains were tested for hydrocarbon degradation capability. Bacterial isolates included well‐known oil‐degrading taxa and several that are reported in that capacity for the first time (Sulfitobacter, Ahrensia, Belliella, Chryseobacterium). The orders Oceanospirillales and Alteromonadales dominated clone libraries in all stations but significant differences occurred at genus level particularly between the shallow and the deep, cold‐water stations. Alcanivorax constituted 64% of clones at FSC135 but was absent at deeper stations. Pseudoalteromonas and Oleispira dominated the bacterial community at 500 and 1000 m. CONCLUSIONS: The genus Oleispira emerged as a major player in the early stages of crude oil degradation in deep‐sea sediments of the FSC particularly at subzero temperatures. This finding is offering a direction for future research into biomonitoring tools for the detection of low levels of crude oil contamination in the deep FSC, and possibly high latitude cold waters in general. SIGNIFICANCE AND IMPACT OF THE STUDY: Oil and gas exploration in the FSC occurs at depths >1000 m but baseline environmental data necessary for the assessment of ecosystem recovery to prespill conditions in the event of an oil spill are lacking. This study will contribute to our ability to assess the impact of oil release in the FSC and guide the direction of bioremediation strategies tailored to the area.
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spelling pubmed-68497672019-11-15 Hydrocarbon‐degrading bacteria in deep‐water subarctic sediments (Faroe‐Shetland Channel) Gontikaki, E. Potts, L.D. Anderson, J.A. Witte, U. J Appl Microbiol Original Articles AIMS: The aim of this study was the baseline description of oil‐degrading sediment bacteria along a depth transect in the Faroe‐Shetland Channel (FSC) and the identification of biomarker taxa for the detection of oil contamination in FSC sediments. METHODS AND RESULTS: Oil‐degrading sediment bacteria from 135, 500 and 1000 m were enriched in cultures with crude oil as the sole carbon source (at 12, 5 and 0°C respectively). The enriched communities were studied using culture‐dependent and culture‐independent (clone libraries) techniques. Isolated bacterial strains were tested for hydrocarbon degradation capability. Bacterial isolates included well‐known oil‐degrading taxa and several that are reported in that capacity for the first time (Sulfitobacter, Ahrensia, Belliella, Chryseobacterium). The orders Oceanospirillales and Alteromonadales dominated clone libraries in all stations but significant differences occurred at genus level particularly between the shallow and the deep, cold‐water stations. Alcanivorax constituted 64% of clones at FSC135 but was absent at deeper stations. Pseudoalteromonas and Oleispira dominated the bacterial community at 500 and 1000 m. CONCLUSIONS: The genus Oleispira emerged as a major player in the early stages of crude oil degradation in deep‐sea sediments of the FSC particularly at subzero temperatures. This finding is offering a direction for future research into biomonitoring tools for the detection of low levels of crude oil contamination in the deep FSC, and possibly high latitude cold waters in general. SIGNIFICANCE AND IMPACT OF THE STUDY: Oil and gas exploration in the FSC occurs at depths >1000 m but baseline environmental data necessary for the assessment of ecosystem recovery to prespill conditions in the event of an oil spill are lacking. This study will contribute to our ability to assess the impact of oil release in the FSC and guide the direction of bioremediation strategies tailored to the area. John Wiley and Sons Inc. 2018-07-24 2018-10 /pmc/articles/PMC6849767/ /pubmed/29928773 http://dx.doi.org/10.1111/jam.14030 Text en © 2018 The Authors. Journal of Applied Microbiology published by John Wiley & Sons Ltd on behalf of The Society for Applied Microbiology. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Gontikaki, E.
Potts, L.D.
Anderson, J.A.
Witte, U.
Hydrocarbon‐degrading bacteria in deep‐water subarctic sediments (Faroe‐Shetland Channel)
title Hydrocarbon‐degrading bacteria in deep‐water subarctic sediments (Faroe‐Shetland Channel)
title_full Hydrocarbon‐degrading bacteria in deep‐water subarctic sediments (Faroe‐Shetland Channel)
title_fullStr Hydrocarbon‐degrading bacteria in deep‐water subarctic sediments (Faroe‐Shetland Channel)
title_full_unstemmed Hydrocarbon‐degrading bacteria in deep‐water subarctic sediments (Faroe‐Shetland Channel)
title_short Hydrocarbon‐degrading bacteria in deep‐water subarctic sediments (Faroe‐Shetland Channel)
title_sort hydrocarbon‐degrading bacteria in deep‐water subarctic sediments (faroe‐shetland channel)
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6849767/
https://www.ncbi.nlm.nih.gov/pubmed/29928773
http://dx.doi.org/10.1111/jam.14030
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