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Minireview: demystifying microbial reaction energetics
The biology literature is rife with misleading information on how to quantify catabolic reaction energetics. The principal misconception is that the sign and value of the standard Gibbs energy ([Formula: see text]) define the direction and energy yield of a reaction; they do not. [Formula: see text]...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6852080/ https://www.ncbi.nlm.nih.gov/pubmed/31403238 http://dx.doi.org/10.1111/1462-2920.14778 |
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author | Amend, Jan P. LaRowe, Douglas E. |
author_facet | Amend, Jan P. LaRowe, Douglas E. |
author_sort | Amend, Jan P. |
collection | PubMed |
description | The biology literature is rife with misleading information on how to quantify catabolic reaction energetics. The principal misconception is that the sign and value of the standard Gibbs energy ([Formula: see text]) define the direction and energy yield of a reaction; they do not. [Formula: see text] is one part of the actual Gibbs energy of a reaction (ΔG(r)), with a second part accounting for deviations from the standard composition. It is also frequently assumed that [Formula: see text] applies only to 25 °C and 1 bar; it does not. [Formula: see text] is a function of temperature and pressure. Here, we review how to determine ΔG(r) as a function of temperature, pressure and chemical composition for microbial catabolic reactions, including a discussion of the effects of ionic strength on ΔG(r) and highlighting the large effects when multi‐valent ions are part of the reaction. We also calculate ΔG(r) for five example catabolisms at specific environmental conditions: aerobic respiration of glucose in freshwater, anaerobic respiration of acetate in marine sediment, hydrogenotrophic methanogenesis in a laboratory batch reactor, anaerobic ammonia oxidation in a wastewater reactor and aerobic pyrite oxidation in acid mine drainage. These examples serve as templates to determine the energy yields of other catabolic reactions at environmentally relevant conditions. |
format | Online Article Text |
id | pubmed-6852080 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley & Sons, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-68520802019-11-18 Minireview: demystifying microbial reaction energetics Amend, Jan P. LaRowe, Douglas E. Environ Microbiol Minireview The biology literature is rife with misleading information on how to quantify catabolic reaction energetics. The principal misconception is that the sign and value of the standard Gibbs energy ([Formula: see text]) define the direction and energy yield of a reaction; they do not. [Formula: see text] is one part of the actual Gibbs energy of a reaction (ΔG(r)), with a second part accounting for deviations from the standard composition. It is also frequently assumed that [Formula: see text] applies only to 25 °C and 1 bar; it does not. [Formula: see text] is a function of temperature and pressure. Here, we review how to determine ΔG(r) as a function of temperature, pressure and chemical composition for microbial catabolic reactions, including a discussion of the effects of ionic strength on ΔG(r) and highlighting the large effects when multi‐valent ions are part of the reaction. We also calculate ΔG(r) for five example catabolisms at specific environmental conditions: aerobic respiration of glucose in freshwater, anaerobic respiration of acetate in marine sediment, hydrogenotrophic methanogenesis in a laboratory batch reactor, anaerobic ammonia oxidation in a wastewater reactor and aerobic pyrite oxidation in acid mine drainage. These examples serve as templates to determine the energy yields of other catabolic reactions at environmentally relevant conditions. John Wiley & Sons, Inc. 2019-08-27 2019-10 /pmc/articles/PMC6852080/ /pubmed/31403238 http://dx.doi.org/10.1111/1462-2920.14778 Text en © 2019 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Minireview Amend, Jan P. LaRowe, Douglas E. Minireview: demystifying microbial reaction energetics |
title | Minireview: demystifying microbial reaction energetics |
title_full | Minireview: demystifying microbial reaction energetics |
title_fullStr | Minireview: demystifying microbial reaction energetics |
title_full_unstemmed | Minireview: demystifying microbial reaction energetics |
title_short | Minireview: demystifying microbial reaction energetics |
title_sort | minireview: demystifying microbial reaction energetics |
topic | Minireview |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6852080/ https://www.ncbi.nlm.nih.gov/pubmed/31403238 http://dx.doi.org/10.1111/1462-2920.14778 |
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