Cargando…

Involvement of sweet pepper CaLOX2 in jasmonate‐dependent induced defence against Western flower thrips

Insect herbivory can seriously hinder plant performance and reduce crop yield. Thrips are minute cell‐content‐feeding insects that are important vectors of viral plant pathogens, and are serious crop pests. We investigated the role of a sweet pepper (Capsicum annuum) lipoxygenase gene, CaLOX2, in th...

Descripción completa

Detalles Bibliográficos
Autores principales: Sarde, Sandeep J, Bouwmeester, Klaas, Venegas‐Molina, Jhon, David, Anja, Boland, Wilhelm, Dicke, Marcel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6850143/
https://www.ncbi.nlm.nih.gov/pubmed/30450727
http://dx.doi.org/10.1111/jipb.12742
_version_ 1783469357644382208
author Sarde, Sandeep J
Bouwmeester, Klaas
Venegas‐Molina, Jhon
David, Anja
Boland, Wilhelm
Dicke, Marcel
author_facet Sarde, Sandeep J
Bouwmeester, Klaas
Venegas‐Molina, Jhon
David, Anja
Boland, Wilhelm
Dicke, Marcel
author_sort Sarde, Sandeep J
collection PubMed
description Insect herbivory can seriously hinder plant performance and reduce crop yield. Thrips are minute cell‐content‐feeding insects that are important vectors of viral plant pathogens, and are serious crop pests. We investigated the role of a sweet pepper (Capsicum annuum) lipoxygenase gene, CaLOX2, in the defense of pepper plants against Western flower thrips (Frankliniella occidentalis). This was done through a combination of in‐silico, transcriptional, behavioral and chemical analyses. Our data show that CaLOX2 is involved in jasmonic acid (JA) biosynthesis and mediates plant resistance. Expression of the JA‐related marker genes, CaLOX2 and CaPIN II, was induced by thrips feeding. Silencing of CaLOX2 in pepper plants through virus‐induced gene silencing (VIGS) resulted in low levels of CaLOX2 transcripts, as well as significant reduction in the accumulation of JA, and its derivatives, upon thrips feeding compared to control plants. CaLOX2‐silenced pepper plants exhibited enhanced susceptibility to thrips. This indicates that CaLOX2 mediates JA‐dependent signaling, resulting in defense against thrips. Furthermore, exogenous application of JA to pepper plants increased plant resistance to thrips, constrained thrips population development and made plants less attractive to thrips. Thus, a multidisciplinary approach shows that an intact lipoxygenase pathway mediates various components of sweet pepper defense against F. occidentalis.
format Online
Article
Text
id pubmed-6850143
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-68501432019-11-15 Involvement of sweet pepper CaLOX2 in jasmonate‐dependent induced defence against Western flower thrips Sarde, Sandeep J Bouwmeester, Klaas Venegas‐Molina, Jhon David, Anja Boland, Wilhelm Dicke, Marcel J Integr Plant Biol Plant‐biotic Interactions Insect herbivory can seriously hinder plant performance and reduce crop yield. Thrips are minute cell‐content‐feeding insects that are important vectors of viral plant pathogens, and are serious crop pests. We investigated the role of a sweet pepper (Capsicum annuum) lipoxygenase gene, CaLOX2, in the defense of pepper plants against Western flower thrips (Frankliniella occidentalis). This was done through a combination of in‐silico, transcriptional, behavioral and chemical analyses. Our data show that CaLOX2 is involved in jasmonic acid (JA) biosynthesis and mediates plant resistance. Expression of the JA‐related marker genes, CaLOX2 and CaPIN II, was induced by thrips feeding. Silencing of CaLOX2 in pepper plants through virus‐induced gene silencing (VIGS) resulted in low levels of CaLOX2 transcripts, as well as significant reduction in the accumulation of JA, and its derivatives, upon thrips feeding compared to control plants. CaLOX2‐silenced pepper plants exhibited enhanced susceptibility to thrips. This indicates that CaLOX2 mediates JA‐dependent signaling, resulting in defense against thrips. Furthermore, exogenous application of JA to pepper plants increased plant resistance to thrips, constrained thrips population development and made plants less attractive to thrips. Thus, a multidisciplinary approach shows that an intact lipoxygenase pathway mediates various components of sweet pepper defense against F. occidentalis. John Wiley and Sons Inc. 2019-02-27 2019-10 /pmc/articles/PMC6850143/ /pubmed/30450727 http://dx.doi.org/10.1111/jipb.12742 Text en © 2018 The Authors. Journal of Integrative Plant Biology Published by John Wiley & Sons Australia, Ltd on behalf of Institute of Botany, Chinese Academy of Sciences This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Plant‐biotic Interactions
Sarde, Sandeep J
Bouwmeester, Klaas
Venegas‐Molina, Jhon
David, Anja
Boland, Wilhelm
Dicke, Marcel
Involvement of sweet pepper CaLOX2 in jasmonate‐dependent induced defence against Western flower thrips
title Involvement of sweet pepper CaLOX2 in jasmonate‐dependent induced defence against Western flower thrips
title_full Involvement of sweet pepper CaLOX2 in jasmonate‐dependent induced defence against Western flower thrips
title_fullStr Involvement of sweet pepper CaLOX2 in jasmonate‐dependent induced defence against Western flower thrips
title_full_unstemmed Involvement of sweet pepper CaLOX2 in jasmonate‐dependent induced defence against Western flower thrips
title_short Involvement of sweet pepper CaLOX2 in jasmonate‐dependent induced defence against Western flower thrips
title_sort involvement of sweet pepper calox2 in jasmonate‐dependent induced defence against western flower thrips
topic Plant‐biotic Interactions
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6850143/
https://www.ncbi.nlm.nih.gov/pubmed/30450727
http://dx.doi.org/10.1111/jipb.12742
work_keys_str_mv AT sardesandeepj involvementofsweetpeppercalox2injasmonatedependentinduceddefenceagainstwesternflowerthrips
AT bouwmeesterklaas involvementofsweetpeppercalox2injasmonatedependentinduceddefenceagainstwesternflowerthrips
AT venegasmolinajhon involvementofsweetpeppercalox2injasmonatedependentinduceddefenceagainstwesternflowerthrips
AT davidanja involvementofsweetpeppercalox2injasmonatedependentinduceddefenceagainstwesternflowerthrips
AT bolandwilhelm involvementofsweetpeppercalox2injasmonatedependentinduceddefenceagainstwesternflowerthrips
AT dickemarcel involvementofsweetpeppercalox2injasmonatedependentinduceddefenceagainstwesternflowerthrips