Transcription and translation contribute to gene locus relocation to the nucleoid periphery in E. coli

Transcription by RNA polymerase (RNAP) is coupled with translation in bacteria. Here, we observe the dynamics of transcription and subcellular localization of a specific gene locus (encoding a non-membrane protein) in living E. coli cells at subdiffraction-limit resolution. The movement of the gene...

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Autores principales: Yang, Sora, Kim, Seunghyeon, Kim, Dong-Kyun, Jeon An, Hyeong, Bae Son, Jung, Hedén Gynnå, Arvid, Ki Lee, Nam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6851099/
https://www.ncbi.nlm.nih.gov/pubmed/31719538
http://dx.doi.org/10.1038/s41467-019-13152-y
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author Yang, Sora
Kim, Seunghyeon
Kim, Dong-Kyun
Jeon An, Hyeong
Bae Son, Jung
Hedén Gynnå, Arvid
Ki Lee, Nam
author_facet Yang, Sora
Kim, Seunghyeon
Kim, Dong-Kyun
Jeon An, Hyeong
Bae Son, Jung
Hedén Gynnå, Arvid
Ki Lee, Nam
author_sort Yang, Sora
collection PubMed
description Transcription by RNA polymerase (RNAP) is coupled with translation in bacteria. Here, we observe the dynamics of transcription and subcellular localization of a specific gene locus (encoding a non-membrane protein) in living E. coli cells at subdiffraction-limit resolution. The movement of the gene locus to the nucleoid periphery correlates with transcription, driven by either E. coli RNAP or T7 RNAP, and the effect is potentiated by translation.
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spelling pubmed-68510992019-11-14 Transcription and translation contribute to gene locus relocation to the nucleoid periphery in E. coli Yang, Sora Kim, Seunghyeon Kim, Dong-Kyun Jeon An, Hyeong Bae Son, Jung Hedén Gynnå, Arvid Ki Lee, Nam Nat Commun Article Transcription by RNA polymerase (RNAP) is coupled with translation in bacteria. Here, we observe the dynamics of transcription and subcellular localization of a specific gene locus (encoding a non-membrane protein) in living E. coli cells at subdiffraction-limit resolution. The movement of the gene locus to the nucleoid periphery correlates with transcription, driven by either E. coli RNAP or T7 RNAP, and the effect is potentiated by translation. Nature Publishing Group UK 2019-11-12 /pmc/articles/PMC6851099/ /pubmed/31719538 http://dx.doi.org/10.1038/s41467-019-13152-y Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yang, Sora
Kim, Seunghyeon
Kim, Dong-Kyun
Jeon An, Hyeong
Bae Son, Jung
Hedén Gynnå, Arvid
Ki Lee, Nam
Transcription and translation contribute to gene locus relocation to the nucleoid periphery in E. coli
title Transcription and translation contribute to gene locus relocation to the nucleoid periphery in E. coli
title_full Transcription and translation contribute to gene locus relocation to the nucleoid periphery in E. coli
title_fullStr Transcription and translation contribute to gene locus relocation to the nucleoid periphery in E. coli
title_full_unstemmed Transcription and translation contribute to gene locus relocation to the nucleoid periphery in E. coli
title_short Transcription and translation contribute to gene locus relocation to the nucleoid periphery in E. coli
title_sort transcription and translation contribute to gene locus relocation to the nucleoid periphery in e. coli
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6851099/
https://www.ncbi.nlm.nih.gov/pubmed/31719538
http://dx.doi.org/10.1038/s41467-019-13152-y
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