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A multi‐level response to DNA damage induced by aluminium
Aluminium (Al) ions are one of the primary growth‐limiting factors for plants on acid soils, globally restricting agriculture. Despite its impact, little is known about Al action in planta. Earlier work has indicated that, among other effects, Al induces DNA damage. However, the loss of major DNA da...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6850279/ https://www.ncbi.nlm.nih.gov/pubmed/30657222 http://dx.doi.org/10.1111/tpj.14231 |
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author | Chen, Poyu Sjogren, Caroline A. Larsen, Paul B. Schnittger, Arp |
author_facet | Chen, Poyu Sjogren, Caroline A. Larsen, Paul B. Schnittger, Arp |
author_sort | Chen, Poyu |
collection | PubMed |
description | Aluminium (Al) ions are one of the primary growth‐limiting factors for plants on acid soils, globally restricting agriculture. Despite its impact, little is known about Al action in planta. Earlier work has indicated that, among other effects, Al induces DNA damage. However, the loss of major DNA damage response regulators, such SOG1, partially suppressed the growth reduction in plants seen on Al‐containing media. This raised the question whether Al actually causes DNA damage and, if so, how. Here, we provide cytological and genetic data corroborating that exposure to Al leads to DNA double‐strand breaks. We find that the Al‐induced damage specifically involves homology‐dependent (HR) recombination repair. Using an Al toxicity assay that delivers higher Al concentrations than used in previous tests, we find that sog1 mutants become highly sensitive to Al. This indicates a multi‐level response to Al‐induced DNA damage in plants. |
format | Online Article Text |
id | pubmed-6850279 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-68502792019-11-18 A multi‐level response to DNA damage induced by aluminium Chen, Poyu Sjogren, Caroline A. Larsen, Paul B. Schnittger, Arp Plant J Original Articles Aluminium (Al) ions are one of the primary growth‐limiting factors for plants on acid soils, globally restricting agriculture. Despite its impact, little is known about Al action in planta. Earlier work has indicated that, among other effects, Al induces DNA damage. However, the loss of major DNA damage response regulators, such SOG1, partially suppressed the growth reduction in plants seen on Al‐containing media. This raised the question whether Al actually causes DNA damage and, if so, how. Here, we provide cytological and genetic data corroborating that exposure to Al leads to DNA double‐strand breaks. We find that the Al‐induced damage specifically involves homology‐dependent (HR) recombination repair. Using an Al toxicity assay that delivers higher Al concentrations than used in previous tests, we find that sog1 mutants become highly sensitive to Al. This indicates a multi‐level response to Al‐induced DNA damage in plants. John Wiley and Sons Inc. 2019-03-01 2019-05 /pmc/articles/PMC6850279/ /pubmed/30657222 http://dx.doi.org/10.1111/tpj.14231 Text en © 2019 The Authors. The Plant Journal published by John Wiley & Sons Ltd and Society for Experimental Biology. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Original Articles Chen, Poyu Sjogren, Caroline A. Larsen, Paul B. Schnittger, Arp A multi‐level response to DNA damage induced by aluminium |
title | A multi‐level response to DNA damage induced by aluminium |
title_full | A multi‐level response to DNA damage induced by aluminium |
title_fullStr | A multi‐level response to DNA damage induced by aluminium |
title_full_unstemmed | A multi‐level response to DNA damage induced by aluminium |
title_short | A multi‐level response to DNA damage induced by aluminium |
title_sort | multi‐level response to dna damage induced by aluminium |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6850279/ https://www.ncbi.nlm.nih.gov/pubmed/30657222 http://dx.doi.org/10.1111/tpj.14231 |
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