Impact of cold storage on platelets treated with Intercept pathogen inactivation

BACKGROUND: Pathogen inactivation and cold or cryopreservation of platelets (PLTs) both significantly affect PLT function. It is not known how PLTs function when both are combined. STUDY DESIGN AND METHODS: Standard PLT concentrates (PCs) were compared to pathogen‐inactivated PCs treated with amotosalen photochemical treatment (AS‐PCT) when stored at room (RT, 22°C), cold (4°C, n = 6), or cryopreservation (−80°C, n = 8) temperatures. The impact of alternative storage methods on both arms was studied in flow cytometry, light transmittance aggregometry, and hemostasis in collagen‐coated microfluidic flow chambers. RESULTS: Platelet aggregation of cold‐stored AS‐PCT PLTs was 44% ± 11% compared to 57% ± 14% for cold‐stored standard PLTs and 58% ± 21% for RT‐stored AS‐PCT PLTs. Integrin activation of cold‐stored AS‐PCT PLTs was 53% ± 9% compared to 77% ± 6% for cold‐stored standard PLTs and 69% ± 13% for RT‐stored AS‐PCT PLTs. Coagulation of cold‐stored AS‐PCT PLTs started faster under flow (836 ± 140 sec) compared to cold‐stored standard PLTs (960 ± 192 sec) and RT‐stored AS‐PCT PLTs (1134 ± 220 sec). Fibrin formation rate under flow was also highest for cold‐stored AS‐PCT PLTs. This was in line with thrombin generation in static conditions because cold‐stored AS‐PCT PLTs generated 297 ± 47 nmol/L thrombin compared to 159 ± 33 nmol/L for cold‐stored standard PLTs and 83 ± 25 nmol/L for RT‐stored AS‐PCT PLTs. So despite decreased PLT activation and aggregation, cold storage of AS‐PCT PLTs promoted coagulation. PLT aggregation of cryopreserved AS‐PCT PLTs (23% ± 10%) was not significantly different from cryopreserved standard PLTs (25% ± 8%). CONCLUSION: This study shows that cold storage of AS‐PCT PLTs further affects PLT activation and aggregation but promotes (pro)coagulation. Increased procoagulation was not observed after cryopreservation.