Cargando…

Pseudomonas putida KT2440 metabolism undergoes sequential modifications during exponential growth in a complete medium as compounds are gradually consumed

Pseudomonas putida is a soil bacterium with a versatile and robust metabolism. When confronted with mixtures of carbon sources, it prioritizes the utilization of the preferred compounds, optimizing metabolism and growth. This response is particularly strong when growing in a complex medium such as L...

Descripción completa

Detalles Bibliográficos
Autores principales: Molina, Lázaro, Rosa, Ruggero La, Nogales, Juan, Rojo, Fernando
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6850689/
https://www.ncbi.nlm.nih.gov/pubmed/30951237
http://dx.doi.org/10.1111/1462-2920.14622
_version_ 1783469482287562752
author Molina, Lázaro
Rosa, Ruggero La
Nogales, Juan
Rojo, Fernando
author_facet Molina, Lázaro
Rosa, Ruggero La
Nogales, Juan
Rojo, Fernando
author_sort Molina, Lázaro
collection PubMed
description Pseudomonas putida is a soil bacterium with a versatile and robust metabolism. When confronted with mixtures of carbon sources, it prioritizes the utilization of the preferred compounds, optimizing metabolism and growth. This response is particularly strong when growing in a complex medium such as LB. This work examines the changes occurring in P. putida KT2440 metabolic fluxes, while it grows exponentially in LB medium and sequentially consumes the compounds available. Integrating the uptake rates for each compound at three different moments during the exponential growth with the changes observed in the proteome, and with the metabolic fluxes predicted by the iJN1411 metabolic model for this strain, allowed the metabolic rearrangements that occurred to be determined. The results indicate that the bacterium changes significantly the configuration of its metabolism during the early, mid and late exponential phases of growth. Sugars served as an energy source during the early phase and later as energy and carbon source. The configuration of the tricarboxylic acids cycle varied during growth, providing no energy in the early phase, and turning to a reductive mode in the mid phase and to an oxidative mode later on. This work highlights the dynamism and flexibility of P. putida metabolism.
format Online
Article
Text
id pubmed-6850689
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher John Wiley & Sons, Inc.
record_format MEDLINE/PubMed
spelling pubmed-68506892019-11-18 Pseudomonas putida KT2440 metabolism undergoes sequential modifications during exponential growth in a complete medium as compounds are gradually consumed Molina, Lázaro Rosa, Ruggero La Nogales, Juan Rojo, Fernando Environ Microbiol Research Articles Pseudomonas putida is a soil bacterium with a versatile and robust metabolism. When confronted with mixtures of carbon sources, it prioritizes the utilization of the preferred compounds, optimizing metabolism and growth. This response is particularly strong when growing in a complex medium such as LB. This work examines the changes occurring in P. putida KT2440 metabolic fluxes, while it grows exponentially in LB medium and sequentially consumes the compounds available. Integrating the uptake rates for each compound at three different moments during the exponential growth with the changes observed in the proteome, and with the metabolic fluxes predicted by the iJN1411 metabolic model for this strain, allowed the metabolic rearrangements that occurred to be determined. The results indicate that the bacterium changes significantly the configuration of its metabolism during the early, mid and late exponential phases of growth. Sugars served as an energy source during the early phase and later as energy and carbon source. The configuration of the tricarboxylic acids cycle varied during growth, providing no energy in the early phase, and turning to a reductive mode in the mid phase and to an oxidative mode later on. This work highlights the dynamism and flexibility of P. putida metabolism. John Wiley & Sons, Inc. 2019-04-21 2019-07 /pmc/articles/PMC6850689/ /pubmed/30951237 http://dx.doi.org/10.1111/1462-2920.14622 Text en © 2019 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Research Articles
Molina, Lázaro
Rosa, Ruggero La
Nogales, Juan
Rojo, Fernando
Pseudomonas putida KT2440 metabolism undergoes sequential modifications during exponential growth in a complete medium as compounds are gradually consumed
title Pseudomonas putida KT2440 metabolism undergoes sequential modifications during exponential growth in a complete medium as compounds are gradually consumed
title_full Pseudomonas putida KT2440 metabolism undergoes sequential modifications during exponential growth in a complete medium as compounds are gradually consumed
title_fullStr Pseudomonas putida KT2440 metabolism undergoes sequential modifications during exponential growth in a complete medium as compounds are gradually consumed
title_full_unstemmed Pseudomonas putida KT2440 metabolism undergoes sequential modifications during exponential growth in a complete medium as compounds are gradually consumed
title_short Pseudomonas putida KT2440 metabolism undergoes sequential modifications during exponential growth in a complete medium as compounds are gradually consumed
title_sort pseudomonas putida kt2440 metabolism undergoes sequential modifications during exponential growth in a complete medium as compounds are gradually consumed
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6850689/
https://www.ncbi.nlm.nih.gov/pubmed/30951237
http://dx.doi.org/10.1111/1462-2920.14622
work_keys_str_mv AT molinalazaro pseudomonasputidakt2440metabolismundergoessequentialmodificationsduringexponentialgrowthinacompletemediumascompoundsaregraduallyconsumed
AT rosaruggerola pseudomonasputidakt2440metabolismundergoessequentialmodificationsduringexponentialgrowthinacompletemediumascompoundsaregraduallyconsumed
AT nogalesjuan pseudomonasputidakt2440metabolismundergoessequentialmodificationsduringexponentialgrowthinacompletemediumascompoundsaregraduallyconsumed
AT rojofernando pseudomonasputidakt2440metabolismundergoessequentialmodificationsduringexponentialgrowthinacompletemediumascompoundsaregraduallyconsumed