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Expression of VEGFA‐regulating miRNAs and mortality in wet AMD

MicroRNAs (miRNAs) regulate gene expression; many of them act in the retinal pigment epithelium (RPE), and RPE degeneration is known to be a critical factor in age‐related macular degeneration (AMD). Repeated injections with anti‐VEGFA (vascular endothelial growth factor A) are the only effective th...

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Autores principales: Blasiak, Janusz, Watala, Cezary, Tuuminen, Raimo, Kivinen, Niko, Koskela, Ali, Uusitalo‐Järvinen, Hannele, Tuulonen, Anja, Winiarczyk, Mateusz, Mackiewicz, Jerzy, Zmorzyński, Szymon, Filip, Agata, Kaarniranta, Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6850949/
https://www.ncbi.nlm.nih.gov/pubmed/31633290
http://dx.doi.org/10.1111/jcmm.14731
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author Blasiak, Janusz
Watala, Cezary
Tuuminen, Raimo
Kivinen, Niko
Koskela, Ali
Uusitalo‐Järvinen, Hannele
Tuulonen, Anja
Winiarczyk, Mateusz
Mackiewicz, Jerzy
Zmorzyński, Szymon
Filip, Agata
Kaarniranta, Kai
author_facet Blasiak, Janusz
Watala, Cezary
Tuuminen, Raimo
Kivinen, Niko
Koskela, Ali
Uusitalo‐Järvinen, Hannele
Tuulonen, Anja
Winiarczyk, Mateusz
Mackiewicz, Jerzy
Zmorzyński, Szymon
Filip, Agata
Kaarniranta, Kai
author_sort Blasiak, Janusz
collection PubMed
description MicroRNAs (miRNAs) regulate gene expression; many of them act in the retinal pigment epithelium (RPE), and RPE degeneration is known to be a critical factor in age‐related macular degeneration (AMD). Repeated injections with anti‐VEGFA (vascular endothelial growth factor A) are the only effective therapy in wet AMD. We investigated the correlation between the expression of 18 miRNAs involved in the regulation of the VEGFA gene in serum of 76 wet AMD patients and 70 controls. Efficacy of anti‐VEGFA treatment was evaluated by counting the number of injections delivered up to 12 years. In addition, we compared the relative numbers of deaths in patient with AMD and control groups. We observed a decreased expression of miR‐34‐5p, miR‐126‐3p, miR‐145‐5p and miR‐205‐5p in wet AMD patients as compared with controls. These miRNAs are involved in the regulation of angiogenesis, cytoprotection and protein clearance. No miRNA was significantly correlated with the treatment outcome. Wet AMD patients had greater mortality than controls, and their survival was inversely associated with the number of anti‐VEGFA injections per year. No association was observed between miRNA expression and mortality. Our study emphasizes the need to clarify the role of miRNA regulation in AMD pathogenesis.
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spelling pubmed-68509492019-12-01 Expression of VEGFA‐regulating miRNAs and mortality in wet AMD Blasiak, Janusz Watala, Cezary Tuuminen, Raimo Kivinen, Niko Koskela, Ali Uusitalo‐Järvinen, Hannele Tuulonen, Anja Winiarczyk, Mateusz Mackiewicz, Jerzy Zmorzyński, Szymon Filip, Agata Kaarniranta, Kai J Cell Mol Med Original Articles MicroRNAs (miRNAs) regulate gene expression; many of them act in the retinal pigment epithelium (RPE), and RPE degeneration is known to be a critical factor in age‐related macular degeneration (AMD). Repeated injections with anti‐VEGFA (vascular endothelial growth factor A) are the only effective therapy in wet AMD. We investigated the correlation between the expression of 18 miRNAs involved in the regulation of the VEGFA gene in serum of 76 wet AMD patients and 70 controls. Efficacy of anti‐VEGFA treatment was evaluated by counting the number of injections delivered up to 12 years. In addition, we compared the relative numbers of deaths in patient with AMD and control groups. We observed a decreased expression of miR‐34‐5p, miR‐126‐3p, miR‐145‐5p and miR‐205‐5p in wet AMD patients as compared with controls. These miRNAs are involved in the regulation of angiogenesis, cytoprotection and protein clearance. No miRNA was significantly correlated with the treatment outcome. Wet AMD patients had greater mortality than controls, and their survival was inversely associated with the number of anti‐VEGFA injections per year. No association was observed between miRNA expression and mortality. Our study emphasizes the need to clarify the role of miRNA regulation in AMD pathogenesis. John Wiley and Sons Inc. 2019-10-21 2019-12 /pmc/articles/PMC6850949/ /pubmed/31633290 http://dx.doi.org/10.1111/jcmm.14731 Text en © 2019 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Blasiak, Janusz
Watala, Cezary
Tuuminen, Raimo
Kivinen, Niko
Koskela, Ali
Uusitalo‐Järvinen, Hannele
Tuulonen, Anja
Winiarczyk, Mateusz
Mackiewicz, Jerzy
Zmorzyński, Szymon
Filip, Agata
Kaarniranta, Kai
Expression of VEGFA‐regulating miRNAs and mortality in wet AMD
title Expression of VEGFA‐regulating miRNAs and mortality in wet AMD
title_full Expression of VEGFA‐regulating miRNAs and mortality in wet AMD
title_fullStr Expression of VEGFA‐regulating miRNAs and mortality in wet AMD
title_full_unstemmed Expression of VEGFA‐regulating miRNAs and mortality in wet AMD
title_short Expression of VEGFA‐regulating miRNAs and mortality in wet AMD
title_sort expression of vegfa‐regulating mirnas and mortality in wet amd
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6850949/
https://www.ncbi.nlm.nih.gov/pubmed/31633290
http://dx.doi.org/10.1111/jcmm.14731
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