Cargando…

Detection of Campylobacter spp. in water by dead‐end ultrafiltration and application at farm level

AIMS: The purposes were to evaluate the detection of low levels of Campylobacter in water by dead‐end ultrafiltration (DEUF) to determine the sensitivity and suitability for use under field condition. METHODS AND RESULTS: The DEUF technique followed by detection according to ISO 10272 was tested on...

Descripción completa

Detalles Bibliográficos
Autores principales: Ferrari, S., Frosth, S., Svensson, L., Fernström, L.‐L., Skarin, H., Hansson, I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6851547/
https://www.ncbi.nlm.nih.gov/pubmed/31291690
http://dx.doi.org/10.1111/jam.14379
_version_ 1783469640996880384
author Ferrari, S.
Frosth, S.
Svensson, L.
Fernström, L.‐L.
Skarin, H.
Hansson, I.
author_facet Ferrari, S.
Frosth, S.
Svensson, L.
Fernström, L.‐L.
Skarin, H.
Hansson, I.
author_sort Ferrari, S.
collection PubMed
description AIMS: The purposes were to evaluate the detection of low levels of Campylobacter in water by dead‐end ultrafiltration (DEUF) to determine the sensitivity and suitability for use under field condition. METHODS AND RESULTS: The DEUF technique followed by detection according to ISO 10272 was tested on artificially and naturally contaminated water. Campylobacter were detected in all samples spiked with more than 10 CFU 60 l(−1) and in four of nine samples with a concentration below 10 CFU 60 l(−1) water. Naturally contaminated water from five different broiler producers was analysed. Campylobacter were detected in four of 12 samples from ponds near the houses and in three of 24 samples from water pipes inside the broiler houses, but not in tap water sampled at the entrance of the broiler houses. CONCLUSIONS: The results indicate that DEUF is useful for detection of low numbers of Campylobacter in large volumes of water. SIGNIFICANCE AND IMPACT OF THE STUDY: Contaminated water is an important source for transmission of Campylobacter to broilers and humans. The concentration of Campylobacter is usually low with a high level of background microbiota. This study shows the advantages of DEUF both in the laboratory and under field conditions.
format Online
Article
Text
id pubmed-6851547
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-68515472019-11-18 Detection of Campylobacter spp. in water by dead‐end ultrafiltration and application at farm level Ferrari, S. Frosth, S. Svensson, L. Fernström, L.‐L. Skarin, H. Hansson, I. J Appl Microbiol Original Articles AIMS: The purposes were to evaluate the detection of low levels of Campylobacter in water by dead‐end ultrafiltration (DEUF) to determine the sensitivity and suitability for use under field condition. METHODS AND RESULTS: The DEUF technique followed by detection according to ISO 10272 was tested on artificially and naturally contaminated water. Campylobacter were detected in all samples spiked with more than 10 CFU 60 l(−1) and in four of nine samples with a concentration below 10 CFU 60 l(−1) water. Naturally contaminated water from five different broiler producers was analysed. Campylobacter were detected in four of 12 samples from ponds near the houses and in three of 24 samples from water pipes inside the broiler houses, but not in tap water sampled at the entrance of the broiler houses. CONCLUSIONS: The results indicate that DEUF is useful for detection of low numbers of Campylobacter in large volumes of water. SIGNIFICANCE AND IMPACT OF THE STUDY: Contaminated water is an important source for transmission of Campylobacter to broilers and humans. The concentration of Campylobacter is usually low with a high level of background microbiota. This study shows the advantages of DEUF both in the laboratory and under field conditions. John Wiley and Sons Inc. 2019-07-22 2019-10 /pmc/articles/PMC6851547/ /pubmed/31291690 http://dx.doi.org/10.1111/jam.14379 Text en © 2019 The Authors. Journal of Applied Microbiology published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Ferrari, S.
Frosth, S.
Svensson, L.
Fernström, L.‐L.
Skarin, H.
Hansson, I.
Detection of Campylobacter spp. in water by dead‐end ultrafiltration and application at farm level
title Detection of Campylobacter spp. in water by dead‐end ultrafiltration and application at farm level
title_full Detection of Campylobacter spp. in water by dead‐end ultrafiltration and application at farm level
title_fullStr Detection of Campylobacter spp. in water by dead‐end ultrafiltration and application at farm level
title_full_unstemmed Detection of Campylobacter spp. in water by dead‐end ultrafiltration and application at farm level
title_short Detection of Campylobacter spp. in water by dead‐end ultrafiltration and application at farm level
title_sort detection of campylobacter spp. in water by dead‐end ultrafiltration and application at farm level
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6851547/
https://www.ncbi.nlm.nih.gov/pubmed/31291690
http://dx.doi.org/10.1111/jam.14379
work_keys_str_mv AT ferraris detectionofcampylobactersppinwaterbydeadendultrafiltrationandapplicationatfarmlevel
AT frosths detectionofcampylobactersppinwaterbydeadendultrafiltrationandapplicationatfarmlevel
AT svenssonl detectionofcampylobactersppinwaterbydeadendultrafiltrationandapplicationatfarmlevel
AT fernstromll detectionofcampylobactersppinwaterbydeadendultrafiltrationandapplicationatfarmlevel
AT skarinh detectionofcampylobactersppinwaterbydeadendultrafiltrationandapplicationatfarmlevel
AT hanssoni detectionofcampylobactersppinwaterbydeadendultrafiltrationandapplicationatfarmlevel